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Rapid preparation method of paraffin sections of pear tissue

A technology of paraffin sectioning and production method, which is applied in the field of histology, and can solve problems such as difficulty in achieving expected results, long time-consuming section production, and long time-consuming time, so as to ensure the effect of wax dipping, improve the quality of section production, and overcome adverse effects Effect

Inactive Publication Date: 2019-01-15
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional paraffin sections take a long time to make, and it is difficult to achieve the desired effect on dense tissue wax immersion. At present, microwave radiation fast paraffin sections are widely used in medical tissue sections, and are less used in plant tissue sections. There are related reports in the leaves
[0003] Due to the dense texture and fluff in the flower buds of pear annual branches, it is difficult to achieve the expected results due to the poor wax dipping effect when making traditional paraffin slices.
At the same time, due to the long time-consuming, few people carry out histology and developmental biology research through the method of paraffin section
[0004] To sum up, the defects of the existing technology are: it takes a long time to make slices; the effect of wax dipping in dense tissues is not good, and the obtained slices are not clear

Method used

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  • Rapid preparation method of paraffin sections of pear tissue
  • Rapid preparation method of paraffin sections of pear tissue
  • Rapid preparation method of paraffin sections of pear tissue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A kind of rapid preparation method of pear tissue paraffin section, comprises the following steps:

[0039] S1, material collection and fixation: cut annual pear branches with a middle length of 0.5 cm, put them into FAA fixative solution with a volume fraction of 70%, and fix them to obtain fixed pear tissues;

[0040] S2, Dehydration: Take out the fixed pear tissues from the FAA fixative, and dehydrate them with ethanol solutions with volume fractions of 70%, 85%, 95%, 100%, and 100% in sequence. The dehydration process is carried out in a microwave oven at 65°C. The immersion time of integral ethanol solution is 10min;

[0041]S3, transparent: the pear tissue after dehydration, and then poured into the mixed solution with a volume ratio of ethanol:xylene=1:1, xylene, and xylene in sequence, placed in a microwave oven at 65°C, and soaked for 10 minutes each;

[0042] S4, soaking in wax: the transparent pear tissue is soaked in a mixed solution with a volume ratio of ...

Embodiment 2

[0048] A kind of rapid production method of paraffin section of pear tissue, described pear tissue section production method is the same as embodiment 1, difference is: (1) material in S1 is the middle part leaf of pear branch, and the area near the main vein of the leaf is cut with a puncher 0.5cm 2 (2) In S2, the dehydration process was carried out in a 65°C constant temperature water bath; the immersion time of each volume fraction of ethanol solution was 5min; (3) in S3, the transparent process was carried out in a 65°C constant temperature water bath; Soak for 5 minutes each time; (4) In S4, the wax immersion process is carried out in a constant temperature water bath at 65°C; soak for 20 minutes each time.

Embodiment 3

[0050] A quick preparation method for paraffin slices of pear tissue, the preparation method of pear tissue slices is the same as in Example 1, the difference is that: (1) the material in S1 is pear flower buds stripped of external scales; (2) each volume fraction of ethanol in S2 The soaking time of the solution is 7 minutes; (3) each soaking time in S3 is 8 minutes; (4) each soaking time is 25 minutes in S4.

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Abstract

The invention discloses a rapid preparation method of paraffin sections of pear tissue, comprising the following steps: S1, taking and fixing materials; S2, dewatering; S3, carrying out transparence treatment; S4, wax-dipping; S5, embedding; S6, slicing, pasting and drying; S7, dewaxing, rehydrating and dyeing; S8, dewatering, carrying out transparence treatment and sealing, wherein the dewatering, transparence treatment and wax-dipping processes are carried out in a constant temperature environment of 65 DEG C through microwave radiation heating or water bath heating. According to the rapid preparation method of paraffin sections of pear tissue, the manufacturing time of the paraffin sections of the pear tissue can be greatly shortened, the obtained sliced tissue is clear, and the qualityof the sections can reach the expected effect.

Description

technical field [0001] The invention relates to the field of histology, in particular to a rapid preparation method for paraffin sections of pear tissue. Background technique [0002] Paraffin sections are used in many fields such as botany, zoology, and medicine, and are the main experimental methods for histology and developmental biology research. Traditional paraffin sections take a long time to make, and it is difficult to achieve the desired effect on dense tissue wax immersion. At present, microwave radiation fast paraffin sections are widely used in medical tissue sections, and are less used in plant tissue sections. They are only used in corn and rice. There are related reports in the leaves. [0003] Due to the dense texture and fluff in the flower buds of pear annual branches, it is difficult to achieve the expected results due to the poor wax dipping effect when traditional paraffin slices are made. At the same time, due to the long time consumption, few people...

Claims

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Application Information

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IPC IPC(8): G01N1/06G01N1/28
CPCG01N1/06G01N1/28G01N1/30
Inventor 王强张茂君武春昊卢明艳闫兴凯刘明鹤
Owner JILIN ACAD OF AGRI SCI
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