Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Separation and purification method of L-valine

A technology for separation and purification of valine, applied in the field of separation and purification of L-valine, can solve the problems of complex separation and purification process, large amount of production wastewater, high environmental pollution, etc., and achieve simplification of separation and purification process and optimization of separation and purification process , the effect of reducing production costs

Active Publication Date: 2019-04-26
内蒙古拜克生物有限公司
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The precipitation method is based on the specific combination of the precipitant and L-valine to form a precipitate, and then separate and extract. This method has the advantages of high yield and easy operation, but there is a technical problem of high environmental pollution; less, but the membrane filtration method can only remove a large amount of inorganic salt ions and macromolecular impurities, and the amino acids similar to L-valine in the fermentation broth cannot be eradicated, and the obtained L-valine has high impurities; The ion exchange method is to adjust the pH of the fermented liquid from which the bacteria have been removed to be acidic, use a strong acidic cation exchange resin to separate L-valine and impurity amino acids, and finally separate L-valine by elution with ammonia water
If the purity of L-valine is improved, it is necessary to adopt multi-stage ion exchange columns for serial extraction, which will lead to a large amount of production wastewater in the production process, and the extraction yield by ion exchange method is also low
[0005] CN105274182A discloses a process for efficiently extracting L-valine from fermentation broth, comprising the following steps: 1) fermentation, 2) microfiltration membrane filtration: 3) evaporation concentration, 4) crystallization, 5) dissolution, 6) ionization Exchange, 7) ultrafiltration membrane filtration, 8) vacuum concentration and 9) centrifugal drying, this invention adopts the combined use of membrane filtration method and ion exchange method, which improves the extraction yield and purity of L-valine, but this invention does not The crystallization effect was not paid attention to, resulting in an unsatisfactory yield of L-valine, only about 68%
Although the present invention can obtain L-valine with higher purity, because the solubility of valine in pure water does not change much with temperature, the present invention adopts cooling crystallization, and a large amount of solutes are still dissolved in the solvent when the crystallization is finished. In the process, the yield of crystallized product is low, which leads to the low actual yield of L-valine product, which cannot realize industrialization
[0007] At present, the separation and purification technology of L-valine in my country is backward, and a large amount of solutes are still dissolved in the solvent during the crystallization process, resulting in low yield of crystallized products, which greatly affects the output of L-valine. At the same time, the separation and purification process is complicated , the cost is high, resulting in the poor market competitiveness of my country's domestic L-valine, and it is impossible to achieve large-scale production

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] According to the mass ratio of 1000:2, add a flocculant composed of β-cyclodextrin and chitosan with a mass ratio of 1:5 to the valine fermentation broth, and use it at 25°C and a frequency of 22kHz After ultrasonic treatment for 2 minutes, a double-diaphragm plate-and-frame filter press is used for pressure filtration to obtain a press-filtrate;

[0073] passing the press filtrate through a microfiltration membrane with a molecular weight cut-off of 5000MW to obtain a filtrate; concentrating the filtrate to 1 / 3 of the volume of the original filtrate to obtain a concentrate 1;

[0074] According to the volume ratio of 1:0.5, add absolute ethanol with a temperature of -10°C to the concentrated solution 1, and use ultrasonic treatment for 5 minutes at 5°C and a frequency of 22kHz, and then centrifuge at 8000rpm for 5 minutes to obtain L - Crude valine;

[0075] Adding a 1% sodium chloride solution by mass percentage to the L-valine crude product at a mass ratio of 1:1 to...

Embodiment 2

[0079] According to the mass ratio of 1000:5, add a flocculant composed of β-cyclodextrin and chitosan with a mass ratio of 1:10 to the valine fermentation broth, and use it at 30°C and a frequency of 28kHz After ultrasonic treatment for 10 minutes, press filtration with a double-diaphragm plate-and-frame filter press to obtain a press-filtrate;

[0080] passing the press filtrate through a microfiltration membrane with a molecular weight cut-off of 5000MW to obtain a filtrate; concentrating the filtrate to 1 / 3 of the volume of the original filtrate to obtain a concentrate 1;

[0081] According to the volume ratio of 1:1, add absolute ethanol with a temperature of -15°C to the concentrated solution 1, and use ultrasonic treatment for 10 minutes at 10°C and a frequency of 28 kHz, and then centrifuge at 8000 rpm for 15 minutes to obtain L - Crude valine;

[0082] Adding a 0.5% sodium chloride solution by mass percentage to the L-valine crude product at a mass ratio of 1:1 to ob...

Embodiment 3

[0086] According to the mass ratio of 1000:2.5, add a flocculant composed of β-cyclodextrin and chitosan with a mass ratio of 1:7.5 to the valine fermentation broth, and use it at 27°C and a frequency of 25kHz After ultrasonic treatment for 5 minutes, a double-diaphragm plate-and-frame filter press is used for pressure filtration to obtain a press-filtrate;

[0087] passing the press filtrate through a microfiltration membrane with a molecular weight cut-off of 5000MW to obtain a filtrate; concentrating the filtrate to 1 / 3 of the volume of the original filtrate to obtain a concentrate 1;

[0088] According to the volume ratio of 1:0.8, add absolute ethanol with a temperature of -12°C to the concentrated solution 1, use ultrasonic treatment for 8 minutes at 8°C and a frequency of 25 kHz, and then centrifuge at 8000 rpm for 10 minutes to obtain L - Crude valine;

[0089] Adding a 0.65% sodium chloride solution by mass percentage to the L-valine crude product at a mass ratio of ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a separation and purification method of L-valine. The method comprises the following steps: S1, filter-pressing, S2, micro-filtration membrane filtering; S3, concentrating andcrystallizing; S4, dissolving; S5, ion exchanging; S6, ultra-filtering and concentrating; and S7, crystallizing and drying. The separation and purification method comprises the advantages that the yield of the L-valine is improved, and the content of isoleucine in the L-valine is reduced, thereby providing technical support for the industrial development of the L-valine of our country.

Description

technical field [0001] The invention relates to the field of microbial fermentation, and relates to a method for separating and purifying L-valine. Background technique [0002] L-valine (L-val), scientific name 2-amino-3-methylbutyric acid, is one of the eight essential amino acids for the human body. It is collectively called branched-chain amino acids together with L-leucine and L-isoleucine. It is widely used in medicine, seasoning, cosmetics, nutritional additives, feed additives, etc. [0003] The production methods of L-valine mainly include extraction method, synthesis method and fermentation method. Among them, microbial fermentation method has the advantages of low raw material cost, mild reaction conditions and easy realization of large-scale production, and has become a widely used production method. [0004] Separation and purification is an important process in the industrial production of L-valine. Usually, the cost of separation and purification accounts for...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07C227/40C07C229/08
CPCC07C227/40C07C229/08
Inventor 王奇马光辉钟迎东叶朋宫颖章
Owner 内蒙古拜克生物有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products