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Hereditary deafness related gene detection chip kit

A gene detection chip and hereditary deafness technology, which can be used in the measurement/testing of microorganisms, biochemical equipment and methods, etc., and can solve the problems of low accuracy

Active Publication Date: 2019-03-22
上海伯豪生物技术有限公司
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Problems solved by technology

[0013] However, in actual use, we found that the detection accuracy of the existing kits using gene chips (microarray chips) for the detection of populations in southern China is much lower than that of populations in northern China

Method used

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  • Hereditary deafness related gene detection chip kit
  • Hereditary deafness related gene detection chip kit
  • Hereditary deafness related gene detection chip kit

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Embodiment Construction

[0127] The technical solutions of the present invention will be clearly and completely described below in conjunction with the accompanying drawings. Apparently, the described embodiments are part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0128] 1. Gene chip design

[0129] According to the detection target, a specific gene sequence is selected from the NCBI gene database as a probe; the probe sequence and its layout are designed according to the selected nucleic acid sequence, so that the designed probe combination has strong hybridization specificity for the gene to be tested Good correlation between needles, optimization of probe array and its spatial layout; its main advantage is that it can simultaneously detect 20 locus mutations on three hot...

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Abstract

The invention discloses a hereditary deafness related gene detection chip kit, which comprises a primer and a specific probe for detecting 20 site variation on three hotspot genes of hereditary deafness, wherein 20 sites comprise 5 mutant types on GJB2, 12 mutant types on SLC26A4 and 3 mutant types on 12S rRNA (MTRNR1, belonging to a chondriogene); the sequence of the specific probe is shown as SEQ ID NO: 1 to SEQ ID NO: 40; the sequence of the specific probe is shown as SEQ ID NO: 41 to SEQ ID NO: 60. The invention also discloses a concrete detection method. The kit is designed especially forthe hotspot mutation sites of the hereditary deafness gene of people groups of southern China; the detection accurate rate of the hereditary deafness of people groups of southern China can be improved; the kit overcomes the defects that the existing kit is mainly designed for the sites of people groups of Northern China, and the detection on the people groups of southern China is liable to leak detection.

Description

technical field [0001] The invention relates to the field of gene detection, in particular to a gene chip kit for detection of hereditary deafness-related genes aimed at hotspot mutations in southern Chinese populations. Background technique [0002] Deafness is a common hearing problem caused by auditory conduction pathway disorders caused by genetic or / and environmental factors. Existing studies have shown that about 60% of them are caused by genetic factors. Among hearing problems caused by genetic factors, about 70% of them are inherited in an autosomal recessive manner. Hereditary deafness is caused by mutations in a single gene or compound mutations in different genes. More than 140 deafness-related genes have been discovered so far, with high genetic heterogeneity of genes and loci. Through the detection and analysis of these genes, the inheritance mode can be determined, the risk genes carried can be scientifically assessed, scientific marriage and childbearing can...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/16C12Q2600/156
Inventor 张春秀陆孝亮刘立马晶晶曾圣曦肖华胜
Owner 上海伯豪生物技术有限公司
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