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HPLC fingerprint for ethyl acetate position of longan leaf and establishing method of HPLC fingerprint

A fingerprint, ethyl acetate technology, applied in measuring devices, instruments, scientific instruments, etc., to achieve good separation effect, rich chromatographic peak information, and improve the standard of medicinal materials.

Active Publication Date: 2019-06-07
GUANGXI UNIV OF CHINESE MEDICINE
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Problems solved by technology

In order to further improve the standards of longan leaf medicinal materials, promote the standardization of traditional Chinese medicine, ensure and improve the quality of longan leaf medicinal materials, and provide accurate and reliable evidence for the safety and effectiveness of clinical medication, the inventors have studied longan leaf anti-type Ⅱ on the basis of previous research on longan leaf. The effective part of diabetes - the fingerprint of the ethyl acetate part has been studied, but there is no relevant report so far

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  • HPLC fingerprint for ethyl acetate position of longan leaf and establishing method of HPLC fingerprint
  • HPLC fingerprint for ethyl acetate position of longan leaf and establishing method of HPLC fingerprint
  • HPLC fingerprint for ethyl acetate position of longan leaf and establishing method of HPLC fingerprint

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Embodiment Construction

[0029] The present invention will be further described below in conjunction with specific embodiment, but do not limit protection scope and application scope of the present invention:

[0030] The invention provides a method for establishing the HPLC fingerprint of the ethyl acetate part of longan leaves, comprising the following steps:

[0031] S1. Preparation of the test solution: take 2 g of longan leaf coarse powder, accurately weigh it, put it in a stoppered conical flask, first add 20ml of 95% ethanol for ultrasonication for 1h, then add 20ml of 50% ethanol for ultrasonication for 30min, filter, and evaporate the filtrate to dryness , add water to suspend in a separatory funnel, add an equal amount of petroleum ether and repeatedly extract until the extracted petroleum ether layer is colorless, take the lower layer solution, add an equal amount of ethyl acetate and repeatedly extract until the extracted ethyl acetate layer is colorless color, combined the ethyl acetate l...

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Abstract

The invention discloses an HPLC fingerprint for the ethyl acetate position of a longan leaf and an establishing method of the HPLC fingerprint. The method comprises the steps that a solution of the tested object is prepared; a contrast solution including ethyl gallate, astragalin and quercetin is prepared; and HPLC is used for determination to obtain the fingerprint. The method has the advantagesof being rapid, accurate, high in precision, repeatability and stability, abundant in chromatographic peak information, and good in separating effect. The established HPLC fingerprint for the ethyl acetate position of the longan leaf can effectively represent the quality of the longan leaf, especially the quality of the ethyl acetate position of the longan leaf, and furhter the medical standard ofthe longan leaf is improved, the longan leaf quality evaluation system is improved, standardization of traditional Chinese medicines is facilitated, theoretical and practical basis is provided for comprehensive and effective control for the longan leaf quality, and accurate reliable basis is provided for the safety and effectiveness of clinical medicine.

Description

technical field [0001] The quality control method of the traditional Chinese medicinal material of the invention specifically relates to a method for constructing a high-performance liquid chromatography fingerprint of the ethyl acetate part of longan leaves and a standard fingerprint. Background technique [0002] Longan leaf is a traditional Chinese medicinal material with Guangxi national characteristics, which was first recorded in "Southern Yunnan Bencao Tushuo". It is the leaves or shoots of longan belonging to Sapindaceae longan. Guangxi, Guangdong, Fujian and Taiwan are the main producing areas, and Hainan, Yunnan, Sichuan and Guizhou are also cultivated. Its nature and flavor are sweet, light, and flat, and it has the functions of clearing heat, dampness and detoxification. It is mainly used to treat cold, fever, malaria, boils, eczema, etc. Longan leaves mainly contain tannins, phenolic acids, flavonoids and volatile oils. At present, there are not many domestic...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCY02A50/30
Inventor 梁洁黄春燕周昱杉孙正伊徐晖麦嘉妮
Owner GUANGXI UNIV OF CHINESE MEDICINE
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