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GTR (guided tissue regeneration) membrane and preparation method thereof

A technology that guides tissue regeneration and loosens pores. It can be used in surgery, medical science and other directions. It can solve the problems of complex production process, high price, and inability to meet the requirements, and achieve the effect of simple operation steps, good biocompatibility and mild reaction system.

Active Publication Date: 2019-03-01
江苏博创生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, domestic and foreign products have defects such as complex production process, high price and single structure and function, which can no longer meet the requirements of tissue regeneration for membrane materials.

Method used

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  • GTR (guided tissue regeneration) membrane and preparation method thereof
  • GTR (guided tissue regeneration) membrane and preparation method thereof
  • GTR (guided tissue regeneration) membrane and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Example 1: Preparation of a structurally asymmetric guided tissue regeneration membrane with osteogenic activity

[0088] (1) Configuration of chitosan solution:

[0089] Weigh 10g of chitosan powder in 900ml of ultrapure water and stir to disperse evenly, then add 32mL of 1.2M dilute hydrochloric acid solution, and set the volume to 1000mL. Stir overnight to completely dissolve, dilute hydrochloric acid to adjust the pH to between 4.5-5.5, centrifuge at 7500rpm / min at 4°C to remove insoluble impurities and obtain a 1% chitosan solution for subsequent use.

[0090] (2) Configuration of chitosan-nanometer hydroxyapatite composite solution:

[0091] Weigh 400 mg of nano-hydroxyapatite powder and add it into 100 ml of the chitosan solution described in step (1), stir overnight until completely dispersed and set aside.

[0092] (3) Preparation of chitosan / chitosan-nano hydroxyapatite asymmetric bilayer membrane:

[0093] Before film formation, add 25 μl / ml of hydrogen pe...

Embodiment 2

[0097] Example 2: Physicochemical characterization of structurally asymmetric guided tissue regeneration membrane with osteogenic activity

[0098] The Fourier total reflection infrared spectrum and X-ray diffraction spectrum of CS / CS-nHAps double-sided figure 2 shown in A.

[0099] Compared with the CS surface, the CS-nHAps surface after adding nHAps appeared on the infrared spectrum at 573cm -1 、601cm -1 and 1084cm -1 PO 3- 4 The characteristic absorption peak and stretching vibration peak of the bending deformation also appeared at 1590cm -1 、1562cm -1 and 1658cm -1 The characteristic absorption peaks of -NH2 and C=O.

[0100] At the same time, if figure 2 On the X-ray spectrum shown in B, a series of characteristic diffraction peaks of nHAps appeared in the CS-nHAps layer, indicating that the loose porous layer is a composite layer of chitosan and nano-hydroxyapatite.

Embodiment 3

[0101] Example 3: Morphological characterization of a structurally asymmetric guided tissue regeneration membrane with osteogenic activity

[0102] After the CS / CS-nHAps asymmetric double-layer film was vacuum-dried for 24 hours, the loose surface and the dense surface of the sample were respectively pasted on the copper platform. Loose porous surface and dense surface morphology (accelerating voltage is 20kV), such as image 3 shown.

[0103] The loose and porous surface is a three-dimensional network structure with a thickness of 450±30 μm. The pores are interconnected and regular. The average pore size is about 390 μm and the porosity is 90±2%. It is beneficial to the absorption of nutrients and the discharge of metabolic waste, as well as the proliferation and differentiation of osteoblasts.

[0104] The dense surface is relatively flat and smooth, with a thickness of 82±6μm, no obvious pore structure, and a porosity of 5±1%. It will play a certain barrier effect on fib...

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Abstract

The invention discloses a GTR (guided tissue regeneration) membrane and a preparation method thereof. The GTR membrane comprises a compact layer with a chitosan matrix and a loose porous layer with achitosan and calcium phosphate compound matrix. A three-electrode system is adopted, compacted chitosan hydrogel is firstly deposited near cathodes, then, loose chitosan and calcium phosphate hydrogelis deposited on the chitosan hydrogel, double-layer hydrogel is obtained, and the GTA membrane is obtained after drying. The loose porous layer of the GTA membrane has osteogenic activity and can provide more structural space for cell growth and good microenvironment for proliferation and differentiation and promote fusion of bone cells, and meanwhile, the compact layer can shield fibrous tissue.The preparation method is simple, rapid, green in process and controllable for membrane structure.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a guided tissue regeneration membrane and a method for rapidly preparing a structurally asymmetric guided tissue regeneration membrane with osteogenic activity. Background technique [0002] Guide Tissue Regeneration (GTR) is a routine clinical repair method for periodontal tissue. The principle is to create an environment for bone tissue to grow preferentially according to the characteristics of different migration speeds of various tissue cells, that is, to place the barrier membrane Establish a biological barrier between soft tissue and bone defect, create a relatively closed tissue environment, prevent fibrous connective tissue cells and epithelial cells that interfere with bone formation and migrate faster into the bone defect area, and allow potential growth and faster migration. The slow precursor osteoblasts preferentially enter the bone defect area and gro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L31/12A61L31/04A61L31/02A61L31/14A61L31/16
CPCA61L31/028A61L31/042A61L31/145A61L31/146A61L31/148A61L31/16A61L2300/412C08L5/08
Inventor 刘昌胜屈雪雷淼
Owner 江苏博创生物科技有限公司
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