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Poria cocos oligosaccharide functionalization nanometer selenium and preparation and application thereof

A technology for the functionalization of oligosaccharides, which is applied in the field of functionalized nano-selenium of oligosaccharides and its preparation, can solve the problems of unsatisfactory treatment effect and serious side effects, and achieve the goal of reducing economic burden, high bioavailability, and low price. Effect

Inactive Publication Date: 2019-03-29
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the effect of clinical drug treatment is not satisfactory, and the side effects of long-term application are serious

Method used

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  • Poria cocos oligosaccharide functionalization nanometer selenium and preparation and application thereof
  • Poria cocos oligosaccharide functionalization nanometer selenium and preparation and application thereof
  • Poria cocos oligosaccharide functionalization nanometer selenium and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: Preparation of Poria cocos oligosaccharide functionalized nano-selenium

[0041] (1) Under normal temperature and pressure, add β-mannanase to an aqueous solution of pachyrhin (PPS) (purchased from Shanghai Yuanye Biotechnology Co., Ltd., product number: S25585) with a mass concentration of 10.0 mg / mL. The concentration of β-mannanase was 5 units (U) / mL, stirred and reacted in a water bath at 37°C for 2 hours, and the oligosaccharide fraction was collected by gel filtration chromatography, which was pachyoligosaccharide (POS).

[0042] In step (1), the sugar composition and standard sugar profile of pachyrhiza before and after being hydrolyzed by β-mannanase are as follows: figure 1 shown, from figure 1 It can be seen from the figure that Poria cocos polysaccharide (purchased from Shanghai Yuanye Biotechnology Co., Ltd., product number: S25585) mainly contains fucose, galactose, glucose and mannose, and was separated and collected after being hydrolyzed by β...

Embodiment 2

[0048] Example 2: Poria cocos oligosaccharide functionalized nano-selenium antagonizes enteritis

[0049] Group experiments on mice were carried out in the National Licensed Animal Laboratory of Jinan University School of Medicine. Fifty male C57BL / 6 mice were randomly divided into 5 groups, 10 in each group, respectively (1) blank control group, (2) enteritis model group, (3)-(5) experimental dose group. After normal feeding for 1 week in separate cages, mice in groups (2)-(5) were induced with drinking water containing 4% DSS to continue the acute colitis model for 5 days, while mice in groups (3)-(5) Rats were fed with different doses of POS-SeNPs (0.08, 0.4 and 1.0 mg / kg body weight respectively according to the amount of selenium administered). The body weight, diarrhea, hematochezia and other symptoms of mice in each group were recorded in detail every day, and fecal occult blood test was detected. At the end of the experiment, the mice were killed, and the blood, colo...

Embodiment 3

[0053] Example 3: Application Prediction of Poria Cocos Oligosaccharide Functionalized Nano Selenium

[0054] Poria oligosaccharide functionalized nano-selenium (POS-SeNPs) prepared in Example 1 of the present invention is converted into functional food or health product preparations for the auxiliary treatment of enteritis and other inflammatory diseases, resulting in large-scale social and economic benefits. Taking my country as an example, there are currently about 6 million patients with enteritis. It is estimated that each person uses this product at 20 yuan per day for a 3-month course of treatment. amounted to more than 100 million yuan. The application transformation of the patent of this invention will promote the raw material industry of food and medicinal polysaccharides and selenium powder, and produce tablets, capsules and oral liquid series products to assist in the treatment of other inflammatory diseases, forming its own patented technology pool and transformati...

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Abstract

The invention belongs to the biological technical field of biopharmaceutical Chinese patent medicines and functional foods, and discloses a preparation method and an application of poria cocos oligosaccharide functionalization nanometer selenium (POS-SeNPs). Poria cocos oligosaccharide is water-soluble mannose-enriched glucose-mannose oligosaccharide obtained through hydrolyzing poria cocos polysaccharide by beta-mannase. The poria cocos oligosaccharide is used as an adjusting and controlling agent, in a vitamin C reduction sodium selenite system, poria cocos oligosaccharide functionalizationmodified nanometer selenium POS-SeNPs is prepared, and nanometer dimension uniformity, liquid phase storage stability and oligosaccharide modification high efficiency are realized. The poria cocos oligosaccharide functionalization nanometer selenium products are mainly suitable for auxiliary treatment of inflammatory bowel diseases, can also be used for auxiliary prevention and treatment of hyperlipemia, atherosclerosis, diabetes, intestine tumors and other inflammation relevant diseases. The poria cocos oligosaccharide functionalization nanometer selenium products are simple and convenient touse, green and safe, and low in cost, pain of patients can be relieved, and economic burden can be greatly reduced.

Description

technical field [0001] The invention belongs to the technical field of biomedical Chinese patent medicine and functional food, and in particular relates to a functionalized nano-selenium of poria cocos oligosaccharide and its preparation method and application. Background technique [0002] The traditional Chinese medicine Poriacocos Wolf is the dried sclerotia of the fungus Poriacocos in the family Polyporaceae. Poria cocos is a famous and precious Chinese medicinal material in China. It is flat in nature, sweet and light in taste, and enters the heart, lung, spleen, and kidney channels. . The main active ingredient of Poria cocos is Poria cocospolysaccharides (PPS), which has good anti-tumor, anti-infection, anti-aging and regulating immunity and metabolism. The monosaccharide composition, sugar chain structure, and biochemical characteristics of pachyrhin polysaccharide PPS are significantly different from those of polysaccharides derived from other species. In order to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/04A61K47/61A61P1/00A61P3/06A61P9/10A61P3/10A61P35/00A23L33/125A23L33/15A23L33/165A61K31/736
CPCA23L33/125A23L33/15A23L33/165A23V2002/00A61K31/736A61K33/04A61K47/61A61P1/00A61P3/06A61P3/10A61P9/10A61P35/00A23V2200/32A23V2200/308A23V2200/326A23V2200/3262A23V2200/328A23V2250/1626A23V2250/708A61K2300/00
Inventor 黄峙凌钦婕朱承慧杨晖武彦君
Owner JINAN UNIVERSITY
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