Experiment method for preventing melanoma through nature killer (NK) cells

A technology of NK cells and experimental methods, applied in animal experiments to study the effect of NK cells on the prevention of melanoma, NK cells in the field of melanoma prevention, can solve the problems of unsatisfactory clinical immunotherapy effects, NK cell defects, etc.

Inactive Publication Date: 2019-03-01
TIANJIN KANGTING BIOLOGICAL ENG GRP CO LTD
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  • Claims
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AI Technical Summary

Problems solved by technology

Most clinical studies use CD56 isolated from the peripheral blood of cancer patients + , CD3 - NK cells, and then expanded and cultured in vitro, and then reinfused into the patient, but because most patients' NK cells are defective, the clinical effect of immunotherapy is not ideal

Method used

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  • Experiment method for preventing melanoma through nature killer (NK) cells
  • Experiment method for preventing melanoma through nature killer (NK) cells
  • Experiment method for preventing melanoma through nature killer (NK) cells

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Embodiment Construction

[0023] The present invention will be further described in detail below in conjunction with the accompanying drawings and through specific embodiments. The following embodiments are only descriptive, not restrictive, and cannot limit the protection scope of the present invention.

[0024] The present invention has done following innovation in experimental method:

[0025] 1. Selection of immune cells and tumor cell types;

[0026] 2. NK cells are used to prevent melanoma;

[0027] 3. NK cell administration method and dosage;

[0028] 4. In order to better illustrate that NK cells can enhance the immunity of mice and improve the activity of immune cells in mice, the present invention chooses to subcutaneously implant tumor cells 1 week after NK cells stop, each 2×10 6 cells around.

[0029] The specific process is described as follows:

[0030] An experimental method for NK cells to prevent the occurrence of melanoma in BALB / C nude mice, the method is composed of the followi...

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Abstract

The invention relates to an experiment method for preventing melanoma through nature killer (NK) cells. The experiment method relates to the following innovations of selecting of the types of immune cells and tumor cells, application of the NK cells to prevent the melanoma and the administration mode and the dosage of the NK cells, and relates to the following innovation that in order to better show that the NK cells can enhance mouse immunity and improve the activity of the immune cells in mice, after the NK cells are stopped in one week, subcutaneous implantation of the tumor cells is carried out, and each mouse is subjected to subcutaneous implantation of the 2*106 cells. In-advance back transfusion of the NK cells is adopted, thus a method for preventing the melanoma is achieved, the biological characteristics of the NK cells for killing tumors and improving and stimulating autoimmunity are utilized to the maximum extent, thus the tumors are prevented, and the NK cells exert the more obvious and more stable effects advantageously in later research and conical application. The experiment method is simple in step and outstanding in effect, and has certain significance to pre-clinical research in cancer prevention of the NK cells.

Description

technical field [0001] The invention belongs to the fields of cytology and immunology, and is mainly used in animal experiments to study the effect of NK cells on preventing the occurrence of melanoma. In particular, an experimental method for NK cells to prevent the occurrence of melanoma. Background technique [0002] Natural killer cells (Nature killer cells), also known as NK cells, are a type of CD56 + , CD3 - Lymphocytes are not only important outward natural defense cells, but also regulatory cells of various immune cells in the body. They have the ability to kill tumor cells and virus-infected cells, and play an important role in the body's innate immunity and adoptive immunotherapy. Most clinical studies use CD56 isolated from the peripheral blood of cancer patients + , CD3 - The NK cells are then expanded in vitro and then reinfused into the patient. However, since most patients have defective NK cells, the clinical effect of immunotherapy is not ideal. The pa...

Claims

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Application Information

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IPC IPC(8): A61K35/17A61P35/00A61P37/04
CPCA61K35/17A61P35/00A61P37/04
Inventor 卢君君马洁赵刚许蕊詹举明
Owner TIANJIN KANGTING BIOLOGICAL ENG GRP CO LTD
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