Method for extracting ceramide from pineapples and application

Abstract

The invention discloses a method for extracting ceramide from pineapples and application, and relates to the technical field of biological active substance preparation and application. The method comprises the steps that pineapple leaves and pineapple peel of immature pineapples are selected, the process routes for pineapple picking and preprocessing, freezing, drying, smashing, extracting, concentrating, extracting, separating, column chromatography isolation, gradient elution and re-crystallization are designed, the process design is reasonable, the toxicity of adopted solvents is small, noharsh condition is adopted, a high-purity N-fatty acyl-sphingol substance can be obtained, on the basis of NMR, MS and HPLC detection, the detection result displays that the N-fatty acyl-sphingol substance is 1-O-beta-D-glucose-N-docosanedioic acyl-hexadecanoic-4,10(E,E)-diene psychosine, the purity reaches 98.7%, the yield is increased to 0.13% from 0.0004%, and the method is suitable for large-scale technological production.

Description

technical field [0001] The invention relates to the technical field of preparation and application of bioactive substances, in particular to a method and application of extracting ceramide from pineapple. Background technique [0002] Ceramide (Ceramide), that is, N-ester acyl (nerve) sphingosine, is an important component of the stratum corneum of the skin. It has the functions of barrier, adhesion, moisturizing, anti-aging, anti-allergy, etc. Daily chemical, medical and cosmetic industries. The latest research has found that ceramide can participate in intracellular signal transduction by activating various protein kinases and transcription factors, thereby affecting various physiological and pathological processes such as cell growth, proliferation, differentiation, apoptosis and injury. As a new type of biologically active substance, it has broad application prospects in the medicine, health care and cosmetic industries. [0003] At present, due to the diversity of cer...

AI Technical Summary

Problems solved by technology

[0006] It is found through experiments that the above-mentioned method has the following problems: 1. After the pineapple leaves are washed with water and dried at high temperature, they are extracted with high-concentration 80% ethanol under reflux, without steps such as cutting and crushing, the active substances in the pineapple leaves cannot be fully released by breaking the wall , leading to a great reduction in the extracted active substances, and high temperature drying is also easy to destroy the high temperature sensitization active substances, resulting in the denaturation of the active substances and the increase of compounds, which increases the difficulty of separation and purification; - Methanol gradient elution, in the process of silica gel sample mixing and elution, not only greatly dilute the concentration of N-ester ceramides, but also chloroform and methanol are highly toxic and use a large amount, which increases the extraction cost and cannot be realized Industrialized production; 3, did not utilize the fresh pineapple peel part to extract, caused the waste of resources, reduced the yield of N-ester acyl ceramides