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Collagen film with 3D micro-patterns, and preparation method and application thereof

A micro-pattern, 3D technology, applied in medical science, prosthesis, etc., can solve the problem of no patterned collagen preparation method, and achieve the effect of low cost and simple process

Pending Publication Date: 2019-06-04
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are relatively few studies on the construction of micropatterns on collagen films, and there is no simple and feasible preparation method for patterned collagen

Method used

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  • Collagen film with 3D micro-patterns, and preparation method and application thereof
  • Collagen film with 3D micro-patterns, and preparation method and application thereof
  • Collagen film with 3D micro-patterns, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Use CAD software to draw the top view of the 3D micro-groove pattern, the groove pitch is 50 μm, the groove width is 25 μm, and a mask is made, and the mask is used to prepare 3D micro-grooves on the silicon wafer by photolithography pattern, and then cast PDMS prepolymer on the silicon wafer, and obtain a PMDS substrate with a 3D micro-groove pattern after cross-linking and curing.

[0039] (2) At 4°C, weigh 2.63g of collagen and soak in 400ml of dilute hydrochloric acid with a concentration of 0.01mol / L for 48h. Then, the solution was stirred with a magnetic stirrer. The stirring time was about 48 hours, and the rotation speed of the stirrer was 200-400 rpm. During the stirring process, water was added twice until the volume of the solution was 1000 ml. Dissolve 0.43g of EDC and 0.43g of NHS in 30ml of deionized water respectively, then add the obtained EDC solution and NHS solution into the obtained collagen solution successively, and continue stirring for 3 days...

Embodiment 2

[0043] (1) Use CAD software to draw the top view of the 3D micro-groove pattern, the groove pitch is 50 μm, the groove width is 50 μm, and a mask is made, and the mask is used to prepare 3D micro-grooves on the silicon wafer by photolithography pattern, and then cast PDMS prepolymer on the silicon wafer, and obtain a PMDS substrate with a 3D micro-groove pattern after cross-linking and curing.

[0044] (2) At 4°C, weigh 2.53g of collagen and soak in 400ml of dilute hydrochloric acid with a concentration of 0.01mol / L for 48h. Then, the solution was stirred with a magnetic stirrer. The stirring time was about 48 hours, and the rotation speed of the stirrer was 200-400 rpm. During the stirring process, water was added twice until the volume of the solution was 1000 ml. 0.43g of EDC and 0.43g of NHS were dissolved in 30ml of deionized water respectively, and then the obtained EDC solution and NHS solution were successively added to the obtained collagen solution, and the cross-lin...

Embodiment 3

[0048] (1) Use CAD software to draw the top view of the 3D micro-groove pattern, the groove pitch is 50 μm, the groove width is 100 μm, and a mask is made, and the mask is used to prepare 3D micro-grooves on the silicon wafer by photolithography pattern, and then cast PDMS prepolymer on the silicon wafer, and obtain a PMDS substrate with a 3D micro-groove pattern after cross-linking and curing.

[0049](2) At 4°C, weigh 2.53g of collagen and soak in 400ml of dilute hydrochloric acid with a concentration of 0.01mol / L for 48h. Then, the solution was stirred with a magnetic stirrer. The stirring time was about 48 hours, and the rotation speed of the stirrer was 200-400 rpm. During the stirring process, water was added twice until the volume of the solution was 1000 ml. Dissolve 0.43g of EDC and 0.43g of NHS in 30ml of deionized water respectively, then add the obtained EDC solution and NHS solution to the collagen solution obtained in step (2) successively, and continue stirring ...

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Abstract

The invention belongs to the field of biomedical materials, and discloses a collagen film with 3D micro-patterns as well as a preparation method and application thereof. The collagen film with the 3Dmicro-patterns is prepared by the following steps: forming 3D micro-patterns on a silicon wafer by adopting a photolithography technology by using a mask with 3D micro-patterns; pouring PDMS prepolymers onto the silicon wafer so as to be subjected to cross-linking and curing, thereby obtaining a PDMS base with the 3D micro-patterns; dissolving collagen in an acidic solution, adding water, and carrying out uniform stirring so as to obtain a collagen solution; adding a cross-linking agent into the collagen solution so as to be subjected to cross-linking, thereby obtaining cross-linking reactionproducts; and then, removing bubbles from the cross-linking reaction products, pouring the bubble-removed cross-linking reaction products onto the PDMS base, carrying out air-drying so as to have a film formed, and carrying out stripping so as to obtain the collagen film with the 3D micro-patterns. The preparation technology of the collagen film with the 3D micro-patterns is simple in processes and relatively low in cost; and the prepared film combines advantages of collagen and patterned surface, thereby having good physicochemical properties and biocompatibility. The collagen film with the 3D micro-patterns can be used in corneal tissue engineering so as to induce corneal tissue regeneration; moreover, the collagen film with the 3D micro-patterns is capable of performing regulation on corneal cell behavior, thereby forming a structure similar to native tissues.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a collagen film with 3D micropatterns and a preparation method and application thereof. Background technique [0002] Collagen is a kind of water-insoluble fibrin. It is one of the main components of the extracellular matrix. It plays a key role in maintaining the normal survival of cells and various physiological activities. It exists in large quantities in many tissues in animals. Therefore, when collagen is implanted into the human body as a biological material, it has good biocompatibility and biodegradability, and is not easy to cause immune rejection with the human body. Collagen has high tensile strength and low ductility, strong hydrophilicity, good water permeability and air permeability, so it is widely used in the field of biomedicine. [0003] Surface patterning refers to the construction of different topological patterns on the surface of biomaterials...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/50
Inventor 任力熊思佳高会场覃岚凤
Owner SOUTH CHINA UNIV OF TECH
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