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Method for preparing human nerve growth factor using transgenic pig salivary gland

A technology of transgenic pigs and growth factors, applied in the directions of nerve growth factor, growth factor/inducing factor, botanical equipment and method, etc., can solve the problem of low production of mouse NGF, solve the problem of homology, and achieve good drug activity , to solve the effect of low output

Inactive Publication Date: 2019-04-16
SOUTH CHINA AGRI UNIV
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Problems solved by technology

Therefore, the method invented by this patent can express human NGF protein with high efficiency and high yield in the obtained transgenic pig saliva, which not only solves the problem of low yield of mouse NGF, but also solves the problem of homology

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  • Method for preparing human nerve growth factor using transgenic pig salivary gland
  • Method for preparing human nerve growth factor using transgenic pig salivary gland
  • Method for preparing human nerve growth factor using transgenic pig salivary gland

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[0030] The present invention will be described in further detail below in conjunction with the accompanying drawings.

[0031] The gene sequence of SEQ ID NO: 2 involved in the present invention is derived from GenBank accession number: NM_002506.2; the gene sequence of SEQ ID NO: 3 is derived from GenBank accession number: NM_002506.2; the gene sequence of SEQ ID NO: 4 is derived from GenBank accession number: X01697.1; SEQ ID NO: 5 gene sequence is derived from Golovan SP et al, Transgenic mice expressing bacterial phytase as a model for phosphorus pollution control, Nature Publishing Group, 2001 article report; SEQ ID NO: 6, SEQ ID NO: 7. The bGH polyA sequence, CMV promoter sequence, Neo-2A-EGFP fusion gene sequence, and bGH polyA sequence are derived from zhenfang Wu et al, Co-expression of two fibrolytic enzyme genes in CHOcells and transgenic mice, Transgenic Research, 2013 article report.

[0032] 1. Construct a transposon vector containing human NGF gene.

[0033]A 1...

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Abstract

The invention discloses a method for preparing a human nerve growth factor using a transgenic pig salivary gland. A transgenic pig which can specifically express human NGF in a parotid gland is prepared through a transgenic method, secreted saliva of the transgenic pig is collected to separate and purify the human NGF to be used for the treatment of human diseases, and the human NGF has a significant effect particularly on the treatment of human neurological diseases. Currently developed and clinically used NGF medicines on the market are murine NGF, murine saliva amount is less, endogenous secreting amount is less, the separation purifying amount is less, so that the marketing price is high; although the homology of murine NGF and human NGF is high, there still exists the problem of immunogenicity in the murine NGF, which might cause untoward reactions of allergy and the like. The method has the advantages that efficient and high-yield human NGF proteins in the transgenic pig are obtained, the problem of low murine NGF yield is solved, and the homology problem is solved.

Description

technical field [0001] The invention relates to an animal obtained by a transgenic method and a preparation method thereof, in particular to a transgenic human nerve growth factor gene pig and a preparation method thereof. Background technique [0002] Nerve growth factor (Nerve Growth Factor, referred to as NGF) is a kind of nerve cell growth regulator with neuron nutrition and neurite growth, and it is the first member of the neurotrophin family. It plays an important regulatory role in various physiological processes. It was first discovered by Levi-Montalcini in 1954, and then Cohen also studied it and clarified its physiological function. Cohen and Levi-Montalcini won the 1986 Nobel Prize in Physiology or Medicine for this. NGF not only plays an important role in the proliferation and differentiation of nerve cells, but also plays an important auxiliary role in the growth and activity of non-nerve cells, such as immune cells, fibroblasts, pancreatic cells, and cardiom...

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Application Information

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IPC IPC(8): C12N15/85C12N15/877A01K67/027C07K14/48
CPCA01K67/0278A01K2207/15A01K2217/072A01K2227/108A01K2267/01C07K14/48C12N15/8509C12N15/8778C12N2800/107
Inventor 吴珍芳曾芳廖莎李紫聪朱庆春
Owner SOUTH CHINA AGRI UNIV
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