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Ferrum-copper composite nanoflowers containing papain and preparation method and application of ferrum-copper composite nanoflowers

A papain and composite magnetic technology, applied in applications, magnetic materials, magnetic objects, etc., can solve the problems of not being too severe, the structure of nano-flowers is fragile, etc., to reduce sensitization, improve reuse performance, and quickly separate the reaction solution. Effect

Active Publication Date: 2019-06-07
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the structure of nanoflowers is relatively fragile, and the separation process should not be too violent

Method used

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  • Ferrum-copper composite nanoflowers containing papain and preparation method and application of ferrum-copper composite nanoflowers
  • Ferrum-copper composite nanoflowers containing papain and preparation method and application of ferrum-copper composite nanoflowers
  • Ferrum-copper composite nanoflowers containing papain and preparation method and application of ferrum-copper composite nanoflowers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] (1) Dissolve 1.2g of sodium citrate in 80mL of ethylene glycol, add 2.6g of ferric chloride and stir until dissolved, then add 3.2g of sodium acetate and stir for 30 minutes, and keep the above solution at 200°C for 20 hours. After the reaction, wash with water for 3 times and ethanol for 5 times, then dry naturally, and the obtained black powder is ferroferric oxide magnetic particles.

[0056] (2) Take 300 mg of the ferroferric oxide magnetic particle obtained in step (1) and add it to 100 g of 400 mmol / L urea aqueous solution for ultrasonication for 1 hour, then add 1.25 g of cetyltrimethylammonium bromide, 1.25 g of n-butanol and 5 g of Cyclohexane, after stirring evenly, add 0.875g tetraethyl orthosilicate and 0.375g 3-aminopropyltriethoxysilane. The mixture was reacted at 70° C. for 24 hours, and after the reaction was completed, the precipitate was refluxed with acetone at 80° C. for 24 hours. Finally, the precipitate is collected, washed, magnetically separated...

Embodiment 2

[0065] (1) Dissolve 1.2g of sodium citrate in 80mL of ethylene glycol, add 2.6g of ferric chloride and stir until dissolved, then add 3.2g of sodium acetate and stir for 30 minutes, and keep the above solution at 200°C for 20 hours. After the reaction, wash with water for 3 times and ethanol for 5 times, then dry naturally, and the obtained black powder is ferroferric oxide magnetic particles.

[0066] (2) Take 300 mg of the ferroferric oxide magnetic particle obtained in step (1) and add it to 100 g of 400 mmol / L urea aqueous solution for ultrasonication for 1 hour, then add 1.25 g of cetyltrimethylammonium bromide, 1.25 g of n-butanol and 5 g of Cyclohexane, after stirring evenly, add 0.875g tetraethyl orthosilicate and 0.375g 3-aminopropyltriethoxysilane. The mixture was reacted at 70° C. for 24 hours, and after the reaction was completed, the precipitate was refluxed with acetone at 80° C. for 24 hours. Finally, the precipitate is collected, washed, magnetically separated...

Embodiment 3

[0070] (1) Dissolve 1.2g of sodium citrate in 80mL of ethylene glycol, add 2.6g of ferric chloride and stir until dissolved, then add 3.2g of sodium acetate and stir for 30 minutes, and keep the above solution at 200°C for 20 hours. After the reaction, wash with water for 3 times and ethanol for 5 times, then dry naturally, and the obtained black powder is ferroferric oxide magnetic particles.

[0071] (2) Take 300 mg of the ferroferric oxide magnetic particle obtained in step (1) and add it to 100 g of 400 mmol / L urea aqueous solution for ultrasonication for 1 hour, then add 1.25 g of cetyltrimethylammonium bromide, 1.25 g of n-butanol and 5 g of Cyclohexane, after stirring evenly, add 0.875g tetraethyl orthosilicate and 0.375g 3-aminopropyltriethoxysilane. The mixture was reacted at 70° C. for 24 hours, and after the reaction was completed, the precipitate was refluxed with acetone at 80° C. for 24 hours. Finally, the precipitate is collected, washed, magnetically separated...

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Abstract

The invention relates to ferrum-copper composite nanoflowers containing papain and a preparation method and application of the ferrum-copper composite nanoflowers, and belongs to the field of enzyme fossilization. The preparation method of the ferrum-copper composite nanoflowers containing papain comprises the following steps of mixing a magnetic Fe3O4 nanoparticle solution modified with amino with a papain solution, and then adding a copper ion inorganic salt solution to obtain the ferrum-copper composite nanoflowers containing papain. The synthesized papain ferrum-copper composite magnetic nanoflowers have higher specific surface area than free enzymes, so that the mass transfer obstruction between the enzymes and a substrate is further reduced, and the enzymatic activity of fossilized papain is about 25 times of that of free enzymes.

Description

technical field [0001] The invention relates to an iron-copper composite magnetic nano flower containing papain and a preparation method and application thereof, belonging to the field of enzyme immobilization. Background technique [0002] Papain, also known as papain, is widely found in the roots, stems, leaves and fruits of papaya, among which the immature milk is the most abundant. The active center of papain contains cysteine, which belongs to sulfhydryl protease. At the same time, papain has a wide range of specificity, and has strong hydrolysis ability to animal and plant proteins, polypeptides, lipids, amides, etc. In addition, papain can decompose protein in acidic, neutral and alkaline environments. Not only that, but its heat resistance is also very good. Although its optimum temperature is 55-65°C, it has catalytic activity in the temperature range of 10-85°C. In the food industry, papain is mainly used in meat tenderization, beer clarification and other field...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14H01F1/00H01F41/00A23C9/12
Inventor 田晶张海洋费旭李尧徐龙权王一
Owner DALIAN POLYTECHNIC UNIVERSITY
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