Preparation method of detection reagent for porcine circovirus type 2

Abstract

The invention relates to the technical field of animal disease detection, and discloses a preparation method of a detection reagent for porcine circovirus type 2. The preparation method specifically comprises the following steps of S1, expression of recombinant ORF2 protein, wherein pET-28a-ORF2 is converted into escherichia coli BL21 competent cells, the positive escherichia coli BL21 competent cells are selected for clone and inoculated into an LB culture medium containing kanamycin, overnight culture is performed, 50 microliters of a bacteria solution is taken the next day to be inoculatedinto 5 milliliters of the LB culture medium containing the kanamycin, culture is performed for 2 h until the OD600 value is 0.6-0.8, IPTG with the final concentration of 1 millimeter is added, sampling is carried out after induction is performed for 8 h, and a strain pET-28a-BL21 is induced as a negative control, and the protein expression situation is identified by SDS-PAGE gel electrophoresis; S2, soluble analysis of the recombinant ORF2 protein, wherein 2 microliters of a recombined strain pET-28a-ORF2-BL21 is added into 2 milliliters of the LB culture medium containing the kanamycin. The preparation method of the detection reagent for the porcine circovirus type 2 can more effectively improve the production efficiency, and can also improve the accuracy of the detection reagent for detecting the circovirus type 2.

Description

technical field [0001] The invention relates to the technical field of veterinary disease detection, in particular to a method for preparing a porcine circovirus type 2 detection reagent. Background technique [0002] Porcine circovirus type 2 belongs to the genus Circovirus in the family Circoviridae in taxonomy, and is one of the smallest known animal viruses. It can also survive in a high temperature environment for a period of time, it will not be inactivated by the action of chloroform, and has no hemagglutination activity. Porcine circovirus type 2 is a pathogenic virus. Pigs are highly susceptible to porcine circovirus type 2. Infected pigs can excrete the virus from nasal fluid, feces and other wastes, and infect people of different ages through oral and respiratory routes. pig. Therefore, in order to prevent large-scale infection of porcine circovirus type 2, it is necessary to use porcine circovirus type 2 detection reagents to detect pigs. [0003] The existing...

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies in the prior art, the invention provides a method for preparing a porcine circovirus type 2 detection reagent, which has the advantages of being able to improve the production efficiency of the circovirus type 2 detection reagent, and solves the current problem of circovirus type 2 detection reagents on the market. The problem of low production efficiency of virus type 2 detection reagents