Gene RKcrtYB and applications thereof

Abstract

The invention discloses a gene RKcrtYB. The gene is isolated from rhodosporidium kratochvilovae YM25235. The nucleotide sequence of the gene is as shown in SEQ ID NO:1, and the amino acid sequence encoded by the gene is as shown as EDQ ID NO:2. In the present invention, the gene is linked into a vector and transferred into the YM25235, and the translated protein is involved in the synthesis of beta-carotenoids. The gene can enable the rhodospora erythraea YM25235 to improve the synthesis of the beta-carotenoids, and lays a foundation for the large-scale commercial production of the beta-carotenoids.

Description

technical field [0001] The invention belongs to the field of microbial genetic engineering in the field of biotechnology, in particular to Rhodosporidium kratochvilovae ) cloned from YM25235 RKcrtYB Then connect the gene to the carrier and transfer it into YM25235, and use it to regulate the synthesis of key enzymes in the synthesis of carotenoids in YM25235, and then can produce a large amount of β-carotenoids, which is high-yield and high-quality β-carotenoids Provide a reference for the industrial production of element. Background technique [0002] Carotenoids are a class of polyene compounds that widely exist in nature. They are red, orange or yellow lipophilic isoprenoid pigments produced by plants and some photosynthetic microorganisms. The existence of the isoprene palace distructure has strong light absorption, so there is generally an absorption peak between the wavelength of 430-570nm. At present, more than 700 kinds of carotenoids have been found in nature. ...

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Problems solved by technology

[0004] Carotenoids are extremely unstable under the conditions of oxygen, high temperature, light, and strong acid, and are easy to deteriorate, degrade, or isomerize. The usual storage method is to store in isolation from air, low temperature, and dark, because it is relatively stable in an alkaline environment , so the industry usually uses alkaline saponification treatment to extract carotenoids. Although the cost of the carotenoids extracted in this way is low, the biological activity is low, and the output and quality of the carotenoids extracted in this way are far from meeting people's needs; Therefore, it is a research hotspot in recent years to screen microorganisms with excellent traits from nature for carotenoid biosynthesis instead of chemical synthesis.

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