Chromone derivative extracted from Cassia pumila as well as preparation method and application of chromone derivative

A technology of Lentil adenophora, derivatives, applied in the field of phytochemistry

Active Publication Date: 2019-03-08
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The present invention has isolated a kind of chromone derivative with anti-tobacco mosaic virus activity from P.

Method used

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  • Chromone derivative extracted from Cassia pumila as well as preparation method and application of chromone derivative
  • Chromone derivative extracted from Cassia pumila as well as preparation method and application of chromone derivative
  • Chromone derivative extracted from Cassia pumila as well as preparation method and application of chromone derivative

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preparation example Construction

[0026] The preparation method of the chromone derivative 5-(isobutyryl)-2-(2-oxopropyl)-8-methoxy-4H-4-chromone of the present invention comprises crushing, extracting extract, silica gel Column chromatography, high pressure liquid chromatography and gel column chromatography steps, specifically include:

[0027] A, extraction of extract: get the whole plant of lentil lentils, lentils lentils can be crushed or cut into small sections, with high-concentration methanol (w%: 80% ~ 100%) or high-concentration ethanol (w%: 80%) %~100%) or high-concentration acetone (w%: 70%~100%) is the extraction solvent, extraction solvent: three tendons of hairy leaves (weight ratio)=2~4:1, soak for 24h~72h, extract 3~5 times, the extracts were combined, filtered and concentrated into an extract;

[0028]B. Silica gel column chromatography: the extract is dissolved with pure methanol or pure ethanol or pure acetone with a weight ratio of 1.5 to 3 times, and then mixed with 80 to 100 mesh silica...

Embodiment 1

[0034] The lentil lentils sample comes from Xinping County, Yunnan. 2.0 kg of the lentil lentils sample was crushed and extracted 5 times with 95% methanol for 24 hours each time. The extracts were combined, filtered, and concentrated under reduced pressure to obtain an extract. 106g. The extractant is dissolved in pure methanol with a weight ratio of 2.0 times, and then mixed with 120 g of 80 mesh thick silica gel, and 0.7 kg of 160 mesh silica gel is packed for silica gel column chromatography, and the volume ratio is 20:1, 9:1, 8:2, 7:3, 6:4, 5:5 chloroform-methanol gradient elution, TLC monitoring and merging the same fractions to obtain 6 fractions, among which the chloroform-methanol elution fraction with a volume ratio of 9:1 Separation by Shimadzu LC-8A semi-preparative high performance liquid chromatography with 55% methanol as mobile phase, Venusil MP C 18 (20×250mm, 5μm) The preparative column is a stationary phase, the flow rate is 20ml / min, the detection waveleng...

Embodiment 2

[0036] The sample of Shandenshan lentils comes from Shuangjiang County, Yunnan Province. Take 4.0kg of Bandenshan lentils and chop them up, extract them with 95% ethanol for 4 times, each time for 48 hours, combine the extracts, filter, and concentrate under reduced pressure to form an extract. Obtain extractum 196g. After dissolving the extract with 2.0 times the amount of pure methanol by weight, mix the sample with 200g of 100-mesh silica gel, pack 1kg of 200-mesh silica gel into a column for silica gel column chromatography, and use a volume ratio of 20:1, 9:1, 8 :2, 7:3, 6:4, 5:5 chloroform-methanol gradient elution, TLC monitoring and merging the same parts to obtain 6 parts, wherein the chloroform-acetone elution part with a volume ratio of 9:1 was used Shimadzu's LC-8A semi-preparative high-performance liquid chromatography separation, with 58% methanol as the mobile phase, Venusil MP C 18 (20×250mm, 5μm) The preparative column is a stationary phase, the flow rate is ...

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Abstract

The invention discloses a chromone derivative extracted from Cassia pumila as well as a preparation method and application of the chromone derivative. The chromone derivative is separated from complete Cassia pumila, the molecular formula of the chromone derivative is C17H18O5, and a compound of the chromone derivative is 5-(isobutyryl)-2-(2-oxo-propyl)-8-methoxy-4H-4-chromone. The preparation method of the chromone derivative comprises the following steps: taking the complete Cassia pumila as a raw material, smashing the complete Cassia pumila, extracting the smashed complete Cassia pumila and performing slica column chromatography and HPLC separation. Through a test for biological activity of the compound, the compound has a good effect of inhibiting a tobacco mosaic virus. The compoundis easy to separate, is good in activity and can serve as a guiding compound for resisting the tobacco mosaic virus.

Description

technical field [0001] The invention belongs to the technical field of phytochemistry, and in particular relates to a chromone derivative extracted and separated from lentil lentils and its preparation method and application. Background technique [0002] Cassiapumila Lam. is a perennial subshrub-like diffuse herb of the genus Cassia of the Leguminosae family. It is also known as Mimosa cassia, cucumber fragrance, dream grass, tart sand, thin bar wood, sand grass, Cypress grass, saponins, lignified base, 25-75 cm high, multi-branched, linear sickle-shaped, with small protuberances at the top, branches, branches, petioles, and leaf rachis sparsely pilose, flowering in August-September ;October-December results. Phyllostachys lenticans grows in bushes or grasses in mountains and open areas. It is mainly distributed in Guangdong, Yunnan and other provinces in China. It is also distributed in India, Indochina Peninsula, Malaysia, and Australia abroad. As a traditional Chinese h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/22A01N43/16A01P1/00
CPCA01N43/16C07D311/22
Inventor 李银科黄茹李雪梅杨光宇刘欣杨叶昆黄海涛李晶徐永张建铎
Owner CHINA TOBACCO YUNNAN IND
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