Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cellulase-producing bacillus licheniformis, microbial fermentation preparation thereof and application of cellulase-producing bacillus licheniformis

A technology of Bacillus licheniformis and microbial fermentation, applied in the direction of microorganisms, microorganisms, and methods based on microorganisms, to achieve the effects of facilitating digestion and absorption, short fermentation time, and high number of viable bacteria

Inactive Publication Date: 2019-03-26
JILIN COFCO BIOCHEM +2
View PDF7 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no Bacillus licheniformis with higher cellulase activity for cellulose in processed corn products in this field.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cellulase-producing bacillus licheniformis, microbial fermentation preparation thereof and application of cellulase-producing bacillus licheniformis
  • Cellulase-producing bacillus licheniformis, microbial fermentation preparation thereof and application of cellulase-producing bacillus licheniformis
  • Cellulase-producing bacillus licheniformis, microbial fermentation preparation thereof and application of cellulase-producing bacillus licheniformis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1: Isolation and screening of Bacillus licheniformis

[0059] Take 10g of Shanxi Fenjiu Daqu sample in 90mL of sterile saline, place on a shaker, shake at 30°C and 200rpm for 30min, and then dilute 10 times to 10 -8 . Take 1mL10 -8 Add 20mL 80°C solid medium of Bacillus licheniformis in a melted state to a 6cm plate, mix well and cool to room temperature, and after solidification, place it upside down in a 30°C constant temperature incubator for 24 hours. After 24 hours, it can be observed on the plate that multiple colonies are formed. The colonies are round colonies with irregular edges and wrinkles on the surface, and are positive by Gram staining; The morphology is long rod-like, single, paired or chain-like; this feature is the same as that of Bacillus licheniformis.

[0060] Pick a single colony (concentration is 10 9 CFU / mL) were inoculated on a CMC screening medium plate, and cultured upside down at 30°C for 24 hours. After 24 hours, 5 mL of 1w / v% C...

Embodiment 2

[0062] Example 2: Identification of Bacillus licheniformis 50

[0063] 2.1 Identification of colony morphology

[0064] The strain isolated in Example 1 was observed by biological characteristics, and further morphological identification (comprising: observation of the shape and edge of the colony, Gram staining, etc.) was carried out. The above-mentioned identification standards refer to "Berger's Bacteria Identification Handbook", published in Science Society, 1984 and "Common Bacterial System Identification Manual", Science Press, 2001. After the isolated bacterial strain was cultivated at 30°C for 24 hours on the solid medium of Bacillus licheniformis, the colony form was a round colony with irregular edges and wrinkles on the surface, which was positive by Gram staining; Observed below, the thalline shape grows rod-shaped, single, paired or chain-like arrangement; this feature is the same as the morphological feature of Bacillus licheniformis.

[0065] 2.2 Taxonomic...

Embodiment 3

[0069] The preparation of embodiment 3 microbial fermentation agent

[0070] Bacillus licheniformis 50, Lactobacillus plantarum (isolated from commercially available fermented milk) and Candida utilis (isolated from commercially available fermented feed) cultured bacterial liquid were obtained respectively by the following steps:

[0071] (1) Bacillus licheniformis: The Bacillus licheniformis 50 obtained in Example 1 was inoculated into the Bacillus licheniformis culture medium at an inoculation amount of 1 v / v%, placed on a constant temperature shaker, and cultivated overnight at 30° C. and 200 rpm.

[0072](2) Lactobacillus plantarum: Lactobacillus plantarum was inoculated into MRS medium at an inoculation amount of 1v / v%, placed on a constant temperature shaker, and cultured overnight at 30°C and 200rpm.

[0073] (3) Candida utilis: Candida utilis was inoculated into YPD medium at an inoculum size of 1v / v%, placed on a constant temperature shaker, and cultured overnight at ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Vitalityaaaaaaaaaa
Enzyme activityaaaaaaaaaa
Login to View More

Abstract

The invention relates to cellulase-producing bacillus licheniformis 50, a microbial fermentation preparation thereof and application of of the cellulase-producing bacillus licheniformis 50. The cellulase-producing bacillus licheniformis is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.15012. The bacillus licheniformis 50 disclosed by the invention can produce cellulase with high yield and improve the degradation capacity on cellulose in corn processing by-products, and can be applied to feed fermentation with the cornprocessing by-products as raw materials to decrease the fiber content in fermented feed, so as to improve the quality of the feed and facilitate the efficient use of the feed by livestock and poultry.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular, the invention relates to a cellulase-producing Bacillus licheniformis, a microbial fermentation preparation containing the strain and its application in the fermentation of feed containing corn processing by-products. Background technique [0002] Corn is the main crop product in my country, with an annual output of about 200 million tons. Among them, for every ton of corn directly processed, 0.09 tons of corn steep liquor, 0.07 tons of germ meal, 0.056 tons of corn gluten meal and 0.13 tons of corn bran can be produced; at the same time, corn is used as raw material for fermentation to produce many fermentation products, such as fuel ethanol , citric acid, amino acids, etc.; can also produce a large number of by-products, such as DDGS, citric acid residues, etc. Corn processing products such as corn bran, DDGS, citric acid grains, etc. are usually directly added to feed for ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C12N1/16A23K10/12A23K10/37A23K10/30A23K10/38C12R1/10
CPCA23K10/12A23K10/30A23K10/37A23K10/38C12N1/16C12N1/20C12R2001/10C12N1/205A23V2400/517A23V2400/169Y02P60/87
Inventor 佟毅张子剑杨鑫郑晓卫陈博卢宗梅陈影安泰
Owner JILIN COFCO BIOCHEM
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com