Type A foot-and-mouth disease virus sIgA antibody ELISA kit and application thereof

Abstract

The invention discloses a type A foot-and-mouth disease virus sIgA antibody ELISA kit and application thereof. The kit comprises an enzyme-labeled reaction plate coated with a type A foot-and-mouth disease virus broad-spectrum multi-epitope recombinant antigen, a 100x concentrated enzyme-labeled antibody, an enzyme-labeled antibody diluent, a sample diluent, a washing concentrate, a developing solution, a stop solution, a positive control sample and a negative control sample. The type A foot-and-mouth disease virus broad-spectrum multi-epitope recombinant antigen consists of dominant antigen epitopes (TB / A) of three separated representative type A foot-and-mouth disease strains of China, so that the detection sensitivity and specificity of the kit are improved, and the kit is applied to detection of infection by different type A foot-and-mouth disease viruses. The kit is suitable for detecting sIgA antibodies in mucosal secretions of three susceptible animals, i.e., pigs, cattle and sheep, and is significant for prevention and control of propagation and infection of type A foot-and-mouth disease viruses.

Description

technical field [0001] The invention relates to an indirect ELISA detection kit for foot-and-mouth disease type A virus sIgA antibody and a detection method thereof. The invention belongs to the technical field of biological detection. Background technique [0002] Foot-and-Mouth Disease (FMD) is an infectious disease caused by the Foot-and-Mouth Disease virus, characterized by vesicular disease on the mouth and hooves of sick animals. Foot-and-Mouth Disease Virus (FMDV) belongs to the Picornaviridae family, and the center of the virus is a single-stranded RNA. According to the serotype characteristics, it can be divided into 7 serotypes: A, O, C, SAT1, SAT2, SAT3 (ie South African 1, 2, 3) and Asian (Asian 1). There is almost no immune protection between them, and animals infected with one serotype of FMDV can still be infected with another serotype of FMDV to develop disease. There are more than 70 kinds of FMDV infection objects, the incubation period is 1-7 days, the ...

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Problems solved by technology

[0005] At present, there is no kit that can effectively detect sIgA antibody to foot-and-mouth disease virus in the market. In view of the in-depth research on mucosal immune mechanism and mucosal immune vaccine in recent years, an ELISA kit for detecting the level of sIgA antibody to foot-and-mouth disease virus in mucosal secretions can be developed. More accurately reflect the FMD virus infection and mucosal immune status, and use this method to monitor the dynamic changes of sIgA antibody to FMD virus, which provides theoretical and experimental basis for the FMD mucosal immunity vaccine and the establishment of supporting detection methods

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