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Application of PC2 gene or recombinant virus expressing PC2 gene to preparation of medicament for treating osteoarthritis

A technology for osteoarthritis and recombinant virus, applied in gene therapy, drug combination, antipyretics, etc., can solve the problems of cartilage damage and repeated use

Active Publication Date: 2019-03-01
INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Direct injection of drugs into the articular cavity can quickly eliminate inflammatory stimuli, block the occurrence and development of pathological reflexes, relieve pain, and promote the healing and regeneration of cartilage, but the injection itself may damage cartilage, so it is not suitable for repeated use

Method used

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  • Application of PC2 gene or recombinant virus expressing PC2 gene to preparation of medicament for treating osteoarthritis
  • Application of PC2 gene or recombinant virus expressing PC2 gene to preparation of medicament for treating osteoarthritis
  • Application of PC2 gene or recombinant virus expressing PC2 gene to preparation of medicament for treating osteoarthritis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1, preparation of recombinant lentivirus

[0037] The PC2 protein is shown as sequence 1 in the sequence listing. The PC2 gene is shown as sequence 2 in the sequence listing.

[0038] The pLE4-GFP plasmid is a circular plasmid shown in sequence 3 of the sequence listing.

[0039] 1. Construction of recombinant plasmids

[0040] By means of homologous recombination, the small fragment between the XhoI and MluI restriction sites of the pLE4-GFP plasmid is replaced with the DNA molecule shown in Sequence 2 of the sequence table, and the recombinant lentiviral plasmid expressing the PC2 gene is obtained, also known as lentiviral plasmid Viral plasmid pLE4-PC2.

[0041] 2. Preparation of PC2 virus solution

[0042] (1) Using Lipo3000 transfection kit (ThermoFisher), co-transfect 293T cells with lentiviral plasmid pLE4-PC2, plasmid psPAX2 and plasmid pMD2G (ratio: each cell culture dish with a diameter of 10 cm is transfected with 15 μg of lentivirus Plasmid p...

Embodiment 2

[0048] Example 2, Therapeutic effect of PC2 gene on osteoarthritis

[0049] SPF grade C57BL / 6N mice, male, 10 weeks old, weighing 20±2g, were purchased from Huafukang Company. Raising conditions for mice: 23°C, 12 hours of light / 12 hours of darkness, free access to food and water.

[0050] Step 1, Step 2 and Step 3 are counting days consecutively.

[0051] 1. Establishment of a mouse model of osteoarthritis induced by cutting the anterior cruciate ligament

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Abstract

The invention discloses application of a PC2 gene or a recombinant virus expressing the PC2 gene to preparation of a medicament for treating osteoarthritis. The invention provides application of the PC2 gene to the preparation of a medicament for treating osteoarthritis. The PC2 gene is a gene coded with a PC2 protein.The PC2 protein is a protein consists of an amino acid sequence shown as a sequence 1 in a sequence table. As proved by the inventor of the invention, the PC2 gene has a definite treatment effect on osteoarthritis, so that a new thought is provided for the development of a gene applied to osteoarthritis treatment, and the selection range of clinical gene treatment is enlarged. The PC2 gene has important application and popularization value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of PC2 gene or recombinant virus expressing PC2 gene in the preparation of osteoarthritis treatment medicine. Background technique [0002] Osteoarthritis (OA) is a common degenerative bone and joint disease, which is caused by damage to the integrity of cartilage in joints and lesions of subchondral bone plates, resulting in joint symptoms and symptoms. The clinical manifestations of osteoarthritis are joint pain, stiffness, and motor dysfunction, which can affect the quality of life of patients in severe cases. Its pathogenesis is still unclear. It is generally believed that biomechanical factors, genetic factors, and environmental factors are involved in the metabolic changes of synovium, cartilage, and bone. Mucopolysaccharides in the cartilage matrix are reduced, cartilage is degraded, and inflammation occurs. [0003] At present, the treatment metho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61P19/02A61P29/00A61P19/08
CPCA61K48/005A61P19/02A61P19/08A61P29/00
Inventor 曲静刘光慧任晓庆任若通
Owner INST OF ZOOLOGY CHINESE ACAD OF SCI
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