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Thermosensitive hydrogel loaded with copper metal organic skeleton nanoparticles and preparation method of thermosensitive hydrogel

A temperature-sensitive hydrogel and nanoparticle technology, used in pharmaceutical formulations, pharmaceutical sciences, drug delivery, etc., can solve problems such as unfavorable wound healing, nano-silver burst release, and poor sustained-release performance, and achieve the goal of promoting wound healing and promoting healing. Effect

Inactive Publication Date: 2019-03-26
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Chinese invention patent application CN201610219873.0 discloses a preparation method of a medical temperature-sensitive hydrogel containing nano-silver and its application in the field of wound healing. It is composed of nano-silver and chitosan, but due to the strong cytotoxicity of nano-silver, In the process of sterilization, normal cells will be damaged, and the hydrogel composed of chitosan has poor slow-release performance of drugs, which is easy to cause the explosive release of nano-silver, which is not conducive to wound healing

Method used

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  • Thermosensitive hydrogel loaded with copper metal organic skeleton nanoparticles and preparation method of thermosensitive hydrogel
  • Thermosensitive hydrogel loaded with copper metal organic skeleton nanoparticles and preparation method of thermosensitive hydrogel
  • Thermosensitive hydrogel loaded with copper metal organic skeleton nanoparticles and preparation method of thermosensitive hydrogel

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Embodiment 1

[0036] The preparation process of the temperature-sensitive hydrogel loaded with copper metal organic framework nanoparticles in this embodiment is as follows: figure 1 As shown, the acylation reaction of amino groups on gelatin and methacrylic anhydride is used to synthesize branched methacrylated gelatin (GelMA) containing carbon-carbon double bonds, and then the carbon-carbon on the branched gelatin is esterified by methacrylic acid. The double bond and N-isopropylacrylamide (NIPAM) were subjected to free radical copolymerization to prepare methacrylated gelatin-g-polyN-isopropylacrylamide (GelMA-g-PNIPAM) temperature-sensitive hydrogel.

[0037] Specifically, the preparation method of the temperature-sensitive hydrogel loaded with copper metal organic framework nanoparticles in this embodiment includes the following steps:

[0038] 1) In a 100 ml flask equipped with a magnetic stir bar, dissolve 6 g of pork skin gelatin in 60 ml of phosphate buffered saline (PBS, pH=7.4), and st...

Embodiment 2

[0046] The present invention utilizes the acylation reaction between amino groups on gelatin and methacrylic anhydride to synthesize branched methacrylated gelatin (GelMA) containing carbon-carbon double bonds, and then uses methacrylate to esterify the carbon-carbon double Free radical copolymerization of bond and N-isopropylacrylamide (NIPAM) to prepare methacrylated gelatin-g-polyN-isopropylacrylamide (GelMA-g-PNIPAM) temperature-sensitive hydrogel; loaded with copper metal organic The preparation method of the temperature-sensitive hydrogel of framework nanoparticles includes the following steps:

[0047] 1) In a 100ml flask equipped with a magnetic stirrer, dissolve 6g of pork skin gelatin in 60ml of 0.25M carbonate buffered saline (CB, pH=9), and stir to fully dissolve it at 50°C. Drop 0.72ml of methacrylic anhydride (MAA) into the gelatin solution at a rate of 0.1mL / min, and react with uniform stirring at 300rpm. Every 30min, adjust the pH of the solution to 9 with 0.5mol / ...

Embodiment 3

[0053] In this example, the amino groups on gelatin and methacrylic anhydride were acylated to synthesize branched methacrylic acid esterified gelatin (GelMA) containing carbon-carbon double bonds, and then methacrylic acid was used to esterify the carbon-carbon on the branched gelatin. The double bond and N-isopropylacrylamide (NIPAM) were subjected to free radical copolymerization to prepare methacrylated gelatin-g-polyN-isopropylacrylamide (GelMA-g-PNIPAM) temperature-sensitive hydrogel. The preparation method of the temperature-sensitive hydrogel loaded with copper metal organic framework nanoparticles includes the following steps:

[0054] 1) In a 100 ml flask equipped with a magnetic stir bar, dissolve 6 g of pork skin gelatin in 60 ml of phosphate buffered saline (PBS, pH=7.4), and stir to fully dissolve it at 50°C. Drop 0.72ml of methacrylic anhydride (MAA) into the gelatin solution at a rate of 0.1mL / min, and react with uniform stirring at 300rpm. Every 30min, adjust the...

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Abstract

The invention discloses thermosensitive hydrogel loaded with copper metal organic skeleton nanoparticles and a preparation method of the thermosensitive hydrogel. The preparation method comprises thesteps that an amino group on gelatin and methacrylic anhydride are subjected to acylation reaction to synthesize methylacrylic esterified gelatin with a branch containing a carbon-carbon double bond,the carbon-carbon double bond on the branch and N-isopropyl acrylamide are subjected to free radical polymerization, and methylacrylic esterified gelatin-g-poly N-isopropyl acrylamide is prepared; 1,3,5-benzene tricarbonic acid and copper acetate monohydrate are used for preparing copper-based MOF nanoparticles; the copper-based MOF nanoparticles are added to a methylacrylic esterified gelatin-g-poly N-isopropyl acrylamide solution, and a product is prepared. Copper ions are embedded in the biodegradable thermosensitive hydrogel in the form of HKUST-1 NPs, controlled slow release of the copperions is achieved, cytotoxicity is effectively reduced, migration of in-vitro dermal cells is promoted, angiogenesis is induced, and wound healing is promoted.

Description

Technical field [0001] The present invention relates to a medical dressing that can be used for tissue regeneration, in particular to a temperature-sensitive hydrogel loaded with copper metal organic framework nanoparticles and a preparation method thereof. The present invention carries copper ions in the form of copper metal organic framework nanoparticles (HKUST-1NPs). Into the temperature-sensitive hydrogel to achieve controlled release. Background technique [0002] As a hydrolysate of collagen, gelatin contains many arginine-glycine-aspartic acid (RGD) sequences that promote cell attachment, as well as matrix metalloproteinase (MMP) target sequences suitable for cell remodeling. Gelatin also promotes The advantages of macrophage growth and platelet coagulation. Poly-N-isopropylacrylamide is a typical temperature-sensitive hydrogel. Because of its simple polymerization method, stable properties, and the advantages of LCST near human body temperature, it has become an ideal m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L26/00
CPCA61L26/0004A61L26/0014A61L26/0038A61L26/0061A61L26/0066A61L26/008A61L26/009A61L2300/224A61L2300/412A61L2400/06C08L33/26
Inventor 彭晓宏马晓峰
Owner SOUTH CHINA UNIV OF TECH
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