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Construction method of a model for cynomolgus monkey osteoporosis caused by oophorectomy

A technology for ovariectomy and osteoporosis, applied in the field of biomedical research, can solve the problems of inconvenience, life-threatening, slow treatment effect, etc.

Inactive Publication Date: 2019-01-22
澎立生物医药技术(上海)股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Osteoporosis brings great inconvenience and pain to the life of patients, the treatment effect is very slow, and once a fracture occurs, it can be life-threatening

Method used

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  • Construction method of a model for cynomolgus monkey osteoporosis caused by oophorectomy
  • Construction method of a model for cynomolgus monkey osteoporosis caused by oophorectomy
  • Construction method of a model for cynomolgus monkey osteoporosis caused by oophorectomy

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1. Preoperative administration and anesthesia

[0029] Adult female cynomolgus monkeys, over 9 years old, the basic data will be tested after the animals are observed for 14 days during the adaptation period after receiving them. The selected animals are healthy, have normal menstrual cycles, no abnormal bones, closed epiphyseal plates, and serum and urine test indicators are in the normal range. Animals in the experiment.

[0030] The 12 cynomolgus monkeys were randomly divided into 2 groups, as shown in Table 1 below. The number of animals in the group was calculated according to statistics and with reference to the results of other long-term ovariectomized (OVX) cynomolgus monkey bone quality studies. The age and weight of the groups should be guaranteed , the balance of lumbar bone mineral content (BMC) and bone mineral density (BMD).

[0031] The first group was the bilateral ovariectomy group, and the second group was the sham group (sham group). Befo...

Embodiment 2

[0034] Example 2. Bilateral Ovariectomy

[0035] After the animals were anesthetized, the skin was disinfected with povidone iodine, up to the ribs, down to the pubic symphysis, and on both sides to the lateral abdominal wall. A medical surgical drape is spread on the abdomen and fixed by cloth forceps; a longitudinal incision of about 4 cm is made on the midline of the lower abdomen, and the subcutaneous tissue is bluntly separated to expose the midline of the abdomen, the peritoneum is incised at the linea alba, and one ovary is bluntly freed , lift the ovary, reveal the ovarian mesosalpinx, ovarian ligament proper, and keep the pelvic infundibulum ligament stretched. The pelvic infundibulum ligament blood vessels, mesentery and ovarian proper ligament were clamped along the base of the ovary, the ovary was completely removed, and the stump was sutured across. The other ovary was removed in the same way; the presence of active bleeding was checked, and if there was no bleed...

Embodiment 3

[0037] Example 3. Postoperative care

[0038] All animals were given 0.02 mg / kg of analgesic buprenorphine for 3 consecutive days after operation, three times a day; if necessary, an additional time could be given. After the operation, all animals were given 50 mg / kg of antibiotic ceftriaxone sodium for 3 consecutive days.

[0039] During the recovery period from anesthesia, animals were allowed to move freely and bear full weight. Animal experimenters must closely monitor all animals during the recovery period from anesthesia. Food and water were placed on the bottom of the cage as much as possible to ensure that the animals had access to food and water after surgery; a certain amount of fruits and vegetables were given after surgery to stimulate the animal's appetite. Animals were monitored for wound healing and overall mental status twice a day for 3 consecutive days, and any discomfort, pain, limitation of limb movement and other surgical-related vital signs were recorded...

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Abstract

The invention relates to the technical field of biomedical research, and in particular relates to a construction method of a model for cynomolgus monkey osteoporosis caused by oophorectomy. The technical scheme is as follows: the cynomolgus monkey osteoporosis model is established through bilateral oophorectomy, then, through detection on serum bone conversion related indicators, lumbar vertebra bone densitometry, cancellous bone tissue morphologic and metrological testing and lumbar vertebra compression testing, the bone quality in each group is evaluated, the parameters show that due to theoophorectomy surgery, the bone absorption and bone formation parameters are increased, the bone mass and bone density are obviously reduced, and the mechanical strength is reduced. With the method, the model for cynomolgus monkey osteoporosis caused by oophorectomy can be effectively established, and reliable data for the treatment process is provided for further studying the osteoporosis of postmenopausal women medically.

Description

technical field [0001] The invention relates to the technical field of biomedical research, in particular to a method for constructing a cynomolgus monkey osteoporosis model caused by ovariectomy. Background technique [0002] Osteoporosis is a disease of low bone mass, prone to fractures, and is more common in the elderly and menopausal women. The reason is that the bone microstructure becomes thinner and thinner, bone salt and organic matter decrease at the same time, which may affect the occurrence of multiple fractures in the systemic bones. The underlying mechanism is primarily caused by an imbalance in bone formation, ie, osteoblast activity, and bone resorption, ie, osteoclast activity. Osteoporosis brings great inconvenience and pain to the life of patients, the treatment effect is very slow, and once a fracture occurs, it can be life-threatening. In senile osteoporosis, the vertebral body is compressed and deformed, the spine bends forward, muscle fatigue and even...

Claims

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Application Information

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IPC IPC(8): A61D1/00A61D7/00
CPCA61D1/00A61D7/00
Inventor 袁红江刘萧陈刚杨楠曾佳李娇娇
Owner 澎立生物医药技术(上海)股份有限公司
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