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Automated device and method for detecting mycotoxins

An automated equipment and mycotoxin technology, applied in the field of analytical chemistry detection, can solve the problems of limiting food safety control timeliness, complex operation, high labor intensity, etc., and achieve the effects of reducing solvent replacement time, high detection sensitivity, and improving work efficiency

Active Publication Date: 2019-05-10
依利特(苏州)分析仪器有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Not only is the operation complex, time-consuming, labor-intensive, large errors, but also low detection accuracy
At the same time, the detection and analysis personnel have more contact with mycotoxins during the whole operation process, which poses a serious threat to the health of the detection and analysis personnel
[0004] In addition, the main problem is that the basic source of fluorescent detection devices for detecting mycotoxins is imported. The detection of fluorescent substances in the domestic market is mostly special-purpose fluorescent detectors, such as the special detection analyzers for aflatoxin on the market, etc.
The main problem of this special-purpose detection device is that it has a single function, which can only meet the detection of one substance, and cannot realize the simultaneous detection of multiple toxins, and its sensitivity is low.
[0005] The use of imported fluorescence detection devices has the factors of expensive experimental platform equipment, large volume, and high maintenance costs, which limit the timeliness of food safety control in my country

Method used

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  • Automated device and method for detecting mycotoxins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: A kind of automatic equipment for detecting mycotoxin

[0039] (1) Composition of an automated device for detecting mycotoxins

[0040] like figure 1 As shown, an automated device for detecting mycotoxins includes: a control circuit system, a sample pretreatment system, a fluorescence detection system and a data processing system.

[0041] The control circuit system includes a control circuit X and a power module Y. Sample pretreatment system includes low-pressure quaternary mixer A, pretreatment solvent storage tanks A1-A4, first high-pressure constant-flow pump B1, ternary switching valve C, first syringe pump D1, second syringe pump D2, homogenizer E. Reactor F, two-position six-way switching valve G, trapping column I, waste liquid bottle J, second high-pressure constant-flow pump B2 and elution solvent storage tank A5, sample bottle K, mobile phase storage tank A6, the second Three high-pressure constant-flow pumps B3, autosampler H and chromatogra...

Embodiment 2

[0049] Embodiment 2 adopts the automated equipment of the present invention to be used for the detection of aflatoxin in the sample to be tested

[0050] Put the required solvents in the pretreatment solvent storage tank A1-A4, elution solvent storage tank A5 and mobile phase storage tank A6, A1: methanol aqueous solution; A2: water; A5: methanol; A6: mobile phase.

[0051] The specific operation is as follows:

[0052] 1. Weigh 25g of the sample to be tested and place it in the homogenizer E, draw 125mL of the solution in A1 into the homogenizer E through the first high-pressure constant-flow pump B1 and the three-way switching valve C, and stir for 2 minutes;

[0053] 2. Draw 15mL of the sample extract in the homogenizer E through the first syringe pump D1 and filter it in the reactor F, then quantitatively suck in 30mL of the solution in A2 through the first high-pressure constant-flow pump B1 and the three-way switching valve C to react In reactor F, after a period of rea...

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Abstract

The invention belongs to the field of analytical chemical detection, and specifically relates to an automated device and method for detecting mycotoxins. The automated device comprises a control circuit system, a sample pre-processing system, a fluorescence detection system, and a data processing system. The control circuit system is used for controlling transmission of electrical signals betweenvarious systems in devices; the sample pre-processing system is connected with the fluorescence detection system through a pipeline, and the pre-processed sample is conveyed to the fluorescence detection system for detection; and the data processing system is connected with the fluorescence detection system and receives a fluorescent signal detected by the fluorescent detection system, and mycotoxins concentration of the to-be-tested sample is obtained after the fluorescent signal is subjected to data processing. According to the automated device for detecting the mycotoxins, the fluorescencedetection system is provided with a multi-wavelength light source automatic switching optical system and a multi-wavelength LED light source disk, and an ultraviolet LED light source can be automatically switched as needed to generate excitation light of different wavelengths, and the simultaneous detection of various mycotoxins can be satisfied.

Description

technical field [0001] The invention relates to the field of analytical chemistry detection, in particular to a device and method for detecting mycotoxins in samples. Background technique [0002] Mycotoxins are secondary metabolites produced by fungi. Mycotoxins mainly exist in grain, feed and food. Improper storage of grain, insufficient drying, or high temperature or humidity during storage and transportation will cause the fungus contaminated on grain and feed to grow rapidly; long-soaked fungus may It will deteriorate and produce similar biological toxins, or breed pathogenic microorganisms such as bacteria and fungi; unwashed chopsticks are very easy to hide starch in the seams of chopsticks, mildew, and produce aflatoxin; poor-quality sesame paste, spoiled rice, hair Bitter nuts, etc., all contain mycotoxins. In fact, mycotoxins have been hidden around us all the time, threatening human health at all times. [0003] At present, most of the detection methods for myc...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N21/01
Inventor 王文晶周新颖韩雪封娇王丰琳唐涛于德秀李彤
Owner 依利特(苏州)分析仪器有限公司
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