Method for rapidly producing full-female loaches on large scale without using sex hormones

Abstract

The invention discloses a method for rapidly producing full-female loaches on a large scale without using sex hormones, which comprises the following steps of: (1) artificial propagation and hatching:selecting female loaches and male loaches with gonad, wherein the body surfaces of the parents have no trauma, no parasitic attachment, no appearance deformity and anaesthesia is carried out before artificial spawning, artificial insemination is carried out after the an oxytocic is injected, fertilized eggs are naturally stuck on a mesh, and are transferred into a plastic box for artificial incubation; (2) high-temperature conductivity reversal: gradually raising the water temperature of the culture by using a heating rod according to a once-a-day speed, continuously carrying out high-temperature treatment, and then cooling and culturing; (3) testing mating and group expanding: breeding the remaining male fish to sexual maturity, dividing the offspring generated by each group of male andfemale fish into two parts for independent breeding, removing female individuals in the group, and breeding the remaining XX type male fish to sexual maturity; (4) full-female loach production. The method is easy and simple to operate, can quickly produce the full-female loaches with high growth speed, and does not use sex steroid hormones in the whole process.

Description

technical field [0001] The invention belongs to the technical field of fish parthenogenesis technology or fish artificial sex control, and more specifically relates to a method for rapid and large-scale production of whole female loach without using sex hormones. Background technique [0002] The national output of loach (Misgurnus anguillicaudatus) in 2017 was 390,000 tons, an increase of about 6% compared with that in 2016. Statistical Yearbook). my country's loach production accounts for 99.7% of the world's production. In 2016, the world's total output value was 943 million US dollars, ranking 34th in the world's freshwater fish production value (Fisheries and Aquaculture Software, 2018, FAO). It is an important economic fish in the world and in my country. [0003] Under the same breeding conditions or experimental conditions, male and female loach showed obvious growth differences, and females grew 50% to 150% faster than males. All-female loach breeding can certainl...

AI Technical Summary

Problems solved by technology

[0005] Although individuals with sexual reversal induced by sex steroid hormones are only used as parents and will not enter the consumer market, the use of sex hormones (whether directly soaking fry, soaking live bait for fry, or feeding with mixed feed) will inevitably lead to the residue or metabolism of some sex hormones. The product enters the aquaculture water body or natural water body (pond, lake, reservoir, etc.), and has a negative impact on the aquaculture fish or the natural ecosystem

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