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A kind of compound microbial living bacteria preparation and its application in high concentration pig raising wastewater

A technology for compound microorganisms and live bacteria preparations, which is applied in microorganism-based methods, microorganisms, biological water/sewage treatment, etc., can solve the problem that the mixed bacteria fermentation process needs to be optimized, and achieve the effect of efficient removal and wide application prospects.

Active Publication Date: 2020-03-13
北京航天威科环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Our country only carried out relevant laboratory research work in the 1990s. The laboratory has formed a mature strain culture technology. Through the compounding of different strains, the efficiency of sewage treatment can be improved, but the fermentation process of mixed bacteria still needs to be optimized.

Method used

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  • A kind of compound microbial living bacteria preparation and its application in high concentration pig raising wastewater
  • A kind of compound microbial living bacteria preparation and its application in high concentration pig raising wastewater
  • A kind of compound microbial living bacteria preparation and its application in high concentration pig raising wastewater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The isolation of embodiment 1 bacterial strain

[0033] Take 10g of feces from pig farms, put them into a conical flask containing sterilized glass beads and 50mL of normal saline, shake at 30°C and 150r / min for 2h, let it stand for 20min, take 1mL of the supernatant, and wash it continuously with sterilized physiological water. Make a 10-fold gradient dilution, draw 200 μL of suspensions with different dilutions, spread them on MRS solid medium, nutrient broth agar medium, and YPD solid medium respectively, and culture them at 28°C for 28-72 hours to observe the growth of the strains. Take a single colony for culture. According to the shape and growth of the strains, the strains with different shapes were picked for streak culture and stored at low temperature. A total of 40 strains of bacteria were isolated, including 26 strains of bacteria, 8 strains of yeast and 6 strains of lactobacilli.

[0034] Above-mentioned culture medium and formula thereof are as follows: ...

Embodiment 2

[0038] Embodiment 2: Screening and identification of bacterial strains

[0039] 1 Primary screening of strains

[0040] Add 150g of fresh pig manure into a 500mL Erlenmeyer flask with a stopper, inoculate the isolated strains of Example 1 according to an inoculation amount of 5% by volume, and cultivate them in a 30°C incubator for 10 days, and use the sensory method to evaluate the odor level , the results are shown in Table 1. It can be seen from Table 1 that the deodorizing ability of the isolated microorganisms is different. Compared with the control, three strains of bacteria, one strain of lactic acid bacteria and two strains of yeast have better deodorizing ability.

[0041] The odor level is evaluated by the sensory method, and the gas odor level is divided by the 6-level method:

[0042] Level 0: no odor, represented by "-";

[0043] Grade 1: Barely feel the smell, indicated by "+";

[0044] Level 2: weak odor, represented by "++";

[0045] Level 3: Obvious odor,...

Embodiment 3

[0077] The preparation method of composite microbial living bacteria preparation, the steps are as follows:

[0078] (1) Preparation of bacterial suspension

[0079] Use nutrient agar medium to activate and cultivate Bacillus cereus 2-2, and obtain a single colony after 48 hours of activation culture, pick a single colony and inoculate it in a nutrient broth medium test tube, culture it on a shaking table at 25-35°C, 160rpm for 24 hours, and obtain Seed solution: take the seed solution, transfer it to the same medium according to the volume ratio of 2%, and carry out expanded cultivation under the same conditions for 36 hours to obtain the culture bacteria solution; centrifuge the culture bacteria solution at 5000rpm for 20min, discard the supernatant to obtain the bacteria Dilute the cells with 0.9% normal saline according to the ratio of wet cell weight to normal saline volume of 1:50 (g / mL) to obtain a suspension of Bacillus cereus 2-2;

[0080] Use nutrient broth agar med...

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Abstract

The invention relates to a composite microbial viable bacteria preparation and an application thereof in high-concentration pig-raising wastewater. The composite microbial viable bacteria preparationis a bacterial suspension which comprises Bacillus cereus 2-2, Pseudomonas sp. C2-1, Pichia pastoris 2-6 and Lactobacillus zeae 2-20, wherein the viable count of Bacillus cereus 2-2 is greater than orequal to 1.48*1010 CFU / mL, the viable count of Pseudomonas sp. C2-1 is greater than or equal to 2.04*1010 CFU / mL, the viable count of Pichia pastoris 2-6 is greater than or equal to 0.98*1010 CFU / mL,and the viable count of Lactobacillus zeae 2-20 is greater than or equal to 1.78*1010 CFU / mL. The composite microbial viable bacteria preparation is used for treating wastewater containing high-concentration COD and NH3-N, thereby achieving high-efficiency removal of COD and nitrogen.

Description

technical field [0001] The invention relates to a compound microbial living bacteria preparation and its application in high-concentration pig-raising wastewater, belonging to the technical field of high-concentration pig-raising wastewater treatment. Background technique [0002] Livestock and poultry breeding is one of the main causes of water pollution in rural areas of my country. With the rapid development of my country's livestock and poultry industry, the pollution of livestock and poultry wastewater has become increasingly serious, seriously polluting surface water, groundwater, soil and ambient air, directly affecting people's health and normal life. Therefore, how to protect the ecological environment while the livestock and poultry industry develops steadily has become the focus and difficulty of current research. [0003] The purpose of waste treatment in farms is to make them harmless, reduce and recycle them, and meet the environmental acceptability and feasib...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16C02F3/34C12R1/085C12R1/38C12R1/84C12R1/225C02F103/20
CPCC02F3/341C02F3/347C02F3/348C02F2103/20C12N1/16C12N1/20
Inventor 黄玉杰王加宁宋繁永陈贯虹高永超张闻赵庆庆王磊磊郑立稳
Owner 北京航天威科环保科技有限公司
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