Preparation method of soft-shelled turtle peptide

Abstract

The invention discloses a preparation method of soft-shelled turtle peptide. The method comprises the steps of: pulverizing the raw material of the turtle, adding an aqueous solution containing 3,4,5-trihydroxy-1-cyclohexene-1- formic acid and puerarin and putting them into the twin-screw cooking heat together; In the feed inlet of the extruder, the thick slurry is hot extruded, and the papain restriction enzyme is added, the enzymatic hydrolysate is centrifuged, the supernatant is collected, and the dried turtle collagen is freeze-dried; the turtle collagen and Bacillus subtilis are collected. The protease is dissolved in distilled water, and then t-butanol and stevioside are added in sequence to hydrolyze the enzymatic hydrolysate; the enzymatic hydrolysate is decolorized, ultrafiltration membrane is removed, nanofiltration membrane is concentrated, and freeze-dried to obtain turtle collagen peptide. The beneficial effects are as follows: the preparation method of the turtle peptideof the invention does not require acid-base pretreatment, the purity of the turtle protein is high, the extraction rate of the turtle peptide is high, and the extraction time is short.

Description

technical field [0001] The invention relates to the technical field of soft-shelled turtle deep processing, in particular to a preparation method of soft-shelled turtle peptide. Background technique [0002] Soft-shelled turtle, also known as soft-shelled turtle and soft-shelled turtle, has the effects of nourishing blood, strengthening bones, anti-fatigue, and prolonging life. It is a traditional food in my country and a good raw material for developing high-level health food; Glue, cutin white and multivitamins, etc. Turtle contains high protein, about 165 grams of protein per kilogram, and the protein in food or medicine is generally absorbed and utilized by the body by enzymatic hydrolysis into oligopeptides or amino acids by the digestive juice, so the absorption is slow and the utilization rate is low. Therefore, the desired medicinal effect cannot be achieved. [0003] Collagen peptide is a hydrolyzed product of collagen, generally with a molecular weight below 3000D...

AI Technical Summary

Problems solved by technology

[0005] The prior art, such as the Chinese invention patent with the authorized announcement number CN 102286589B, discloses a method for extracting soft-shelled turtle oligopeptides. After the soft-shelled turtle is used as raw material to enzymolyze with alkaline protease, flavor protease is used to exocut and adjust the flavor. The liquid solution is subjected to ultrafiltration, the amount of alkaline protease added is 0.2-5% of the weight of the raw soft-shelled turtle, the amount of flavored protease added is 0.1-2% of the weight of the raw soft-shelled turtle, and the ultrafiltration membrane with a membrane molecular weight cut-off of 0.5-300,000 Daltons Ultrafiltration, film concentration and spray drying are used to obtain soft-shelled turtle oligopeptides; the invention is easy to operate, has a good taste, is low in cost, is environmentally friendly and efficient, and is very suitable for large-scale industrial production. Incomplete hydrolysis reduces the yield of soft-shelled turtle protein peptides, resulting in waste of nutrients