Polypeptide for inhibiting degradation of nitric oxide synthase and hypertension and application thereof
A nitric oxide, enzymatic degradation technology, applied in the fields of peptides, drug combinations, cardiovascular system diseases, etc., can solve problems such as activity reduction, and achieve the effect of short sequence, broad application prospects, and widening selection fields.
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[0025] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments and accompanying drawings.
[0026] source of biological material
[0027] 1) TAT-PKPREPFD, TAT-PKPREPFD-Rhodamine B, and TAT-PKPREPFD-Flag were all synthesized by Beijing Zhongke Yaguang Biotechnology Co., Ltd.; 2) Fresh and healthy newborn umbilical cords were obtained from the Obstetrics and Gynecology Department of the First Affiliated Hospital of Sun Yat-sen University; 3) African green monkey kidney fibroblast cell line Cos7 was imported from ATCC in the United States and kept in our laboratory; 4) eNOS-GFP plasmid was provided by Professor Kong Deling of Nankai University in Tianjin; 5) C57BL / 6 mice were purchased from the Experimental Animal Center of Sun Yat-sen University.
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