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Preparation method of inonotus obliquus polysaccharide chelated zinc

A technology of Inonotus obliquus polysaccharides and Inonotus obliquus, which is applied in the field of chelation, can solve the problems that no system has been formed, and the research on IOP and metal chelation has not been reported, so as to achieve high chelation rate and enhanced scavenging effect , good cleaning effect

Inactive Publication Date: 2019-05-21
CHANGSHU INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, the report of polysaccharide zinc (Ⅱ) chelation has not yet formed a system, and the research on IOP and metal chelation has not been seen.

Method used

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  • Preparation method of inonotus obliquus polysaccharide chelated zinc
  • Preparation method of inonotus obliquus polysaccharide chelated zinc
  • Preparation method of inonotus obliquus polysaccharide chelated zinc

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] (1) Select Inonotus obliquus, pulverize the Inonotus obliquus with a pulverizer, and pass through a 200-mesh sieve to obtain a powder with a relatively uniform particle size, which is dried for later use.

[0046] (2) Weigh 100 g of polysaccharide, and extract it in a water bath at a temperature of 83° C. for 2.2 hours according to a liquid-to-material ratio of 33.3:1.

[0047] (3) After the extraction is completed, it is cooled, and centrifuged at a high speed for 20 minutes by a centrifuge at a speed of 4000 r / min.

[0048] (4) Take the supernatant, concentrate the supernatant in vacuum to 1 / 3 volume of the original volume, add 1 / 4 volume of petroleum ether degreasing to the concentrated solution to degrease.

[0049] (5) Use the Sevage method to remove protein, continue rotary evaporation and concentration to remove the organic solvent, and add 4 times the volume of absolute ethanol to the obtained concentrated solution.

[0050] (6) After alcohol precipitation at 4...

Embodiment 2

[0055] (1) Select Inonotus obliquus, pulverize the Inonotus obliquus with a pulverizer, and pass through a 200-mesh sieve to obtain a powder with a relatively uniform particle size, which is dried for later use.

[0056] (2) Weigh 100 g of polysaccharide, and extract it in a water bath at a temperature of 83°C for 2 hours at a liquid-to-material ratio of 30:1.

[0057] (3) After the extraction is completed, it is cooled, and centrifuged at a high speed for 18 minutes by a centrifuge at a speed of 3500 r / min.

[0058] (4) Take the supernatant, concentrate the supernatant in vacuum to 1 / 3 volume of the original volume, add 1 / 4 volume of petroleum ether degreasing to the concentrated solution to degrease.

[0059] (5) Use the Sevage method to remove protein, continue rotary evaporation and concentration to remove the organic solvent, and add 4 times the volume of absolute ethanol to the obtained concentrated solution.

[0060] (6) After alcohol precipitation at 4°C for 24 hours,...

Embodiment 3

[0065] (1) Select Inonotus obliquus, pulverize the Inonotus obliquus with a pulverizer, and pass through a 200-mesh sieve to obtain a powder with a relatively uniform particle size, which is dried for later use.

[0066] (2) Weigh 100 g of polysaccharide, and extract it in a water bath at a temperature of 83° C. for 4 hours at a liquid-to-solid ratio of 35:1.

[0067] (3) After the extraction is completed, it is cooled, and centrifuged at a high speed for 25 minutes by a centrifuge at a speed of 4000 r / min.

[0068] (4) Take the supernatant, concentrate the supernatant in vacuum to 1 / 3 volume of the original volume, add 1 / 4 volume of petroleum ether degreasing to the concentrated solution to degrease.

[0069] (5) Use the Sevage method to remove protein, continue rotary evaporation and concentration to remove the organic solvent, and add 4 times the volume of absolute ethanol to the obtained concentrated solution.

[0070] (6) After alcohol precipitation at 4°C for 24 hours, ...

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Abstract

The invention discloses a preparation method of inonotus obliquus polysaccharide chelated zinc, which comprises the following steps:1) selecting inonotus obliquus by a pulverizer; 2) weighing the polysaccharide, and extracting in a water bath; 3) cooling and centrifuging; 4) taking supernatant, concentrating, and degreasing; 5) removing protein, removing organic solvent, and adding absolute ethylalcohol; 6) precipitating with alcohol, centrifuging, and freeze-drying to obtain inonotus obliquus polysaccharide; 7) weighing the inonotus obliquus polysaccharide and zinc sulfate heptahydrate in acontainer, wherein the mass ratio of the inonotus obliquus polysaccharide to zinc ions is 4-6:1, the initial concentration of the zinc ions is 4-6; magnetic stirring at room temperature for 6-10h at the stirring speed of 120-180r / min; 8) adding anhydrous ethanol, carrying out alcohol precipitation and centrifugation, taking a precipitate, and lyophilizing to obtain the inonotus obliquus polysaccharide chelated zinc; the chelation rate of the method is high, and the oxidation resistance of the obtained Zn-IOP chelate is remarkably increased.

Description

technical field [0001] The invention relates to a preparation method, in particular to a preparation method of polysaccharide chelated zinc from Inonotus obliquus, belonging to the technical field of chelation. Background technique [0002] Inonotus obliquus is a precious medicinal fungus. Its fruiting body is mainly tumor-shaped, and it is widely parasitic on the trunk or bark of birch, silver birch, alder, elm, etc. The body has strong cold resistance. The northern part of North America, Finland, Poland, Siberia in Russia, parts of the Far East and the Kamchatka Peninsula, Heilongjiang and Changbai Mountains in Jilin Province in China, and Hokkaido in Japan are the main production areas of Inonotus obliquus. Since the 16th and 17th centuries, in Russia, Poland, Japan and other places, Inonotus obliquus, as a precious medicinal fungus, has been widely used in the prevention of various difficult and miscellaneous diseases, such as various liver cancer, gastric cancer, intes...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 陈义勇薛依婷张彤陆婷婷赵文苏乐乐黎英薛美云宫浩兵
Owner CHANGSHU INSTITUTE OF TECHNOLOGY
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