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Portable multi-channel spectrophotometer based on smart phone, and method for measuring absorbance

A technology of spectrophotometer and smart phone, which is applied in the field of analysis and detection, can solve the problems of large instrument volume and high power consumption, and achieve the effect of small volume, low power consumption and low cost

Inactive Publication Date: 2019-04-16
ZAOZHUANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In view of the fact that the commercial spectrophotometer described in the prior art has a large volume, high power consumption, and is not suitable for field analysis, and the current situation that the accuracy of the absorbance measurement device based on a smart phone is affected by the fluctuation of the light source intensity, the present invention proposes A portable multi-channel spectrophotometer based on a smart phone and a method for measuring absorbance. The absorbance is no longer affected by the fluctuation of light source intensity, and the wavelength and absorption path of monochromatic light can be switched conveniently.

Method used

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  • Portable multi-channel spectrophotometer based on smart phone, and method for measuring absorbance
  • Portable multi-channel spectrophotometer based on smart phone, and method for measuring absorbance
  • Portable multi-channel spectrophotometer based on smart phone, and method for measuring absorbance

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Embodiment 1

[0064] Such as image 3 As shown, a portable multi-channel spectrophotometer based on a smart phone includes a light-proof cassette 4, a light source 2, a cuvette holder 5, and a measurement and analysis unit 11, and the measurement and analysis unit 11 is an intelligent device equipped with a camera 10 Mobile phone, the described cuvette holder 5 is located between the light source 2 and the measurement and analysis unit 11, so that the camera 10 of the measurement and analysis unit 11 is directly comparing the cuvette holder 5; the described light source 2 and the cuvette holder 5 are arranged in a light-proof Inside the cassette 4 , the measurement and analysis unit 11 is arranged outside the light-proof cassette 4 . The dark box 4 provides a dark environment for the absorbance measurement and eliminates the interference of external light. Preferably, the size of the light-shielding cassette 4 is 180×120×75mm, and the material is black plastic.

[0065] The light-shieldin...

Embodiment 2

[0070] The mensuration of the absorption spectrum of embodiment 2, methyl orange solution

[0071] Select methyl orange standard solution to test the spectral resolving power of the spectrophotometer of the present invention.

[0072] (1) In this embodiment, the size of the dark box is 180×120×75 mm, and its material is black plastic. The mobile phone model used for light source 2 is Huawei Honor VI. The wallpaper pattern used to output monochromatic light is designed with PowerPoint software. The single light-emitting area is rectangular. match.

[0073] According to Dan Bruton's virtual wavelength and RGB value function relationship ( Figure 5 ) to set the RGB value corresponding to the virtual wavelength. In this embodiment, the virtual wavelength range is selected as 400-560 nm, and the wavelength interval is 10 nm. The outer dimensions of the glass cuvette are 34×14×45mm, the absorption path is 30mm, and the solution volume is 9mL. The smartphone model used to captu...

Embodiment 3

[0077] The mensuration of embodiment 3, free chlorine in tap water

[0078] (1) Principle: For the determination of free chlorine in tap water, refer to the national environmental protection standard (HJ 586-2010). The principle is that at a pH of 6.2 to 6.5, free chlorine reacts directly with N,N-diethyl-1,4-phenylenediamine (DPD) to generate a red compound. At a wavelength of 515nm, the spectrophotometric method is used to Measure its absorbance. Since the free chlorine standard solution is unstable and difficult to obtain, this standard uses iodine molecules instead of free chlorine to make a calibration curve. Taking potassium iodate as a benchmark, the following reaction occurs with potassium iodide under acidic conditions:

[0079] IO 3 - +5I - +6H + =3I 2 +3H 2 O, I 2 +I - =[I 3 ] - ,

[0080] The generated iodine molecule or [I 3 ] - A color reaction occurs with DPD, and the ratio of the amount of iodine molecules to chlorine molecules is 1:1.

[0081] ...

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Abstract

The invention relates to a portable multi-channel spectrophotometer based on a smart phone, and a method for measuring absorbance. The spectrophotometer comprises a light-shielding dark box, a light source, a cuvette rack and a measurement and analysis unit, wherein the measurement and analysis unit is the smart phone provided with a camera, and the cuvette rack is located between the light sourceand the measurement and analysis unit, so that the camera of the measurement and analysis unit is right opposite to the cuvette rack; the light source and the cuvette rack are arranged in the light-shielding dark box, and the measurement and analysis unit is arranged outside the light-shielding dark box. The intelligent mobile phone is used as a monochromatic light source generator, a light intensity signal detector and an image gray level and ratio measurement and data platform, has the advantages of being high in accuracy, simple in structure, small in size, light in weight, low in energy consumption, convenient to disassemble and carry, low in cost, high in expansibility and convenient to operate.

Description

technical field [0001] The invention belongs to the field of analysis and detection, and specifically relates to a portable multi-channel spectrophotometer and a method for measuring absorbance. Composed of self-edited image processing software. Background technique [0002] Spectrophotometer is a commonly used analytical instrument, which is widely used in various analysis and detection. The basic principle is to measure the intensity change of a beam of parallel monochromatic light passing through the solution, and the quantitative basis is the Lambert-Beer law, namely: [0003] [0004] In formula (1), A is absorbance, I 0 is the intensity of the incident light, I is the intensity of the transmitted light, ε is the molar absorption coefficient of the light-absorbing substance in the solution at this wavelength (λ), b is the length of the absorption path, and C is the molar concentration of the light-absorbing substance. [0005] When measuring the absorbance of the ...

Claims

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Application Information

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IPC IPC(8): G01N21/31H04M1/725
CPCG01N21/31H04M1/72403Y02D30/70
Inventor 黄薇申其睿
Owner ZAOZHUANG UNIV
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