Method for storing biological samples and kit
A technology of biological samples and complexing agents, applied in biochemical equipment and methods, microbiological determination/inspection, etc., can solve the problems of FTA card direct amplification technology limitations, unfavorable sample collection and transfer, pH value change of filter paper cards, etc. , to achieve fast, safe and effective storage, high sample transfer efficiency, and easy operation
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Embodiment 1
[0071] Embodiment 1: The method for preserving biological samples according to the present invention preserves nucleic acids at room temperature
[0072] Disposable sampling tool: Medical cotton swab (Robust Medical)
[0073] Sample source: 9947 standard DNA solution (30ng / μL)
[0074] Functional solution: Tris at a concentration of 480mM pH 8.0-9.5, 30mM EDTA, 90mg / mL SDS, 60mM Na 2 SO 3 and 0.02% thymol blue
[0075] Filter paper card: Wohua filter paper (34mm×35mm)
[0076] Sample collection: put the disposable sampling tool in the EP tube containing 100 μL 9947 standard DNA solution to make the solution completely adsorbed; then put the disposable sampling tool adsorbed with the DNA solution in the EP tube containing 100 μL functional solution The solution is completely absorbed; remove the disposable sampling kit and transfer to a filter paper card. The filter paper cards were placed at room temperature for 0 days, 4 days, 8 days, 12 days, 16 days, 20 days, 24 days, ...
Embodiment 2
[0095] Embodiment 2: The method for preserving biological samples according to the present invention preserves nucleic acids at room temperature
[0096] Disposable sampling tool: Medical cotton swab (Robust Medical)
[0097] Sample source: 9947 standard DNA solution (30ng / μL)
[0098] Functional solution: Tris at a concentration of 240mM pH 8.0-9.5, 15mM EDTA, 45mg / mL SDS, 30mM Na 2 SO 3 and 0.02% thymol blue
[0099] Filter paper card: Wohua filter paper (34mm×35mm)
[0100] Sample collection: put the disposable sampling tool in the EP tube containing 100 μL 9947 standard DNA solution to make the solution completely adsorbed; then put the disposable sampling tool adsorbed with the DNA solution in the EP tube containing 100 μL functional solution The solution is completely absorbed; remove the disposable sampling kit and transfer to a filter paper card. The filter paper cards were left at room temperature for 0 days, 4 days, 8 days, 12 days, 16 days, 20 days, 24 days, an...
Embodiment 3
[0103] Embodiment 3: Antibacterial detection of the method for preserving biological samples according to the present invention
[0104] Disposable sampling tool: Medical cotton swab (Robust Medical)
[0105] Source of bacteria: XL10-gold Escherichia coli (engineering bacteria),
[0106] Functional solution composition:
[0107] Solution 1: Tris at a concentration of 240mM pH 8.0-9.5, 15mM EDTA, 45mg / mL SDS, 30mM Na 2 SO 3 and 0.02% thymol blue.
[0108] Solution 2: Tris at a concentration of 480mM pH 8.0-9.5, 30mM EDTA, 90mg / mL SDS, 60mM Na 2 SO 3 and 0.02% thymol blue.
[0109] Filter paper card: Wohua filter paper (34mm×35mm)
[0110] Sample collection: Put the disposable sampling tool in the EP tube containing 100 μL of bacterial suspension to make the solution completely adsorbed; then place the disposable sampling tool with adsorbed bacterial suspension in the EP tube containing 100 μL of different functional solutions, Allow the solution to absorb completely; re...
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