Cell preservation solution and method of cell preservation by using cell preservation solution

A preservation solution and cell technology, which is applied in the preservation, application, and animal husbandry of human or animal bodies, can solve the problems of complex components of cell preservation solution, irregular operation methods, and reduced survival rate, and achieve low configuration costs, Easy-to-use and easy-to-operate effects

Inactive Publication Date: 2019-02-19
合肥诺森医学检验有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the existing defects, provide a cell preservation solution and a method for preserving cells using the cell preservation solution, which can effectively solve the problem that the cell preservation solution in the background technology not only has complex components, but also has a single function. In addition, When using the cell preservation solution to preserve the cells, due to the non-standard operation method, the survival rate of the cells is greatly reduced during the preservation process.

Method used

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Examples

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Effect test

Embodiment 1

[0018] The present invention provides a technical solution: a cell preservation solution, including methanol, proclin300, EDTA, NaCl, acetic acid, albumin, glucose, normal saline and chromium chloride, characterized in that it is prepared from the following components by weight: Obtained: methanol: 15.00%-35.00%, proclin300: 0.20%-0.35%, EDTA: 0.10%-0.52%, NaCl: 0.10%-0.23%, acetic acid: 53.20%-68.70%, albumin: 2.30%-25.0% , Glucose: 0.10%-1.00%, normal saline 0.5-0.9% and chromium chloride 0.1%-1%.

[0019] Preferably, the preservation method of the cell preservation solution is as follows: as a preferred technical solution of the present invention, quality control cells are added to the above cell preservation solution, the quality control cells are Hela cells, and the concentration of the quality control cells added is 2 ×10 6 Cells / ml, 100ul, the temperature is controlled within the range of 2-8°C, after seven days of storage, the relative deviation of the number of cells...

Embodiment 2

[0028] The invention provides a technical solution: a cell preservation solution prepared from the following components in parts by weight: methanol: 18.00%-33.00%, proclin300: 0.28%-0.30%, EDTA: 0.22%-0.42%, NaCl : 0.15%-0.20%, acetic acid: 55.38%-60.21%, albumin: 10.30%-18.91%, glucose: 0.34%-0.85%, normal saline 0.7-0.8%, and chromium chloride 0.1%-0.5%.

[0029] Preferably, the preservation method of the cell preservation solution is as follows: as a preferred technical solution of the present invention, quality control cells are added to the above cell preservation solution, the quality control cells are Hela cells, and the concentration of the quality control cells added is 2 ×10 6 Cells / ml, 100ul, the temperature is controlled within the range of 2-8°C, after seven days of storage, the relative deviation of the number of cells from the number of cells just added is not more than 40%, and the pH of the above-mentioned cell preservation solution should be controlled at 4....

Embodiment 3

[0038] The invention provides a technical solution: a cell preservation solution prepared from the following components in parts by weight: methanol: 25%, proclin300: 0.29%, EDTA: 0.39%, NaCl: 0.18%, acetic acid: 58.45%, Albumin: 15.35%, glucose: 0.56%, physiological saline 0.61%, and chromium chloride 0.3%.

[0039]Preferably, the preservation method of the cell preservation solution is as follows: as a preferred technical solution of the present invention, quality control cells are added to the above cell preservation solution, the quality control cells are Hela cells, and the concentration of the quality control cells added is 2 ×10 6 Cells / ml, 100ul, the temperature is controlled within the range of 2-8°C, after seven days of storage, the relative deviation of the number of cells from the number of cells just added is not more than 40%, and the pH of the above-mentioned cell preservation solution should be controlled at 4.0-7.0 within the range

[0040] Preferably, 24 ho...

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PUM

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Abstract

The invention discloses a cell preservation solution, which is characterized by comprising, by weight, the following materials: 15.00-35.00% of methanol, 0.20-0.35% of proclin300, 0.10-0.52% of EDTA,0.10-0.23% of NaCl, 53.20-68.70% of acetic acid, 2.30-25.0% of albumin, 0.10-1.00% of glucose, 0.5-0.9% of normal saline and 0.1-1% of chromium chloride. By using the method, the collected cells can be well preserved in practice, and the morphology, activity and structure features of the original cells can be kept completely without the phenomenon of autolysis or burst of the cells. In addition, the cell preservation solution is simple in composition, low in cost, simple in use and convenient to operate. The cell preservation solution is suitable for promotion and used.

Description

technical field [0001] The invention relates to the technical field of cell preservation, in particular to a cell preservation solution and a method for preserving cells using the cell preservation solution. Background technique [0002] At present, normal human living cells have physiological functions, biological activities and complete cell morphology before shedding, which is of great value in biological research and medical testing. If the cells leave the human body, the physiological function, biological activity and cell shape structure of the exfoliated cells will also change. Therefore, when the cells are separated from the human body, it is necessary to use the cell preservation solution to monitor the shape, activity and structure of the cells after separation from the human body. maintain. [0003] However, the commonly used cell preservation solution is not only complicated in composition, but also has a single function. In addition, when using the cell preserv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 杨玉坤
Owner 合肥诺森医学检验有限公司
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