Composition of collagen peptide and elastin peptide, and preparation method and application of composition
A technology of elastin peptide and collagen peptide, which is applied in the direction of chemical instruments and methods, biochemical equipment and methods, applications, etc., can solve the problems of high cost and increase consumer cost, and achieve the reduction of hydroxyproline content, inhibition of The effect of skin inflammatory factors, increased content
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Embodiment 1
[0037] Embodiment 1: preparation composition of the present invention
[0038]Cut the cod skin into pieces, dry it in an oven at 80°C for 12 hours, then pulverize it into powder with a pulverizer, and store it in a cool and dry place for later use. Weigh cod skin powder, add 20-30 times the amount of water, heat the solution to 60°C±5°C, add 1% pepsin to the solution, enzymatically hydrolyze for 4-8 hours, heat up to 80±5°C, and keep warm for 0.5-1 hours, inactivate the enzyme, then pass the enzymolysis solution through a 1 μm filter membrane, maintain the temperature at 55±5°C, and take the filtrate. Collagen peptides were obtained after the filtrate was spray-dried. The molecular weight of collagen determined by high performance size exclusion chromatography is 2000Da~6000Da;
[0039] Take bovine ligament and wash it, add 0.5% NaOH solution, soak at 40-50°C for 1-2 hours, remove surface fat and impurities, wash to the center, drain and mince to obtain crude elastin extract...
Embodiment 2
[0041] Embodiment 2: animal experiments
[0042] 1. Experimental materials
[0043] D-galactose: purchased from Sigma-Aldrich; Mouse Hyaluronic acid (HA) ELISA kit, Mouse hydroxyproline (Hyp) ELISA kit, Mouse Collagen Type I (ColI) ELISA kit, MMP3 ELISA kit, Mouse Elastin ELISA kit, IL-1α ELISA kit The kit and IL-4 kit were purchased from Wuhan Huamei Bioengineering Co., Ltd.; GSH-PX was purchased from Nanjing Jiancheng Bioengineering Institute.
[0044] 2. Experimental equipment
[0045] Electronic balance: Mettler Toledo, model: PL303; skin moisture meter: Huntkey portable elastic moisture oily skin tester (HUNTKEY JAPAN Co., Ltd.), model: HKJ-SK03P; UV sterilization lamp: Philips black light tube TL-D ultraviolet sterilization Lamp (Philips Lighting Company), model: TL-D / BLB; centrifuge: Kylin-BellLabInstruments, model: LX-200; microplate reader: American BIO-TEK, model: ELX-800; homogenizer: SONICSMATERIALS INC, Model: VCX130PB; EP tube, syringe, etc.
[0046] 3. Exper...
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