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Urine protein marker for malignant hypertensive renal injury and diagnostic use thereof

A technology for kidney injury and high blood pressure, applied in the field of clinical medicine, can solve the problems of easy bleeding in renal puncture, avoid the risk of kidney biopsy and make a clear diagnosis

Active Publication Date: 2019-06-04
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, patients with malignant hypertension are prone to bleeding during renal puncture due to high blood pressure and arteriosclerosis, so renal biopsy needs to be very cautious

Method used

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  • Urine protein marker for malignant hypertensive renal injury and diagnostic use thereof
  • Urine protein marker for malignant hypertensive renal injury and diagnostic use thereof
  • Urine protein marker for malignant hypertensive renal injury and diagnostic use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0039] 1. Materials and Reagents

[0040] 1) Instrument

[0041] Triple TOF TM 5600 mass spectrometer was purchased from AB SCIEX company.

[0042] 2) Main reagents

[0043] Deionized water was obtained from MillliQ RG ultrapure water system; mass spectrometry grade water, acetonitrile, formic acid and methanol were purchased from Fisher; iodoacetamide (IAA), ammonium bicarbonate, urea, dithiothreitol (DTT) were purchased from Sigma Purchase; gold trypsin was purchased from Promega.

[0044] 2. Experimental method

[0045] 1) Collect a urine sample

[0046] Inpatients with malignant hypertensive renal injury (9 cases), membranous nephropathy (3 cases), systemic lupus erythematosus nephritis (3 cases), IgA nephritis (3 cases), Henoch-Schonlein purpura nephritis ( 3 cases), and healthy controls (3 cases) in the physical examination clinic. After obtaining informed consent, each subject collected about 50ml of mid-morning urine.

[0047] 2) Acetone precipitation method t...

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PUM

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Abstract

The invention discloses a urine protein marker for malignant hypertensive renal injury and a diagnostic application thereof. Specifically, the invention discloses the application of an identificationreagent for proteins selected from the following: a complement factor D, pancreatic ribonuclease, pancreatic stone protein-1-alpha, retinol conjugated protein 4, aggregin and a combination thereof, inpreparation of reagents for diagnosis of the malignant hypertensive renal injury of subjects. Herein, the protein marker has good clinical application prospects in the diagnosis of the malignant hypertensive renal injury.

Description

technical field [0001] The invention relates to clinical medicine; in particular, it relates to a urine protein marker related to human malignant hypertensive kidney injury. More specifically, the present invention relates to urine protein markers related to the diagnosis of human malignant hypertensive renal injury obtained by using the urine of patients with malignant hypertensive renal injury and mass spectrometry proteomics technology, and its use. Background technique [0002] Disease biomarkers are measurable changes associated with pathophysiological processes that can be used for disease diagnosis, monitoring, prognosis assessment, etc. [1] . [0003] Urine is formed by the filtration of blood by the glomerulus and the secretion of the renal tubules, collecting ducts, and urinary tract. As a waste product produced by the body's metabolism, urine is not regulated by the homeostasis mechanism, so urine can enrich various changes excreted by the body, and these change...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N27/62G01N30/02G01N27/447
Inventor 高友鹤秦伟伟李明喜林丽玲
Owner BEIJING NORMAL UNIVERSITY
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