DNA quantitative analysis method based on microscope images of cells
A quantitative analysis and microscopy technology, applied in the field of image processing, can solve the problems of affecting accuracy, time-consuming, missing diploid cells, etc., and achieve the effect of improving accuracy and stabilizing results
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[0018] The present invention will be described in detail below with reference to the drawings in the embodiments of the present invention.
[0019] Such as figure 1 As shown, a DNA quantitative analysis method based on a cell microscope image disclosed in the present invention comprises the following steps:
[0020] Step 1: Position the cell-laden slide in the detection center of the microscope stage so that the cell-enriched area is under the observation line of sight of the microscope;
[0021] Step 2: Focus on the cell plane and take a picture, save the picture after taking the picture, and use the information file directly obtained from the CCD or CMOS as the analysis picture;
[0022] Further, the information files obtained directly from the CCD or CMOS are RAW files.
[0023] Step 3: Analyze the image after taking the photo: collect normal diploid, abnormal ploid, lymphocyte, and other cell images, and calculate the integral optical density value of the nucleus after i...
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