High-throughout heatproof rice screening method

Abstract

The invention discloses a high-throughput heatproof rice screening method. The method comprises the following steps: (1) preparing F2 generation, DH line, BC1F2 or RIL seeds by using heat-sensitive rice in the flowering stage as a receptor and heatproof rice as a donor; (2) processing the seeds, carrying out tray close planting, cultivating seedlings to obtain a genetic segregation population, andplanting parents and F1 generation by using same processes; (3) reserving one effective tiller on every plant in the jointing and booting stage, carrying out heatproof screening treatment in an artificial climate room if the heading of the population is similar, and performing filing and fruit-setting in a normal environment when the heading and flowering of all the tillers of the populations arefinished; (4) performing pocket cutting and pile reserving after all plants are headed if the heading period is different significantly, and carrying out heading and heatproof screening; and (5) counting the heatproof fruit-setting rate after the dough stage of single spikes in order to complete the heatproof phenotype screening of the individual plants of the population. The method allows high-throughput heatproof screening to be quickly and reliably carried out by using the close planting control technology and pocket cutting.

Description

technical field [0001] The invention relates to a heat-resistant screening method, in particular to a high-throughput heat-resistant rice screening method. Background technique [0002] As the trend of global warming intensifies, summer heat damage occurs frequently in China, causing great harm to my country's summer crop production, especially the rice production in the Yangtze River Basin frequently encounters the threat of high temperature heat damage. In 2003, in Zhejiang, Anhui, Jiangsu, Hubei and other provinces along the Yangtze River Basin, the extreme high temperature at the heading stage of rice exceeded 40°C, and the continuous high temperature weather lasted for 14-16 days. Rice production generally decreased by 20%-30%, and economic losses were nearly 10 billion yuan. The high temperature and heat damage affected the national rice output that year and dropped to the lowest point in nearly 20 years. In 2013 and 2016, the rice fields in the Yangtze River Basin su...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a high-throughput heat-resistant rice screening method, which solves the problems that the existing method is difficult to screen the isolated generations of hybrid breeding in an artificial climate chamber, and the inconsistency of the heading date of the population, Using dense planting and tiller control technology, the planting area of ​​the group is significantly reduced, and the cutting pocket method is used to unify the heading time, which can quickly and reliably conduct heat-resistant screening and meet the high-throughput screening requirements