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Composite bone repairing material and preparation method thereof

A technology for bone repair and collagen, applied in the fields of medical science, prosthesis, tissue regeneration, etc., can solve the problems of inoperable repair parts, easy inactivation of growth factors, no obvious effect, etc., to increase the application field and scope, raw materials Ease of availability and the effect of increasing the scope of application

Active Publication Date: 2019-04-26
河北科硕生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing single-component collagen-based bone materials can only promote the proliferation of osteoblasts and have no obvious effect on the vascularization process; while growth factors are easily inactivated and difficult to preserve, the current slow-release technology still cannot make Its long-term and efficient action on the repair site

Method used

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  • Composite bone repairing material and preparation method thereof
  • Composite bone repairing material and preparation method thereof
  • Composite bone repairing material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0052] The preparation method includes the following steps:

[0053] (1) Weigh type I and type III collagen, disperse them in an acetic acid solution according to a certain weight ratio, stir and mix until the system is uniform; the role of acetic acid is to dissolve the collagen;

[0054] (2) Add (NH) to the system obtained in step (1) 4 ) 2 HPO 4 After stirring for several hours, adjust the pH with aqueous ammonia solution, adjust the environment required for the reaction, and slowly add Ca(NO 3 ) 2 Continue the reaction, filter after the reaction, and repeatedly wash the precipitate with water to prepare a bionic bone material;

[0055] (NH 4 ) 2 HPO 4 The role is phosphorylation, Ca(NO 3 ) 2 The function is to synthesize hydroxyapatite in situ after adding;

[0056] Further, the preparation method further includes: (3) placing the precipitate obtained in step (2) in a mold for forming, pre-freezing in an ultra-low temperature refrigerator at -80°C for 24 hours, and then placing it ...

experiment example 1

[0067] Experimental example 1. Preparation method of composite bone repair material of the present invention

[0068] Weigh type I and type III collagen, disperse them in the acetic acid solution at a ratio of 9:1 (W / W), stir and mix until the system is uniform, and add a certain amount of (NH 4 ) 2 HPO 4 After stirring for several hours, adjust the pH to 7.5 with ammonia water, and slowly add Ca(NO 3 ) 2 Continue the reaction for 24 hours, filter after the reaction, and wash the precipitate repeatedly with water, place it in a mold for molding, pre-frozen in an ultra-low temperature refrigerator at -80°C for 24 hours, and then place it in a freeze dryer at -50°C for 24 hours.

[0069] Hydroxyapatite is synthesized in situ, not directly added, adding "(NH 4 ) 2 HPO 4 "And then add "Ca(NO 3 ) 2 "Hydroxyapatite" can be formed under certain pH conditions. The specific reaction formula is as follows: 10Ca(NO E ) 2 -6(NH 4 ) 2 HP 4 →Ca 10 (PO 4 ) 6 (OH) 2

[0070] "(NH 4 ) 2 HPO 4 "And "Ca...

experiment example 2

[0074] Experimental example 2. The effect comparison between the composite collagen bionic bone material of the present invention and the existing bionic bone material

[0075] The present invention uses the animal model of New Zealand white rabbit radius defect, and uses the existing bionic bone material (single collagen + hydroxyapatite) and the composite collagen bionic bone material of the present invention to repair the bone. The results are as follows image 3 As shown, at 3 months postoperatively, the defect area of ​​the control group (A) had a large amount of low-density callus formation, and the defect area of ​​the experimental group (B) was filled with a large amount of new bone, and a bridge was formed between the two broken ends. Medullary cavity recanalization; 6 months after the operation, there was a lot of low-density callus formation in the defect area of ​​the control group (A), but there was no recanalization of the medullary cavity; the experimental group (B) ...

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Abstract

The invention discloses a composite bone repairing material and a preparation method thereof. The composite bone repairing material is compounded by taking hydroxyapatite and two different types of collagen as matrices through an in-situ synthesis method; the ratio of the total collagen to the hydroxyapatite in parts by weight ranges from 1:9 to 1:1; the ratio of type-I collagen to type-III collagen in parts by weight is (85 to 99.99):(0.01 to 15). The composite bone repairing material has the advantages that the different types of collagen are involved in the composite bone repairing material, no foreign matter is introduced, and accordingly, low immunogenicity and high biocompatibility of the collagen can be maintained; different effects of the different types of collagen can be embodiedat the same time, and accordingly, the composite bone repairing material is multifunctional.

Description

Technical field [0001] The invention belongs to the field of biological materials, and specifically relates to a composite bone repair material and a preparation method thereof. Background technique [0002] Bone damage caused by traffic accidents, construction accidents, aging and various other reasons reduces people's quality of life, and severely affects people's normal work and life, making bone transplantation the second only to blood transfusion. However, autologous bone is prone to secondary trauma, nerve damage or infection, which severely restricts the development of bone repair and reconstruction disciplines, and becomes a bottleneck restricting the development of orthopedic treatment disciplines. [0003] In the past two decades, tissue engineering has received attention because of its ability to use engineering and life science methods to develop biological substitutes for repairing, rebuilding or improving tissue function. The development of bone repair materials is a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/46C04B28/34
CPCA61L27/46A61L2430/02C04B28/344C04B2111/00836C04B18/18C04B22/16C04B24/04C04B22/06C04B22/085C08L89/00
Inventor 郭雅楠李志宏王微
Owner 河北科硕生物技术有限公司
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