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Detection method for detecting agilawood quality by using high-performance liquid chromatography

A technology of high-performance liquid chromatography and a detection method, which is applied in the detection field of high-performance liquid chromatography to determine the quality of agarwood, can solve the problems of low accuracy of detection results, one-sided quality evaluation of agarwood medicinal materials, etc., and achieve prolonged storage time and good accuracy , High detection efficiency

Inactive Publication Date: 2019-03-01
北京三和药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, in the Pharmacopoeia, only the quality control index of agarwood is used to measure the quality of agarwood, which makes the quality assessment of agarwood medicinal materials relatively one-sided, and the accuracy of the test results obtained is low.

Method used

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  • Detection method for detecting agilawood quality by using high-performance liquid chromatography
  • Detection method for detecting agilawood quality by using high-performance liquid chromatography
  • Detection method for detecting agilawood quality by using high-performance liquid chromatography

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0085] ②. Preparation of the mother liquor of the reference substance. Accurately weigh 0.30 mg of agarotetrol, 0.30 mg of 6-hydroxy-2-[2-(4′-methoxy)phenethyl]chromone, and 0.30 mg of 6- Hydroxy-2-(2-phenylethyl)chromone, 0.30mg of 6,7-dimethoxy-2-[2-(4'-methoxy)phenethyl]chromone and 0.30mg of 6 , 7-dimethoxy-2-(2-phenylethyl) chromone, the five chromones, were dissolved in methanol after filtering through a 0.22 μm microporous membrane and degassed, and the volume was adjusted to 5ml, and sealed and stored Store in a refrigerator at 4°C for later use, then take 1ml and mix them to form 1ml of a mixed solution containing 60μg of chromone, and filter through a 0.22μm microporous membrane to obtain the mother solution of the reference substance;

[0086] ③. Sample analysis

[0087] ③-1. Sample pretreatment: Grind agarwood, take an appropriate amount of agarwood powder passed through a 40-mesh sieve, accurately weigh 1.00mg, add 50ml of methanol that has been filtered through ...

Embodiment 2

[0104] The difference from Example 1 is that in step ① of this example, the column temperature is 30°C; the mobile phase flow rate is 0.5ml / min; the linear gradient is:

[0105] 0-15min: 80% of phase A + 20% of phase B;

[0106] 16-30min: 65% of phase A + 35% of phase B;

[0107] 31-80min: 60% phase A + 40% phase B;

[0108] 81-90min: 10% phase A + 90% phase B;

[0109] In step ③ of this embodiment, the amount of methanol added is 30ml, and the extraction time of the agarwood powder by methanol is 0.8h.

Embodiment 3

[0111] The difference from Example 1 is that in this example, in the chromatographic conditions of step ①, the column temperature is 38°C; the mobile phase flow rate is 1.0ml / min; the linear gradient is:

[0112] 0-15min: 82% of phase A + 18% of phase B;

[0113] 16-30min: 70% of phase A + 30% of phase B;

[0114] 31-80min: 65% of phase A + 35% of phase B;

[0115] 81-90min: 10% phase A + 90% phase B;

[0116] In step ③ of this embodiment, the amount of methanol added is 60ml, and the extraction time of the agarwood powder by methanol is 1.2h.

[0117] 2.4, Embodiment 4

[0118] The difference from Example 1 is that in this example, in the chromatographic conditions of step ①, the column temperature is 35°C; the mobile phase flow rate is 0.8ml / min; the linear gradient is:

[0119] 0-15min: 85% of phase A + 15% of phase B;

[0120] 16-30min: 65% of phase A + 35% of phase B;

[0121] 31-80min: 65% of phase A + 35% of phase B;

[0122] 81-90min: 10% phase A + 90% phase B; ...

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Abstract

The invention belongs to the technical field of agilawood quality detection, and particularly discloses detection method for detecting agilawood quality by using a high-performance liquid chromatography. The method comprises the operation steps of chromatographic condition setting, preparation of a mother liquor of a reference substance, sample analysis and calculation for the analysis result. Byadopting the detection method, five chromones including agarotetrol, 6-hydroxy-2-[2-(4'-methoxyl) phenethyl] chromone, 6-hydroxy-2-(2-phenethyl) chromone, 6,7-dimethoxy-2-[2-(4'-methoxyl) phenethyl] chromone and 6,7-dimethoxy-2-(2-phenethyl) chromone which are contained in agilawood can be detected at a time, the comprehensiveness of agilawood quality detection is effectively improved, the accuracy of detecting the agilawood quality is improved, and the method has the advantages that the operation is simple, and the detection efficiency is high.

Description

technical field [0001] The invention belongs to the technical field of agarwood quality detection, and in particular relates to a detection method for determining the quality of agarwood by high performance liquid chromatography. Background technique [0002] Agarwood is a resinous wood of Aquilaria sinensis (Lour.) Gilg, a plant of the family Thymelaeaceae, which is mainly distributed in China, Japan, India, the Middle East, Southeast Asia and other countries. Currently, there are 19 species of Aquilaria in the family Thyrrhaceae, but only 4 of them can produce agarwood, which are Aquilaria malaccensis, Aquilaria crassna, Aquilaria sinensis and Aquilariafilaria. ). [0003] As a traditional Chinese medicine, agarwood has been used for a long time in my country. It is mainly used clinically to treat pulmonary heart disease, coronary heart disease, cerebral hemorrhage, stomach cold, asthma and other diseases. The chemical components in agarwood mainly include volatile oil, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 郭学东
Owner 北京三和药业有限公司
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