Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Sperm washing liquid as well as preparation method and application thereof

A washing liquid and sperm technology, which is applied in the field of sperm treatment, can solve the problems of low vitality and insufficient washing of harmful substances, and achieve the effects of maintaining activity, easy industrial production, and preventing damage

Active Publication Date: 2019-02-15
太东(镇江)生物科技有限公司
View PDF1 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The main purpose of the present invention is to solve the problems in the prior art that the vitality of sperm is not high after washing, and the washing of harmful substances is not sufficient, and provide a sperm washing culture solution and its preparation method and application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Sperm washing liquid as well as preparation method and application thereof
  • Sperm washing liquid as well as preparation method and application thereof
  • Sperm washing liquid as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Preparation of sperm washing solution (take 10L as an example)

[0053] A. Prepare according to the components in the table below, prepare the solid first and then the solution, and filter it with 0.22μm after the volume is reached to 10L.

[0054] Table 1 Components

[0055]

[0056]

[0057] Concrete preparation steps are as follows:

[0058]a. Weigh various inorganic salt solids (sodium chloride, potassium chloride, magnesium sulfate, calcium chloride, sodium bicarbonate, potassium dihydrogen phosphate, sodium pyruvate, sodium lactate, glucose and HEPES) accurately, and use ultrapure Dissolve in water for injection.

[0059] b. Filter the above liquid through a 0.22 μm filter membrane.

[0060] c. Amino acids (glycine, glutamine, L-alanine, L-aspartic acid, L-asparagine, L-glutamic acid, L-proline, L-serine, taurine), vitamins (vitamin E, vitamin C, vitamin B12) and gentamicin. Measure the osmotic pressure and pH value of the solution. At this time, the os...

experiment example 1

[0078] The sperm washing liquid that embodiment 1 makes also carries out following detection:

[0079] 1. Cytotoxicity test of sperm washing solution

[0080] The cytotoxicity test is carried out according to GB / T 16886.5. The toxicity test score of the finished sperm washing liquid is 0.2, less than 1 point, and the toxicity test of the washing liquid is qualified.

[0081] 2. Allergy detection

[0082] According to GB / T 16886.10, the sensitization test was carried out, and 20 adult mice were injected with the washing solution, and there was no abnormality or allergic reaction after 20 minutes.

[0083] 3. Bacterial endotoxin detection

[0084] The limulus reagent gel method is used for detection, and the detection value is 0.5EU / ml, which is less than the specified 1EU / ml, and the product is qualified.

experiment example 2

[0086] Carry out sperm motility experiment to the sperm washing liquid that embodiment 1 and comparative example 1-3 make

[0087] Preheat the sperm washing solution to about 37°C in advance;

[0088] Take 1ml of fresh semen, divide it into four parts, add 0.5ml of different sperm washing solution to each part, mix gently, centrifuge at 1000 rpm for 5 minutes, and remove the supernatant.

[0089] Add 1ml of washing solution again, mix gently, place in 37°C water bath for 30 minutes, then draw 0.4ml of liquid from the upper layer (sperm motility in this liquid is 100%) and put it into a new centrifuge tube, add 2ml of preheated washing solution, 37°C without CO 2 Cultivate for 24h.

[0090] Sperm motility was detected under a 200-fold microscope, three fields of view were randomly counted, and the total number of motile and immotile sperm was recorded.

[0091] After testing, in the sperm washing solution provided in Example 1, the total number of sperm in each visual field ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of sperm treatment, and specifically relates to sperm washing liquid as well as a preparation method and application thereof. The sperm washing liquid comprises thefollowing components: sodium chloride, potassium chloride, magnesium sulfate, calcium chloride, sodium bicarbonate, monopotassium phosphate, sodium pyruvate, sodium lactate, glucose, HEPES, glycine, glutamine, L-alanine, L-asparaginic acid, L-asparagine, L-glutamic acid, L-proline, L-serine, vitamin E, vitamin C, vitamin B12, taurine, gentamicin and human serum albumin. The sperm washing liquid iscomplete in components; all components are coordinated and matched with each other; nutrition energy can be effectively supplied; the oxidization reaction is inhibited; the sperm activity is maintained; the sperm survive rate is increased.

Description

technical field [0001] The invention relates to the field of sperm treatment, in particular to a sperm washing liquid and its preparation method and application. Background technique [0002] With the social environment and the development of late marriage and late childbearing, the infertility rate is increasing year by year, prompting the development of assisted reproductive technologies such as test-tube baby or in vitro fertilization. These techniques need to collect high-quality sperm, and the quality of sperm determines the success or failure of assisted reproduction. At present, there are two main sources of sperm collection, one is to directly collect human sperm, and the other is to extract from sperm banks. Both sources require the sperm to be washed before manipulation to remove poor or dead sperm. At the same time, it is necessary to obtain sufficient energy for high-quality sperm and remove harmful components in semen or cryopreserved fluid. [0003] At prese...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/076A01N1/02
CPCA01N1/0221C12N5/061C12N2500/12C12N2500/14C12N2500/16C12N2500/30C12N2500/32C12N2500/34C12N2500/38C12N2501/998
Inventor 李兵
Owner 太东(镇江)生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com