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A strain of Alternomonas with algicidal ability and its application to Prorocentrum donghaiense

A technology of alteromonas and algae dissolving, applied in the field of environmental microorganisms, can solve the problems of ineffective killing of Prorocentrum algae in the East China Sea, inability to fundamentally control red tide, negative impact on benthic organisms, etc., and achieve good algae dissolving effect , Environmentally friendly, easy to cultivate the effect

Active Publication Date: 2021-08-31
FUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the physical method is effective quickly and will not cause harm to the ecological environment, it is very likely to have a negative impact on benthic organisms and cannot fundamentally control red tides
The chemical method is currently the most effective method in comparison, but it is easy to cause secondary pollution to the environment, and it cannot only affect red tide organisms in a targeted manner. It will kill red tide organisms and also cause a certain degree of damage to non-red tide organisms. injury on
However, there is no report on the algae-dissolving bacteria and bacterial agents that can effectively kill Prorocentrum donghaiense

Method used

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  • A strain of Alternomonas with algicidal ability and its application to Prorocentrum donghaiense
  • A strain of Alternomonas with algicidal ability and its application to Prorocentrum donghaiense
  • A strain of Alternomonas with algicidal ability and its application to Prorocentrum donghaiense

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Experimental program
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Effect test

Embodiment 1

[0027] The screening of embodiment 1 algae-lytic bacteria

[0028] In the late stage of a Prorodinova red tide event in Xiapu, Fujian, surface seawater samples were taken, and the seawater samples were inoculated on 2216E solid medium by streaking on a plate, and cultured at 28°C for 48 hours. Inoculate each grown single colony in 1mL of 2216E liquid medium, culture at 28°C and 200rpm for 24 hours, take 0.5mL of bacterial liquid and inoculate it into 10mL of Prorocentrum donghaiense algal liquid in the logarithmic growth phase after aseptic treatment , After 48 hours, the strains that make the algae liquid appear yellow are considered as alginolytic bacteria. A bacterium with the best algicidal effect was selected for further research, and the bacterium was code-named FDHY-03.

[0029] Prorocentrum donghaiense culture conditions: Prorocentrum donghaiense ( Prorocentrum donghaiense Lu) was cultured in L1 medium and cultured in a light incubator at 20°C with a light intensit...

Embodiment 2

[0031] Embodiment 2 Alteromonas FDHY-03 strain identification

[0032] Morphological observation, physiological and biochemical reactions, staining and sugar fermentation experiments were carried out on the FDHY-03 strain. The results showed that the FDHY-03 bacteria had the following biological characteristics: rod-shaped, single, Gram staining was negative, and the colony was round and opaque. , the colony is beige, the surface is smooth and wet, and it is viscous when stirred up. Spore staining, methyl red (MR), V-P determination, urease, citrate, and phenylalanine deaminase reactions are negative, and contact enzymes, Starch hydrolysis, glucose, fructose, xylose, sucrose, lactose, galactose, and mannitol were positive (Table 1).

[0033] Table 1 Physiological and biochemical reaction results of FDHY-03 strain

[0034]

[0035] The 16s rRNA of the FDHY-03 strain was amplified, and the gene sequence was cloned and sequenced. The results showed that it was related to Al...

Embodiment 3

[0036] Example 3 Algae-dissolving effect of different concentrations of Alteromonas FDHY-03 on Prorocentrum donghaiense

[0037] Fermentation method of Alteromonas FDHY-03:

[0038] (1) Bacteria: adopt the above-mentioned Alteromonas FDHY-03;

[0039] (2) Slant culture: inoculate the strains in step (1) on a solid slant medium, and culture at 28°C for 24 hours;

[0040] (3) Primary culture: Take the cultivated slant under aseptic conditions, take a ring with an inoculation loop and place it in 50 mL liquid medium, and cultivate it for 24 hours at 28°C with a shaker at 200 rpm to obtain the primary culture liquid;

[0041] (4) Expansion culture: inoculate the primary culture liquid into 500mL liquid culture medium with an inoculum amount of 5%, culture at 28°C and shaker at 200rpm for 24 hours to obtain the expansion culture liquid;

[0042] (5) Fermentation culture: Inoculate the expanded cultured bacterial liquid into 2000 mL of fermentation liquid with an inoculum size of 5%...

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Abstract

The invention provides an altermonas strain with algae-dissolving ability and application thereof, belonging to the field of microbiology of harmful red tide treatment. The alginolytic bacteria named Alteromonas (A lteromonas macleodii ) FDHY‑03, which was deposited in the China Center for Type Culture Collection on December 14, 2018, with the deposit number: CCTCC NO: M 2018895. The bacterial agent made from this bacterium has a significant inhibitory and killing effect on Prorocentrum donghaiense, and the algae dissolving rate can reach more than 90% under 24-hour treatment conditions, which can be applied in the event of harmful algal blooms of this species. In addition, the present invention also relates to an immobilized bacterial powder containing the above-mentioned bacteria and a preparation method thereof, which are easy to operate, reusable and convenient to recycle. The invention provides a new idea for developing a low-cost, environment-friendly method for preventing and controlling harmful algal blooms.

Description

technical field [0001] The invention relates to the field of environmental microorganisms, in particular to an altermonas strain with algae-dissolving ability and its application. Background technique [0002] In the past two decades, with the continuous advancement of my country's coastal urbanization process, coastal industry, agriculture, aquaculture and tourism have developed rapidly, which has also led to the increasing eutrophication of coastal waters, and the resulting harmful algal blooms have occurred The frequency and scale also show a clear increasing trend. Dinoflagellates are the main species that form toxic and harmful red tides, among which Prorocentrum donghaiense is the most important cause of red tide outbreaks in my country. In recent years, with the further deterioration of the global climate and the coastal environment, especially the intensification of coastal eutrophication and the increase of carbon dioxide concentration and water temperature in seawa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
CPCC02F3/34C12N1/20C12N1/205C12R2001/01
Inventor 石新国陈剑锋谢友坪刘乐冕郑向南马瑞娟
Owner FUZHOU UNIV
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