A kind of preparation method of concrete repair mortar
A technology for repairing mortar and concrete, applied to biochemical equipment and methods, methods based on microorganisms, fixed on/in organic carriers, etc., can solve the problem of difficulty in maintaining the repair effect, uneven calcium carbonate, and concrete bearing capacity and durability reduction, etc., to achieve good repair effect, protect metabolic process, and improve compressive strength
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Embodiment 1
[0045] Prepare concrete repair mortar as follows.
[0046](1) Add 200 ml of 5 mol / L citric acid to 100 g of corn stalk powder (particle size 60 μm), acidify for 2 hours, wash with distilled water until neutral, and dry to obtain acidified straw powder;
[0047] (2) Mix the strain cells, 15% urea solution, 50% calcium acetate solution and 2% sodium alginate solution in a weight ratio of 1:5:6:5, stir at 30°C for 20 minutes, let stand for 32 hours, and mix according to the weight ratio Solution: acidified straw=10:2, add the acidified straw powder obtained in step (1), mix evenly, let it stand for 2 hours, and obtain the repairing liquid, wherein, the strain cells are purified carbonic anhydrase strains in a sterile medium After culturing at 30°C for 48 hours, it was obtained by repeated centrifugation and washing at 2°C. The sterile medium was composed of: peptone 15g / L, yeast powder 20g / L, ammonium sulfate 10g / L, pH 7;
[0048] (3) Mix the repair liquid obtained in step (2) w...
Embodiment 2
[0050] Prepare concrete repair mortar as follows.
[0051] (1) Add 300 ml of 5 mol / L citric acid to 100 g of corn stalk powder (particle size 100 μm), acidify for 1.5 h, wash with distilled water until neutral, and dry to obtain acidified straw powder;
[0052] (2) Mix the strain cells, 15% urea solution, 50% calcium acetate solution and 2% sodium alginate solution in a weight ratio of 1:3:8:6, stir at 30°C for 20 minutes, let stand for 48 hours, and mix according to the weight ratio Solution: acidified straw=10:4, add the acidified straw powder obtained in step (1), mix evenly, and let it stand for 3 hours to obtain a repairing solution, wherein the strain cells are purified carbonic anhydrase strains in a sterile medium After culturing at 30°C for 24 hours, it was obtained by repeated centrifugation and washing at 2°C. The sterile medium consists of: meat extract broth 30g / L, peptone 12g / L, polysaccharide 8g / L, agar 10g / L, pH is 6.5;
[0053] (3) Mix the repair liquid obta...
Embodiment 3
[0055] Prepare concrete repair mortar as follows.
[0056] (1) Add 200 ml of 5 mol / L citric acid to 100 g of corn stalk powder (particle size 120 μm), acidify for 1.5 h, wash with distilled water until neutral, and dry to obtain acidified straw powder;
[0057] (2) Mix the strain cells, 15% urea solution, 50% calcium acetate solution and 2% sodium alginate solution in a weight ratio of 1:4:8:5, stir at 30°C for 20 minutes, let stand for 48 hours, and mix according to the weight ratio Solution: acidified straw=10:3.5 add the acidified straw powder obtained in step (1), after mixing evenly, let it stand for 3 hours to obtain repairing solution, wherein, the strain cells are purified carbonic anhydrase strains in a sterile medium After culturing at 30°C for 48 hours, it was obtained by repeated centrifugation and water washing at 2°C. The sterile medium consists of: meat extract broth 30g / L, peptone 12g / L, polysaccharide 8g / L, agar 10g / L, pH is 6.5;
[0058] (3) Mix the repair ...
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