Vaccine strain rSN-R92G-E93K, construction method and applications thereof

Abstract

The invention provides a vaccine strain rSN-R92G-E93K, and belongs to the technical field of influenza vaccine preparation. The construction method comprises: preparing an expression vector pHW-SN-HAfrom a HA gene fragment; carrying out point mutation on the expression vector; co-transforming cells by using the obtained expression plasmid SNHA-R92G-E93K and plasmids containing other genes; and carrying out recombination. According to the present invention, the vaccine strain rSN-R92G-E93K has the HI titer of 11.6+ / -0.5 log2 and the serum neutralizing titer of 296.50+ / -103.95 log2, such that the vaccine strain rSN-R92G-E93K is an ideal vaccine strain.

Description

technical field [0001] The invention belongs to the technical field of influenza vaccine preparation, and in particular relates to a vaccine strain rSN-R92G-E93K and its construction method and application. Background technique [0002] H9 subtype low pathogenic avian influenza virus (Low Pathogenic Avian influenza virus, LPAIV), although the lethality to poultry is low, but it can lead to a sharp drop in egg production, while causing immunosuppression, such as mixed infection can cause higher Mortality caused huge economic losses to my country's poultry industry. The H9 subtype AIV will also serve as the donor of the internal genes of other subtype influenza viruses, providing conditions for the generation of new influenza virus strains, triggering the outbreak of new influenza epidemics, and posing a potential threat to public health security, such as the H9N2 subtype AIV in 2013 The new H7N9 virus that has been prevalent in my country since 2010 provides 6 internal gene se...

AI Technical Summary

Problems solved by technology

At present, multiple amino acid positions related to antigenicity of H9 subtype HA gene have been identified, but the impact of these antigenic epitopes on H9 antigenicity and how to design vaccines have not yet been studied.

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