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A Ferulic Esterase-Producing Bacillus xievasan and Its Application in Liquor Daqu

A technology of ferulic acid esterase and Pseudomonas sheva, applied in the preparation of alcoholic beverages, fungi, microorganism-based methods, etc., can solve the problem of no production of ferulic acid esterase and the like

Active Publication Date: 2021-09-10
SHANDONG BANDAOJING +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] At present, some researches have shown that Trichomonas scheva can produce cellulase, amylase, and protease, but there is no report about its production of ferulic acid esterase. , Cladosporium, etc. can produce ferulic acid esterase, such as patent CN 102286442A research shows that Aspergillus fumigatus ferulic acid esterase activity is 61.89mU / mL, Aspergillus niger ferulic acid esterase activity is 53.26mU / mL, rice Aspergillus ferulic acid esterase enzyme activity is 0.78mU / mL

Method used

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  • A Ferulic Esterase-Producing Bacillus xievasan and Its Application in Liquor Daqu
  • A Ferulic Esterase-Producing Bacillus xievasan and Its Application in Liquor Daqu
  • A Ferulic Esterase-Producing Bacillus xievasan and Its Application in Liquor Daqu

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Experimental program
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Embodiment 1

[0029] Embodiment 1 strain identification

[0030] Bacterial strain M6 of the present invention is isolated from the middle and high temperature Daqu of Shandong Daodaojing Co., Ltd., and the strains have been preserved in the China General Microorganism Culture Collection Management Center (CGMCC No. 14125) and the China Industrial Microbiology Culture Collection Management Center (CICC 41584) ).

[0031] (1) Morphological identification

[0032] The strain M6 was inoculated on the yeast extract medium (CYA medium), cultured at 25°C for 7 days, colonies were photographed, microscopic observation of the bacteria and scanning electron microscopy were observed. For details of the morphological characteristics, seefigure 1 .

[0033] (2) Molecular biological identification

[0034] Strain M6 was accurately identified as a species through the phylogenetic analysis of ITS rDNA and β-tubulin gene sequence, and the phylogenetic tree is shown in Figure 2(a-b).

Embodiment 2

[0035] The research of embodiment 2 strains producing ferulic acid esterase

[0036] (1) Qualitative detection of enzyme activity

[0037] Inoculate the strains M1, M6, and M9 of S. chevalensis isolated from Baijiu Daqu of Shandong Daowaojing Co., Ltd. on a Chapei agar-based plate containing ethyl ferulic acid [0.01g FeSO4 7H2O, 2.0g NaNO3, 0.5g KCl, 0.5gMgSO4·7H2O, 1.0g K2HPO4, 30g sucrose, 20g agar, 0.1g rhodamine B, 1L distilled water, natural pH value, 1000mL medium cooled to 50-60℃, add 10mL containing 10 % ethyl ferulate in dimethylformamide solution (0.22 μm membrane filtration)], cultivated at 28°C for 5 days, if a transparent circle appears around the colony, it indicates that the bacterial strain can produce ferulic acid esterase, according to the transparent circle Judging the enzyme-producing ability of the bacterial strain by the size, the bacterial strain M6 with higher ferulic acid esterase activity was screened out (see image 3 ).

[0038] (2) Quantitative ...

Embodiment 3

[0044] Medium and high temperature Daqu intensive test of embodiment 3 Xiewa Sanyssa bacterial strain M6

[0045] (1) Strengthening method and sampling method

[0046] Get the prepared Xiewasan cyst bacteria powder 100g and dissolve it in 15L of distilled water containing 2%NaCl, and strengthen the middle and high temperature Daqu (numbering is respectively 19# spraying) by spraying and mixing two kinds of modes , 19# mixing material), each piece of Daqu is distributed to about 8-9mL with a final concentration of 1.2×10 5 For the CFU / mL bacterial solution, select Daqu without fortification of S. schevascens as the control (the numbers are respectively 19# control). The sampling method is to take a well-growing piece of koji from each of the four corners and the middle 5 positions of the koji room, crush it and mix it evenly, and sample it by quartering.

[0047] (2) Strengthen the sensory indicators of Daqu

[0048] According to the enterprise standard "Daqu Scoring Standar...

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Abstract

The invention relates to a ferulic acid esterase-producing fungus xievasan and its application in the production of liquor daqu, which belongs to the field of biotechnology. Sheva Sanga of the present invention ( Eurotium chevalieri ) is isolated from Baijiu Daqu, which has the characteristics of producing ferulic acid esterase. The original number of the strain is M6, and the strain preservation number is CGMCC No.14125. The present invention has the advantages of: providing a ferulic esterase-producing strain of Trichomonas xiewa, and preparing it into a fungus powder of the fungus, which is applied in a certain proportion to the production of liquor Daqu, High-quality Daqu with high ferulic acid esterase activity can be produced. The heart of Daqu has obvious golden plaques of Sancystis scheva. The wine fermented from this Daqu has higher alcohol content, caproic acid and caproic acid Features such as high ethyl ester content.

Description

technical field [0001] The invention relates to a ferulic acid esterase-producing fungus xievasan and its application in liquor daqu, which belongs to the field of biotechnology. Background technique [0002] Liquor Daqu is one of the traditional brewing koji, also known as block koji or brick koji, which is a crucial element in the fermentation production of liquor. It uses wheat, barley, peas, etc. as raw materials, which are crushed, mixed with water, and pressed into koji Unstrained spirits, shaped like bricks. According to different production temperatures, Daqu can be divided into low-temperature koji, medium-temperature koji, medium-to-high-temperature koji, and high-temperature koji; during the process of making koji, microorganisms grow in large numbers, and the macromolecular substances (starch, lipid, protein, etc.) ) is degraded into absorbable small molecular substances, which provide abundant substrates, microorganisms, and biological enzymes for later ferment...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12G3/022C12R1/645
CPCC12G3/02C12N1/145C12R2001/645
Inventor 赵纪文姚粟白秀彬程池许玲白飞荣翟磊于盼盼董乔娟于学健石鲁博
Owner SHANDONG BANDAOJING
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