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Method for preparing hydrogel wound dressing through low-temperature biological 3D printing technology

A 3D printing and hydrogel technology, applied in the field of medical products, can solve the problems of poor regeneration of granulation tissue on exposed wounds, rapid loss of active ingredients, and aggravated nursing tasks, so as to facilitate long-term storage and transportation, and accelerate wound regeneration and repair Speed, the effect of promoting the regeneration of granulation tissue

Active Publication Date: 2019-05-10
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This kind of repair bracket is mainly aimed at bone repair, and has a poor regeneration effect on the granulation tissue of exposed wounds, and the effective ingredients are lost quickly, so the interval for replacing the bracket is short, which increases the treatment cost and aggravates the nursing tasks.

Method used

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  • Method for preparing hydrogel wound dressing through low-temperature biological 3D printing technology
  • Method for preparing hydrogel wound dressing through low-temperature biological 3D printing technology
  • Method for preparing hydrogel wound dressing through low-temperature biological 3D printing technology

Examples

Experimental program
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Effect test

Embodiment 1

[0031] A method for preparing a low-temperature bio-3D printed hydrogel wound dressing: including (1) dissolving 8% sodium alginate (SA) in deionized water, absorbing water to swell for 6 hours at room temperature, and then fully mechanically stirring for 30 minutes; Dissolve gelatin (GA) with a mass fraction of 8% in deionized water, and stir electromagnetically at 40°C for 30 minutes; finally, blend the sodium alginate solution and gelatin solution at a mass ratio of 1:1, and stir thoroughly at 40°C until a uniform A blend solution containing 4% SA and 4% GA.

[0032] (2) Combination of biological factors and hydrogel matrix materials: Add 1ml of 20μg / ml VEGF solution into 9ml of 4%SA / 4%GA blend solution, stir at 37 degrees Celsius for 5 minutes, and finally get 2μg / ml VEGF loaded Hydrogel matrix material; add 1ml 2mg / ml LL-37 solution to 9ml 4%SA / 4%GA blend solution, stir at 37°C for 5 minutes, and finally get a hydrogel matrix loaded with 200μg / ml LL-37 Material.

[0033...

Embodiment 2

[0036] A method for preparing a low-temperature bio-3D printed hydrogel wound dressing, comprising: (1) dissolving 2% sodium alginate (SA) in deionized water, absorbing water and swelling for 6 hours at room temperature, and then fully mechanically stirring for 30 minutes ; Dissolve gelatin (GA) with a mass fraction of 15% in deionized water, and stir electromagnetically at 40 degrees Celsius for 30 minutes; finally, blend the sodium alginate solution and gelatin solution at a mass ratio of 5:1, and stir thoroughly at 40 degrees Celsius until A uniform blend solution containing 4% SA and 4% GA.

[0037] (2) Combination of biological factors and hydrogel matrix materials: Add 1ml of 20μg / ml VEGF solution into 9ml of 4%SA / 4%GA blend solution, stir at 37 degrees Celsius for 5 minutes, and finally get 2μg / ml VEGF loaded Hydrogel matrix material; add 1ml 2mg / ml LL-37 solution to 9ml 4%SA / 4%GA blend solution, stir at 37°C for 5 minutes, and finally get a hydrogel matrix loaded with ...

Embodiment 3

[0041] A method for preparing a low-temperature bio-3D printed hydrogel wound dressing, comprising: (1) dissolving 2% sodium alginate (SA) in deionized water, absorbing water and swelling for 6 hours at room temperature, and then fully mechanically stirring for 30 minutes ; Dissolve gelatin (GA) with a mass fraction of 15% in deionized water, and stir electromagnetically at 40 degrees Celsius for 30 minutes; finally, blend the sodium alginate solution and gelatin solution at a mass ratio of 5:1, and stir thoroughly at 40 degrees Celsius until A uniform blend solution containing 4% SA and 4% GA.

[0042] (2) Combination of biological factors and hydrogel matrix materials: Add 1ml of 20μg / ml VEGF solution into 9ml of 4%SA / 4%GA blend solution, stir at 37 degrees Celsius for 5 minutes, and finally get 2μg / ml VEGF loaded Hydrogel matrix material; add 1ml 2mg / ml LL-37 solution to 9ml 4%SA / 4%GA blend solution, stir at 37°C for 5 minutes, and finally get a hydrogel matrix loaded with ...

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Abstract

The invention provides a method for preparing a hydrogel wound dressing through a low-temperature biological 3D printing technology. The method comprises the following steps that 1, a sodium alga acid / gelatin composite hydrogel medium material is prepared; 2, a vascular endothelial growth factor (VEGF) and antibacterial peptide LL-37 are loaded into the medium material separately; 3, through the multi-spray-nozzle low-temperature biological 3D printing technology, the wound dressing of a three-dimensional structure is prepared; 4, through a curing technology and a freezing drying technology, the wound dressing of a stable three-dimensional structure is obtained. The method has the advantages that the functions of promoting good granulation tissue regeneration and resisting bacteria are achieved; the controllable space and concentration distribution of biological factors are achieved, the whole dressing preparation process is mild, and the activity of the biological factors is guaranteed; the final product is in a dried and stable state and is easy to store and transport for a long time.

Description

technical field [0001] The invention relates to the field of medical products, in particular to a method for preparing a low-temperature biological 3D printed hydrogel wound dressing. Background technique [0002] A large area of ​​severe skin and soft tissue trauma caused by trauma, burns, diseases, etc. is a difficult clinical problem. Skin flap technology is currently the main clinical treatment. However, the application of flap technology is limited due to lack of donor sites, high cost, additional surgical risks, and surgical pain. In recent years, the adjunct or even replacement of skin flaps with artificial wound dressings has become a promising wound treatment strategy. [0003] At present, there are also some 3D printing dressing products, such as a 3D bioprinted medical dressing and its preparation method disclosed by the patent No. The prepared bone repair scaffold has a single function and does not have antibacterial properties, which often leads to its suscep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L26/00C08J3/075C08J3/24B33Y70/00C08L5/04C08L89/00
Inventor 熊蠡茗石磊刘静王文天胡益强张祥林
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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