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Creation of cardiac troponin I immunosensors on basis of flaky copper sulfur molybdate

A cardiac troponin and immune sensor technology, applied in the direction of instruments, scientific instruments, and material electrochemical variables, can solve the problem of low detection accuracy and achieve high sensitivity and specificity detection, enhanced light absorption intensity, and improved sensitivity Effect

Active Publication Date: 2019-04-19
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above two methods have realized the detection of cardiac troponin I, the detection accuracy is not very high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Construction of a Cardiac Troponin I Immunosensor Based on Sheet-Shaped Copper Sulfur Molybdenum

[0035] (1) The ITO conductive glass was ultrasonically cleaned with detergent, acetone, ethanol, 1 mol / L sodium hydroxide solution with a volume ratio of ethanol / ultrapure water of 1:1, and ultrapure water for 0.5 h respectively. Dry in nitrogen atmosphere at ℃ for 140 min;

[0036] (2) Take 10 µL of the graphene / copper molybdenum sulfide solution and drop it on the conductive surface of the ITO conductive glass, and let it dry at room temperature;

[0037] (3) Manganese-doped cadmium sulfide is modified on graphene / flaky copper molybdenum sulfide conductive glass by continuous ion adsorption and reaction to prepare graphene / flaky copper molybdenum sulfur oxide / manganese-doped cadmium sulfide electrode ;

[0038] (4) Immerse the above-mentioned modified electrode in 10 mmol / L thioglycolic acid solution, take it out after 10 min, and then add dropwise 400 mmol / L...

Embodiment 2

[0041] Example 2 Construction of a Cardiac Troponin I Immunosensor Based on Copper Sulfur Molybdenum

[0042] (1) The ITO conductive glass was ultrasonically cleaned with detergent, acetone, ethanol, 1 mol / L sodium hydroxide solution with a volume ratio of ethanol / ultrapure water of 1:1, and ultrapure water for 0.5 h respectively. Dry in nitrogen atmosphere at ℃ for 140 min;

[0043] (2) Take 10 µL of the graphene / copper molybdenum sulfide solution and drop it on the conductive surface of the ITO conductive glass, and let it dry at room temperature;

[0044] (3) Manganese-doped cadmium sulfide is modified on graphene / flaky copper molybdenum sulfide conductive glass by continuous ion adsorption and reaction to prepare graphene / flaky copper molybdenum sulfur oxide / manganese-doped cadmium sulfide electrode ;

[0045] (4) Immerse the above-mentioned modified electrode in 10 mmol / L thioglycolic acid solution, take it out after 10 min, and then add dropwise 400 mmol / L 1-ethyl-(3-d...

Embodiment 3

[0048] Example 3 Construction of a Cardiac Troponin I Immunosensor Based on Copper Sulfur Molybdenum

[0049] (1) The ITO conductive glass was ultrasonically cleaned with detergent, acetone, ethanol, 1 mol / L sodium hydroxide solution with a volume ratio of ethanol / ultrapure water of 1:1, and ultrapure water for 0.5 h respectively. Dry in nitrogen atmosphere at ℃ for 140 min;

[0050] (2) Take 10 µL of the graphene / copper molybdenum sulfide solution and drop it on the conductive surface of the ITO conductive glass, and let it dry at room temperature;

[0051] (3) Manganese-doped cadmium sulfide is modified on graphene / flaky copper molybdenum sulfide conductive glass by continuous ion adsorption and reaction to prepare graphene / flaky copper molybdenum sulfur oxide / manganese-doped cadmium sulfide electrode ;

[0052] (4) Immerse the above-mentioned modified electrode in 10 mmol / L thioglycolic acid solution, take it out after 10 min, and then add dropwise 400 mmol / L 1-ethyl-(3-d...

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PUM

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Abstract

The invention relates to creation of cardiac troponin I immunosensors on the basis of flaky copper sulfur molybdate, and belongs to the technical field of creation of novel sensors. Graphene / the flakycopper sulfur molybdate / manganese-doped cadmium sulfide are used as substrate materials, and ultraviolet-visible light irradiates on the substrate materials to obtain light currents. The creation hasthe advantages that flaky copper sulfur molybdate and manganese-doped cadmium sulfide energy bands in the substrate materials are excellently matched with one another, the electron transport capacitycan be improved by the graphene, and accordingly the photoelectric conversion efficiency can be greatly improved; effects of quenching the light currents can be realized by cardiac troponin I, the to-be-detected cardiac troponin I is different in quantity, and accordingly the degrees of influence on photoelectric signals can be different from one another; the cardiac troponin I can be detected bythe created immunosensors, and the detection limit of the cardiac troponin I immunosensors is 0.18 pg / mL.

Description

technical field [0001] The present invention relates to a preparation method and application of a photoelectrochemical immunosensor of a ternary transition metal sulfide. More specifically, the present invention uses graphene / flaky sulfur molybdenum copper / manganese doped cadmium sulfide as the base material to prepare a The invention discloses a label-free immunosensor for sensitively detecting cardiac troponin I, which belongs to the technical field of new functional materials and biosensing detection. Background technique [0002] Cardiac troponin I is unique to myocardial tissue. When myocardial cell injury occurs, the content of cardiac troponin I will increase rapidly and last for a long time, up to 6 to 10 days. Therefore, cardiac troponin I has become a biomarker for the diagnosis of early myocardial cell injury. [0003] At present, there are many clinical detection methods for cardiac troponin I, including electrochemical method, electrochemiluminescence method, e...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/327G01N33/531
CPCG01N27/30G01N27/3275G01N33/531
Inventor 吴丹池惠彤韩清志池天鹤王欢匡轩魏琴
Owner UNIV OF JINAN
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