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A bovine bone collagen peptide and a preparation method thereof and use

A technology of bone collagen peptides and collagen peptides, applied in the fields of food engineering and biology, can solve the problems of insufficient extraction rate of bovine collagen peptides, insufficient small molecule active peptides, etc., and achieve the effect of small molecular weight

Inactive Publication Date: 2019-01-15
内蒙古蒙肽生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The bovine bone collagen peptide extraction rate obtained by the above method is not enough, and the small molecule active peptide is insufficient

Method used

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  • A bovine bone collagen peptide and a preparation method thereof and use
  • A bovine bone collagen peptide and a preparation method thereof and use
  • A bovine bone collagen peptide and a preparation method thereof and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Preparation:

[0040] (1) Take a certain amount of fresh beef bones that have passed inspection and quarantine, crush them, wash them, add an equal volume of water, and cook them for 0.5 hours.

[0041] (2) Discard the beef bone cooking liquid obtained in step (1), take out the beef bone, remove the meat, fascia, and fat in the bone cavity attached to the bone, and then wash it with hot water twice until the bone turns white Or milky white, no oil visible to the naked eye, to get clean beef bones.

[0042] (3), pulverize the bovine bone obtained in the step (2), and cross a 100-mesh standard sieve to obtain bovine bone fine powder.

[0043] (4), adding 5 times the quality of water to the bovine bone fine powder obtained in step (3), high temperature and high pressure for 40 minutes, filtering to get the supernatant to obtain bovine bone collagen liquid.

[0044] (5) Add 2% trypsin to the bovine bone collagen solution obtained in step (4), adjust the pH value to 8.0, a...

Embodiment 2

[0050] Preparation:

[0051] (1) Take a certain amount of fresh beef bones that have passed inspection and quarantine, crush them, wash them, add an equal volume of water, and cook for 1 hour.

[0052] (2) Discard the beef bone cooking solution obtained in step (1), take out the beef bone, remove the meat, fascia, and grease in the bone cavity attached to the bone, and then wash with hot water for 3 times until the bone turns white Or milky white, no oil visible to the naked eye, to get clean beef bones.

[0053] (3), pulverize the bovine bone obtained in the step (2), and cross a 100-mesh standard sieve to obtain bovine bone fine powder.

[0054] (4), add the bovine bone fine powder that obtains in step (3), add the water of 6 times of quality, high temperature and high pressure 40min, filter and take supernatant, obtain bovine bone collagen protein liquid.

[0055] (5) Add 0.5% trypsin to the bovine bone collagen solution obtained in step (4), adjust the pH value to 7.0, a...

Embodiment 3

[0059] Preparation:

[0060] (1) Take a certain amount of fresh beef bones that have passed inspection and quarantine, crush them, wash them, add an equal volume of water, and cook for 1.5 hours.

[0061] (2) Discard the beef bone cooking solution obtained in step (1), take out the beef bone, remove the meat, fascia, and grease in the bone cavity attached to the bone, and then wash with hot water for 3 times until the bone turns white Or milky white, no oil visible to the naked eye, to get clean beef bones.

[0062] (3), pulverize the bovine bone obtained in the step (2), and cross a 100-mesh standard sieve to obtain bovine bone fine powder.

[0063] (4) Add 10 times the mass of water to the fine bovine bone powder obtained in step (3), subject it to high temperature and high pressure for 2 hours, filter to get the supernatant, and obtain a bovine bone collagen liquid.

[0064] (5) Add 1.5% trypsin to the bovine bone collagen solution obtained in step (4), adjust the pH valu...

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Abstract

The invention relates to the field of food engineering and biotechnology, in particular to a bovine bone collagen peptide and a preparation method and use thereof. The method includes crushing bovinefresh bone, removing fascia and bony flesh by complete physical method, skimming to obtain white or milky white clean bovine bones, pulverizing the bovine bones, sieving with 100 mesh sieve to obtainbovine bone powder, steaming the bovine bone powder under high pressure, extracting collagen, and hydrolyzing the mixture of trypsin and compound protease-an alkaline protease, neutral protease and maltodextrin to obtain bovine bone peptide stock solution step by step. For the first time, trypsin and compound protease are used to hydrolyze bone powder twice, so that the molecular weight of more than 85% collagen peptide could be less than 1000 Da without separation of the peptide.

Description

technical field [0001] The invention relates to the fields of food engineering and biotechnology, in particular to a bovine bone collagen peptide, its preparation method and application. Background technique [0002] At present, the aging of the population has become a global phenomenon. Along with the aging of the population, the number of patients with osteoporosis has also increased sharply. Osteoporosis has become a serious problem worldwide. [0003] Osteoporosis is a disease in which bone mass decreases throughout the body, and bone microstructure is destroyed, resulting in increased bone fragility, resulting in a susceptibility to fracture during minor trauma. The term osteoporosis was first proposed by Pommer in 1885, which means a disease of bone loss. Osteopenia is a feature of physiological aging that occurs with increasing age. Bone is formed by the deposition of crystals composed of calcium ions and phosphorus on a matrix composed of collagen. The ratio of bone...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78A23L33/18A61K38/39A61P19/10
CPCA23L33/18A23V2002/00A61K38/00A61K38/39A61P19/10C07K14/78C12P21/06A23V2200/306A23V2250/55
Inventor 逯振宇温宇旗陈丽娜韩国庆王继明王耀新杜芳魏鹏飞
Owner 内蒙古蒙肽生物工程有限公司
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