Copper nano-cluster sweat latent fingerprint developing reagent preparation method and sweat latent fingerprint developing method
A technology of copper nano-cluster and developing agent, which is applied in the field of sweat latent fingerprint display and preparation of copper nano-cluster sweat latent fingerprint display reagent, can solve the problems of background color influence, high price, difficulty and the like, and achieves eliminating the interference of background color and making The effect of simple process and easy surface modification
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Embodiment 1
[0038] The preparation method of the copper nano-cluster sweat latent fingerprint display reagent comprises the following steps:
[0039] S1, branched polyethyleneimine, ascorbic acid, and copper sulfate are used as raw materials to prepare a visualization reagent; specifically, the following steps are included:
[0040] S101, dissolve BPEI in ultrapure water to prepare a 0.01g / ml solution, and ultrasonicate for 5-10min; dissolve AA in ultrapure water to prepare a 0.1M / ml solution; 4 Soluble in ultrapure water, and make a 0.1g / ml solution;
[0041] S102, take 0.1ml of BPEI solution, drop it in 10ml of ultrapure water, slowly add CuSO dropwise under stirring 4 Solution 1ml (0.625mM), after 3-5min, add 1mlAA (0.1M) dropwise;
[0042] S103, respectively reacting at 30°C, 60°C, 90°C, 120°C, and 150°C for 6 to 8 hours to obtain copper nanocluster solutions at various temperatures;
[0043] S104, mixing and stirring the solution, and ultrasonicating for 10-15 minutes.
Embodiment 2
[0045] The preparation method of the copper nano-cluster sweat latent fingerprint display reagent comprises the following steps:
[0046] S101, dissolve BPEI in ultrapure water to prepare a 0.01g / ml solution, and ultrasonicate for 6-8 minutes; dissolve AA in ultrapure water to prepare a 0.1M / ml solution; 4 Soluble in ultrapure water, and make a 0.1g / ml solution;
[0047] S102, take 0.1ml of BPEI solution, drop it in 10ml of ultrapure water, slowly add CuSO dropwise under stirring 4 Solution 1ml (0.625mM), after 3-5min, add 1mlAA (0.1M) dropwise;
[0048] S103, respectively reacting at 30°C, 60°C, 90°C, 120°C, and 150°C for 6 to 8 hours to obtain copper nanocluster solutions at various temperatures;
[0049] S104, mixing and stirring the solution, and ultrasonicating for 12-14 minutes.
Embodiment 3
[0051] A method for showing latent sweat fingerprints, comprising the steps of:
[0052] S201, reagent preparation, preparation of copper nano-cluster sweat latent fingerprint display reagent;
[0053] S202, press the fingerprint, the volunteer presses the fingerprint on the surface of the object to make a sample of the fingerprint;
[0054] S203, displaying fingerprints by dripping, respectively displaying the latent sweat fingerprints on different objects with the visualization reagent in step S201;
[0055] S204, drying the dripping latent fingerprint in a vacuum oven at 30-50°C;
[0056] S205, using multi-band light sources, colored filters, and digital SLR cameras to independently build a display observation system to observe fluorescent sweat latent fingerprints, and take pictures and fix them;
[0057] S206, in the darkroom, irradiate the latent sweat fingerprints displayed by the reagent drops prepared by the method with a multi-band light source, the fluorescence ge...
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