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Kidney organ chip and kidney organ medicine detection model

An organ chip and chip technology, applied in the field of microfluidics, can solve the problems of large consumption of reagents, inaccurate flow rate control, and influence on experimental results, etc., and achieve the effect of rapid sample consumption, simple operation, and high cell survival rate

Inactive Publication Date: 2019-06-11
百澳瑞派(天津)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

All of the above methods have problems such as large reagent consumption and inaccurate flow rate control, and are only suitable for studying the biological behavior of cells on a two-dimensional plane. It is difficult to imitate the complex microenvironment in vivo, which seriously affects the experimental results.

Method used

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  • Kidney organ chip and kidney organ medicine detection model
  • Kidney organ chip and kidney organ medicine detection model
  • Kidney organ chip and kidney organ medicine detection model

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0137] Experimental example 1. Performance detection of the microfluidic chip of the present invention

[0138] The present invention has carried out a research experiment on the performance of the microfluidic chip of the present invention and the existing common chip (for example: the microfluidic chip recorded in the invention patent application 201410191283, 201510860959), except that the chip is different, other conditions are the same, including Using the same cells (specifically, human proximal tubular epithelial cells RPTECs), under the same culture conditions (the medium, methods and culture conditions used in the specific cell culture process are carried out with reference to the product instructions of commercially available RPTECs) Cell culture was carried out to obtain the following comparative data:

[0139] chip type

Initial amount of sample

Liquid change frequency

cell viability

Chip of the present invention

30ml

more than 7 ...

experiment example 2

[0142] Experimental example 2, kidney organ chip and drug detection model of the present invention

[0143] (1) Kidney organ chip

[0144] Such as Figure 1 to Figure 5 Shown: a microfluidic chip, which includes a porous polycarbonate membrane layer 1, the two sides of the porous polycarbonate membrane layer 1 are respectively provided with a chip upper layer 2 and a chip lower layer 3, that is, the porous polycarbonate membrane Layer 1 is sandwiched between the upper chip layer 2 and the lower chip layer 3, and the upper chip layer 2 and the lower chip layer 3 are both made of polydimethylsiloxane (or acrylic plate);

[0145] On the upper layer of the chip (3), there are an upper inlet pool (6), a diamond-shaped upper cell culture chamber (7), an upper outlet pool (8), and a middle inlet pool (11), a middle The outlet pond (13), the lower inlet pond (16) and the lower outlet pond (18), are communicated with the first passage (9) between the upper inlet pond (6) and the uppe...

experiment example 3

[0156] Experimental example 3. Detection of albumin metabolites in renal tubular RPTEC cells

[0157] The increasing prevalence of diabetes mellitus (DM) and secondary kidney injury leads to diabetic nephropathy (DN). Early renal disease is defined as microalbuminuria (30-300mg / day), so the detection of albumin in renal organ metabolites is particularly important.

[0158] The detection of renal cell metabolites is an effective indicator to prove that the cells are growing well. Albumin content in cell metabolites was determined using a microplate reader using an albumin detection kit (bromocresol green colorimetric method). Such as Figure 10 As shown, the original albumin content in the detection medium was 2.22mg / ml, after 3 days the albumin content was 2.353mg / ml, after 6 days the albumin content was 2.704mg / ml, after 9 days the albumin content was 2.745mg / ml . On the 3rd and 6th days, the albumin metabolism was the most, the cell growth was better, and the metabolism ...

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Abstract

The invention relates to a kidney organ chip and a kidney organ medicine detection model, and belongs to the technical field of microflow control. The kidney organ chip comprises at least 2 chip layers and porous layers for separating the chip layers, wherein each porous layer is located between every two chip layers; a cell culture room, an entrance pond, an entrance passage, an exit pond and anexit passage are arranged on the upper surface of each chip layer; and each cell culture room is a large depressed area arranged on the upper surface of the corresponding chip layer and is used for providing three-dimensional culture space for cells. The chip has the advantages of being low in sample dosage, high in culture efficiency, simple to operate, high in cell viability and the like when being used for cell culture.

Description

technical field [0001] The invention belongs to the technical field of microfluidics, and in particular relates to a kidney organ chip and a kidney organ drug detection model. Background technique [0002] In order to better imitate the microenvironment in which cells live in the human body, only by creating a co-culture system of multiple cells can the partial functions of human organs be reconstructed in vitro. Therefore, there is an urgent need for a new technique to co-culture several types of cells. Cell co-culture is an important means to study the interaction between cells. At present, there are mainly the following methods for cell co-culture research: 1) Mixed culture; 2) Conditioned medium culture; 3) Microcarrier culture method; 4) Microwell Bottom membrane dish culture method. All of the above methods have problems such as large reagent consumption and inaccurate flow rate control, and are only suitable for studying the biological behavior of cells on a two-dim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12N5/00
Inventor 楊士模尹棣杜冠儒殷磊
Owner 百澳瑞派(天津)生物科技有限公司
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