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Paenibacillus polymyxa, application and microbial agent, powder and granule

A technology of polymyxa spore-like and microbial inoculum, which is applied in the fields of polymyxo-paenoid, application and microbial inoculum, powder and granule, can solve the problem of being easily affected by the natural environment, limited competitive survival ability, and single acting object, etc. question

Active Publication Date: 2021-06-08
河北福赛农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, most of the currently known Paenibacillus polymyxa can prevent and treat plant fungal diseases with poor effects, relatively single action objects, easy to be affected by the natural environment, and limited competitive survival ability.

Method used

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  • Paenibacillus polymyxa, application and microbial agent, powder and granule
  • Paenibacillus polymyxa, application and microbial agent, powder and granule
  • Paenibacillus polymyxa, application and microbial agent, powder and granule

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Isolation of L10 strain

[0028] After collecting well-grown corn and rinsing the stalks with clean water, the following operations are completed in an ultra-clean workbench. Cut corn stalks with a length of 2 cm with sterile scissors, soak the corn stalks in 95% alcohol for 5 minutes, then disinfect them with 10% sodium hypochlorite for 5 minutes, wash them with sterile water for 4 times, air dry them in a sterile environment, and put them in In a sterile mortar, grind to make the interstitial fluid flow out, filter, and dilute to 10mL with sterile water, let it stand for 10min, and dilute according to the dilution to obtain 10 -1 , 10 -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 , 6 concentration gradients, and then take 0.2mL of the bacterial suspension of each concentration gradient on the plate, then pour 15mL of LB solid medium at 50°C into the plate, shake the plate, repeat the above steps for 3 times for each gradient The second time, culture statically a...

Embodiment 2

[0029] Embodiment 2: Screening of antagonistic strains

[0030] Using Fusarium graminearum as the pathogenic bacteria, inoculate the plate in the center of the PDA plate. Using the plate confrontation method, the isolated and purified Paenibacillus polymyxa L10 strain was streak-inoculated at a distance of 3 cm from the disc. Only Fusarium graminearum was inoculated in the blank control. Three control groups were inoculated according to the above inoculation method, cultured in a dark environment at 25°C for 7 days, and the antagonistic effect was observed. The antibacterial effect is determined according to the size of the inhibition zone, and the strains with a diameter of 1.0cm>inhibition zone>0.5cm are defined as antagonistic strains; those with a diameter of 1.5cm>inhibition zone>1.0cm are regarded as antagonistic bacteria, and the diameter of inhibition zone> 1.5 cm has a better antibacterial effect as an antagonistic bacterium, and the bacterial strain with the best a...

Embodiment 3

[0031] Example 3: Sequence determination and analysis of 16S rDNA of bacterial strain and identification of physiological and biochemical tests

[0032]Paenibacillus polymyxa DNA was extracted using bacterial genomic DNA extraction kit (Ezup column type), and bacterial universal primers were used to amplify the 16S rDNA sequence. -3'). The PCR reaction system is (50 μL) 1 μL of each primer, 2 μL of DNA template, 25 μl of 2×Mix, and 21 μl of ultrapure water. The PCR amplification program was 94°C for 3min, 94°C for 1min, 52°C for 1min, 72°C for 1.5min, 30 cycles, 72°C for 10min, 16°C for 10min. The amplified products were sent to Shanghai Sangon Biotechnology Co., Ltd. for sequencing.

[0033] The measured 16S rDNA sequence was input into GenBank, and BLAST software was used for homology search. The 16S rDNA sequences of different strains were selected and compared with the known 16S rDNA sequences in GenBank.

[0034] The same-origin comparison results are as follows:

[...

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Abstract

The invention provides a Paenibacillus polymyxa, application and microbial agent, powder and granule, which are isolated from corn stems, and the strain is effective against various plant fungal diseases, especially Fusarium graminearum and Fusarium laminar fungal diseases such as Verticillium spp. or Fusarium pseudoverticillium have good control effects, do not pollute the environment, are ecologically safe, genetically stable, and are not easy to produce resistance; the present invention can be mixed with organic and inorganic nutrients required for plant growth to prepare microorganisms Bacteria root irrigation, powder seed dressing and granules can significantly enhance the ability of crops to resist fungal diseases.

Description

technical field [0001] The invention relates to the technical field of microbial application, in particular to Paenibacillus polymyxa, its application and microbial agent, powder and granule. Background technique [0002] About 70% of plant diseases are caused by fungi. Plants may be infected by fungi at any stage of growth, especially food crops. Once infected by plant pathogenic fungi, they may produce a variety of toxins, which will eventually endanger human health. . With the use of pesticides, the resistance of plant fungi has increased, leading to fungal blooms. The use of plant endophytes in biological control can reduce the use of chemical pesticides, thereby reducing environmental pollution, and plant endophytes play an important role in the ecological internal environment, which has attracted the attention of scholars. The use of beneficial microorganisms to prevent and control plant diseases began in 1921, when Hartely used fungi to prevent and control plant dis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N25/12A01N63/25A01P3/00C12R1/01
CPCA01N25/12A01N63/10C12N1/20C12N1/205C12R2001/01
Inventor 郝志敏王会敏董金皋梁海龙申珅曾凡力
Owner 河北福赛农业科技有限公司
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