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Method for measuring four kinds of phenolic substances in myrtle fruit simultaneously

A technology of myrtle fruit and gallic acid is applied in the field of chemical composition detection of Chinese medicinal materials to achieve the effects of good reproducibility, less reagent consumption and high sensitivity

Inactive Publication Date: 2019-03-26
GUANGXI VETERINARY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is, based on ultra-high performance liquid chromatography (UPLC) technology, to establish a method that is simpler, faster, less reagent consumption, high sensitivity, and can simultaneously determine gallic acid and ellagic acid in myrtle fruit. , resveratrol, picetanol content method

Method used

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  • Method for measuring four kinds of phenolic substances in myrtle fruit simultaneously
  • Method for measuring four kinds of phenolic substances in myrtle fruit simultaneously
  • Method for measuring four kinds of phenolic substances in myrtle fruit simultaneously

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Configuration of standard solution: Accurately weigh the reference substances of gallic acid, ellagic acid, resveratrol, and picatanol in a brown volumetric flask, add methanol to dissolve and dilute to the mark, shake well, and prepare respectively Reference solution of gallic acid 123.40 μg / mL, ellagic acid 370.05 μg / mL, piceatanol 242.26 μg / mL, resveratrol 225.50 μg / mL; then dilute the above standard solution with methanol in sequence to prepare different concentrations standard working solution.

[0029] (2) Sample pretreatment: Guangdong Meizhou myrtle medicinal material was powdered, passed through a 60-mesh sieve, and 2.0g of fine powder was accurately weighed. As for a 50ml conical flask with a stopper, 30mL of 95% ethanol was added accurately and ultrasonically (350W, 53kHz, 30°C) for extraction for 30 minutes, let cool, filter, store in a brown volumetric flask, and pass through a 0.2 μm filter membrane before content testing.

[0030] (3) Chromatographic...

Embodiment 2

[0043] (1) The configuration of the standard solution is the same as in Example 1.

[0044] (2) Sample pretreatment: Guangxi Pingle myrtle medicinal material is powdered, passed through a 60-mesh sieve, and 3.0 g of fine powder is accurately weighed. As for a 100 ml conical flask with a stopper, 50 mL of 95% ethanol is precisely added and ultrasonically (350 W, 53 kHz , 30°C) for extraction for 40 minutes, let cool, filter, store in a brown volumetric flask, and pass through a 0.2 μm filter membrane before content testing.

[0045] (3) The chromatographic conditions are the same as in Example 1.

[0046] (4) The linear range, precision and detection limit of the method are the same as in Example 1.

[0047] (5) Stability test: Accurately weigh the sample powder of Guangxi Pingle myrtle fruit, prepare the test product according to the sample pretreatment method in step (2), and carry out the test at 0, 4, 8, 12, 24, and 48 hours after preparation respectively. to measure the ...

Embodiment 3

[0056] (1) The configuration of the standard solution is the same as in Example 1.

[0057] (2) Sample pretreatment: Guangxi Wuzhou myrtle medicinal material is powdered, passed through a 60-mesh sieve, and 2.0g of fine powder is accurately weighed. As for a 100ml conical flask with a stopper, 30mL of 95% ethanol is precisely added to it for ultrasonication (350W, 53kHz, 30°C) for extraction for 40 minutes, let cool, filter, store in a brown volumetric flask, and pass through a 0.2 μm filter membrane before content testing.

[0058] (3) The chromatographic conditions are the same as in Example 1.

[0059] (4) The linear range, precision and detection limit of the method are the same as in Example 1.

[0060] (5) Stability test: Accurately weigh the myrtle fruit sample powder in Wuzhou, Guangxi, prepare the test sample according to the sample pretreatment method in step (2), and inject samples at 0, 4, 8, 12, 24, and 48 hours after preparation respectively , to measure the pe...

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Abstract

The invention provides a ultra-high performance liquid chromatography for measuring four kinds of phenolic substances including gallic acid, ellagic acid, piceatannol and resveratrol in a myrtle fruitsimultaneously. A pre-treated to-be-measured sample is detected by a ultra-high performance liquid chromatographic instrument according to the following conditions: gradient elution, the mobile phaseis acetonitrile-0.2% phosphoric acid; elution condition: 6% acetonitrile for 0-2 minutes, 6%-20% acetonitrile for 2-4 minutes, 20%-60% acetonitrile for 4-10 minutes, and 6% acetonitrile for 10-12 minutes; the detection wavelength is 215-380 nm; the flow speed is 0.1-0.6 ml / min; the column temperature is 25-50 DEG C; the sample injection volume is 0.5-15 mu L; a diode array detector; Waters CORTECS UPLC T3 chromatographic column (2.1 mm*100 mm, 1.6 microns). The method is simple, convenient and rapid, a reagent is consumed less, the stability is good, and the sensitiveness is high.

Description

technical field [0001] The invention relates to the field of detection of chemical components of traditional Chinese medicinal materials, in particular to a method for simultaneously detecting four phenolic components in myrtle fruit, gallic acid, ellagic acid, piceatanol and resveratrol, by using ultra-high performance liquid chromatography method. Background technique [0002] Myrtle Rhodomyrtus tomentosa (Ait.) Hassk. is an evergreen multi-flowered shrub of Myrtaceae Myrtaceae, also known as Renzi, Shanrenzi, etc. It is an excellent wild plant resource, mainly distributed in Southeast Asian countries, It is especially abundant in the southern regions of China and Japan, and its fruits are rich in various active ingredients such as flavonoids, polyphenols and polysaccharides. It is known that phenolic substances have pharmacological effects such as anti-oxidation, anti-tumor, and anti-inflammation. The study of polyphenols in myrtle fruit has good academic value and devel...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 银慧慧刘伟赵武孟菲姜源明覃振华孙建华
Owner GUANGXI VETERINARY RES INST
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