A kind of antimicrobial peptide for preventing and treating small melon worm
A technology of antimicrobial peptides and melon worms, applied in the direction of peptides, peptide sources, anti-infective drugs, etc., can solve the problems of affecting fish respiration, strong toxicity, carcinogenicity, etc., achieve good killing effect, good stability, and peptide sequence short effect
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Embodiment 1
[0015] Development and preparation of embodiment 1 antimicrobial peptide YcHβ
[0016] The skin of fish has been soaked in the water environment containing various bacteria and parasites for a long time. For the scaleless yellow catfish, this layer of mucus covering the body surface is particularly important for resisting infection by exogenous pathogens. We infect the yellow catfish with small melon worms and collect the surviving yellow catfish mucus before and after immunization, then use a freeze dryer to dry the mucus into powder in a vacuum freezing environment, and find the yellow catfish after immunization after dissolving in water The mucus has a killing effect on the trophozoites of the small melon worm. The proteome of the yellow catfish before and after immunization was measured by LC-MS / MS technology, and the proteins that were significantly up-regulated were found. The complete CDs sequence of the corresponding protein was found through the transcriptome and gen...
Embodiment 2
[0018] The control effect of embodiment 2 antibacterial peptide YcHβ to small melon worm
[0019] 1. Materials and methods
[0020] 1.1 Test fish
[0021] The yellow catfish severely infected with the small melon worm was mixed with the normal yellow catfish (28.5±6.7g) in a 60L water tank and inoculated with the small melon worm. 20 fish were randomly selected, and the white cysts on the body surface were clearly visible. The infection rate of the test fish was 100%.
[0022] 1.2 Preparation of trophozoites, cysts and predators
[0023] When the yellow catfish is severely infected by small melon worms (many white spots can be seen with the naked eye on the surface of the fish body), gently use a cell scraper to scrape off the white bag of the fish body bag and collect it in a small beaker, rinse it repeatedly with distilled water for several times until Trophozoites are formed after most of the mucus has been removed. Transfer the small melon worms to another petri dish f...
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