Method for increasing sterilizing efficiency of aminoglycoside antibiotics by taking ethyl alcohol as sensitizer

An aminoglycoside and sterilization efficiency technology, applied in the field of improving the sterilization efficiency of aminoglycoside antibiotics, can solve problems such as ototoxicity, bacterial resistance to antibiotics, nephrotoxicity, etc., to reduce side effects, reduce administration time, and improve sterilization efficiency. Effect

Active Publication Date: 2019-03-29
FUJIAN NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This class of antibiotics has many limitations in clinical use, mainly due to the growing phenomenon of bacterial resistance and nephrotoxicity and ototoxicity of this class of antibiotics

Method used

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  • Method for increasing sterilizing efficiency of aminoglycoside antibiotics by taking ethyl alcohol as sensitizer
  • Method for increasing sterilizing efficiency of aminoglycoside antibiotics by taking ethyl alcohol as sensitizer
  • Method for increasing sterilizing efficiency of aminoglycoside antibiotics by taking ethyl alcohol as sensitizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Effects of Common Alcohols on Escherichia coli Killed by Tobramycin

[0023] 1. Activate Escherichia coli (E. coli k-12BW25113 (Escherichia coli k-12BW25113): absorb 1 μl of 20% glycerol bacteria solution of Escherichia coli BW25113 stored in a -80°C refrigerator, add it to 1ml LB liquid medium, and 37°C shaker (250rpm) cultivated to the plateau stage, the obtained bacterial solution was diluted 1000 times and inoculated in 20ml LB liquid medium, and 37°C shaker (250rpm) was cultivated overnight (20 hours) to obtain Escherichia coli culture solution.

[0024] 2. Take 18ml of Escherichia coli culture solution obtained in step 1, divide it into 18 sterile shaking tubes, and divide it into 2 groups randomly, namely the no antibiotic group and the tobramycin group, with 1ml of bacterial solution in each tube.

[0025] In the 9 shaking tubes of the antibiotic-free group, alcohol was not added to 1 shaking tube, and methanol, ethanol, n-propanol, isopropanol, n-butanol, isobu...

Embodiment 2

[0036]Ethanol enhances the efficiency of aminoglycoside antibiotics against Escherichia coli

[0037] 1. Activate Escherichia coli (E. coli k-12BW25113 (Escherichia coli k-12BW25113): absorb 1 μl of 20% glycerol bacteria solution of Escherichia coli BW25113 stored in a -80°C refrigerator, add it to 1ml LB liquid medium, and 37°C shaker (250rpm) cultivated to the plateau stage, the obtained bacterial solution was diluted 1000 times and inoculated in 20ml LB liquid medium, and 37°C shaker (250rpm) was cultivated overnight (20 hours) to obtain Escherichia coli culture solution.

[0038] 2. Take 10 mL of the E. coli culture solution obtained in step 1, divide it into 10 sterile shaking tubes, and divide it into 5 groups randomly, namely no antibiotic group, tobramycin group, gentamicin group, streptomycin group, and streptomycin group. In the plain group and the kanamycin group, each tube was filled with 1mL bacterial solution.

[0039] Add tobramycin to the two shaking tubes in ...

Embodiment 3

[0053] Ethanol enhances the efficiency of aminoglycoside antibiotics against Pseudomonas aeruginosa

[0054] 1. Activation of Pseudomonas aeruginosa (P.aeruginosa) PAO1: absorb 1 μl of 20% glycerol bacterial solution of Pseudomonas aeruginosa (P.aeruginosa) PAO1 stored in a -80°C refrigerator, add to 1ml LB liquid medium in 37°C shaker (250rpm) to the plateau stage, the obtained bacterial solution was diluted 1000 times and inoculated in 20ml LB liquid medium, and cultivated overnight (24 hours) in a 37°C shaker (250rpm) to obtain pseudo aeruginosa Single cell culture medium.

[0055] 2. Take 6 mL of the Pseudomonas aeruginosa culture solution obtained in step 1, divide it into 6 sterile shaking tubes, and divide it into 3 groups randomly, namely no antibiotic group, tobramycin group, and gentamicin group , each tube was filled with 1mL bacterial solution.

[0056] Add tobramycin to the two shaking tubes in the tobramycin group, the concentration of tobramycin in the bacteri...

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Abstract

The invention discloses a method for increasing sterilizing efficiency of aminoglycoside antibiotics by taking ethyl alcohol as a sensitizer. The method comprises the following steps: adding ethyl alcohol with a final concentration of 20-30 mM into a bacteria solution containing to-be-killed bacteria, thereby obtaining a pre-treated bacteria solution; adding aminoglycoside antibiotics into the pre-treated bacteria solution, thereby sterilizing. According to the method disclosed by the invention, the sterilizing efficiency of aminoglycoside antibiotics on Gram negative bacteria can be greatly promoted; the risk of pathogenic bacteria generating drug resistance can be effectively reduced; dosage and drug administration time can be reduced under the condition of achieving a same therapeutic effect, so that side effects thereof can be reduced.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for improving the bactericidal efficiency of aminoglycoside antibiotics by using ethanol as a sensitizer. Background technique [0002] Antimicrobial resistance is a major public health problem facing the world. In China, due to the abuse of clinical antibiotics and the abuse of antibiotics in the breeding industry, the problem of bacterial resistance is more urgent. Improving the bactericidal efficiency of existing antibiotics and killing pathogenic bacteria quickly and efficiently is an important means to reduce the risk of bacterial resistance. [0003] Escherichia coli is an important bacterium that parasitizes the human intestinal tract and can cause disease under special circumstances, such as blood infection. At the same time, Escherichia coli, as a standard strain commonly used in biological research, is widely used in the study of the mechanism of bacterial drug r...

Claims

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Application Information

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IPC IPC(8): A01N31/02A01N43/16A01P1/00
CPCA01N31/02A01N43/16
Inventor 付新苗李中燕张祖勤高媛媛
Owner FUJIAN NORMAL UNIV
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