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Composite fluorescent nanoprobe preparation method and in vivo application thereof

A nano-probe and composite fluorescence technology, applied in the research field of fluorescent nano-probes, can solve problems such as qualitative and quantitative analysis, and achieve excellent selective recognition of target toxins, good low toxicity, stable structure and low toxicity Effect

Inactive Publication Date: 2019-05-21
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the above detection methods are mainly aimed at the analysis of in vitro samples, and cannot yet achieve qualitative and quantitative analysis of the distribution and metabolism of food-borne harmful substances in the human body.

Method used

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  • Composite fluorescent nanoprobe preparation method and in vivo application thereof
  • Composite fluorescent nanoprobe preparation method and in vivo application thereof
  • Composite fluorescent nanoprobe preparation method and in vivo application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Preparation method and in vivo application of a composite fluorescent nanoprobe

[0037] (1) A long afterglow-copper sulfide composite nano-fluorescent probe that can specifically recognize aflatoxin was prepared by DNA hybridization reaction.

[0038] Preparation of long afterglow nanomaterials: Accurately weigh 1.4874g of Zn(NO 3 ) 2 ·6H 2 O solid was put into a 50mL round bottom flask, and 10mL Ga(NO 3 ) 3 solution (0.6M), stirring, until Zn(NO 3 ) 2 After fully dissolved, add 300 μL Cr(NO 3 ) 3 solution (0.1M), 1500μL Yb(NO 3 ) 3 solution (0.1M), 150μL Er(NO 3 ) 3 Solution (0.1M) and 10mL ammonium germanate solution (0.1M) were mixed and stirred, and the pH was adjusted to 7.5 with tert-butylamine for co-precipitation, 2mL oleic acid and 15mL toluene were added, and the mixture was uniformly mixed by ultrasonic treatment. Continue magnetic stirring for 2h, and ultrasonic treatment After 30 minutes to make it mix well, the formed white emulsion was placed...

Embodiment 2

[0045] A method for preparing a composite fluorescent nanoprobe and its in vivo application, the steps and methods are basically the same as in Example 1, the difference is that the biotoxin is Aspergillus versicolor, and the corresponding aptamer is an adaptation of Aspergillus versicolor Body sequence, the mice used for biological imaging are mice poisoned by Aspergillus versicolor.

Embodiment 3

[0047] A method for preparing a composite fluorescent nanoprobe and its application in vivo, the steps and methods are basically the same as in Example 1, the difference is that the biotoxin is ochratoxin, and the corresponding aptamer is the aptamer sequence of ochratoxin , the mice used for bioimaging were ochratoxin-poisoned mice.

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Abstract

The invention relates to a fluorescent nanoprobe preparation method and in vivo application. The fluorescent nanoprobe preparation method comprises the following steps: (a) with the help of a DNA hybridization reaction, a long afterglow-copper sulfide composite fluorescent nanoprobe capable of specifically recognizing a target biotoxin is prepared; and (b) selective recognition is carried out through an adapter, and in vivo targeting recognition and in vivo fluorescent imaging monitoring for the target biotoxin by the nanoprobe are realized. The method and the application have the beneficial effects that the prepared long afterglow-copper sulfide composite nanoprobe has excellent selective target toxin recognition ability, the characteristics of ultra-strong near infrared luminescence, ultra-long afterglow lifetime, excellent biocompatibility and structural stability, good particle size uniformity and low toxicity of the long afterglow luminescent nanomaterial and the characteristics of simple synthesis, stable structure and low toxicity of copper sulfide nanoparticles are integrated, and in vivo targeted fluorescence imaging and in vivo tracing are facilitated.

Description

technical field [0001] The invention belongs to the research field of fluorescent nano-probes, and relates to a preparation method of a composite fluorescent nano-probe and its application in vivo. Background technique [0002] Food safety is currently a hot issue of global concern, and it is becoming more and more serious with economic growth and population increase. Food safety is an interdisciplinary field that discusses ensuring food hygiene and food safety during food processing, storage, and sales, reducing hidden dangers of disease, and preventing food poisoning. At present, food safety has become a major threat to human health, and it is also a hot issue of general concern to the whole society. In recent years, with the rapid growth of my country's food supply and consumption, major food safety incidents have occurred frequently, and food safety issues have become more and more prominent. Due to the chemical pollution of a series of food raw materials, the applicat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 刘敬民王硕
Owner NANKAI UNIV
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