A kind of pear transcription factor pbbp and its application

A transcription factor and plant technology, applied in the fields of plant molecular biology and genetic engineering, to reduce the degree of lignification, improve the quality of pear pulp, and slow down the development of cell walls

Active Publication Date: 2022-02-18
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The structure of this gene family is conserved and widely distributed, and it exists in almost all plants, but so far there have been no related reports in pears, and there are few reports on the transcription factors that regulate lignin synthesis in pears

Method used

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  • A kind of pear transcription factor pbbp and its application
  • A kind of pear transcription factor pbbp and its application
  • A kind of pear transcription factor pbbp and its application

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Experimental program
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Embodiment Construction

[0027] Below in conjunction with accompanying drawing, the present invention is described in further detail:

[0028] A pear transcription factor PbBP of the present invention and its application, the methods used in the following examples are conventional methods unless otherwise specified, the primers used are all synthesized by Shanghai Sangon Bioengineering Technology Service Co., Ltd., and the sequencing is performed by Shanghai Sangon Biotechnology Conducted by Engineering Technology Services Ltd., pMD-18T, restriction enzymes Spe I and Bgl II, T 4 All ligases were purchased from Biotech (Beijing) Co., Ltd. (takara China). Both RNAprep Pure Plant Kit and FastQuant RTKit were purchased from TIANGEN Company. DNA gel recovery kit, plasmid extraction kit, and X-Gluc were purchased from Shanghai Sangon Bioengineering Technology Service Co., Ltd., and the methods were performed according to the instructions. The pear is Dangshan pear.

[0029] 1. PbBP gene cloning

[0030]...

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PUM

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Abstract

The invention discloses a pear lignin synthesis regulating PbBP gene, the pear lignin synthesis regulating PbBP gene has a nucleotide sequence as shown in SEQ ID NO.1, named as PbBP, and the amino acid sequence encoded by the PbBP gene As shown in SEQ ID NO.2, the nucleotide length is 1194bp, and the amino acid length is 397 amino acids. The regulatory gene PbBP and its coded protein are used in the expression of synthetic lignin genes in plants. The PbBP gene of the invention has a negative regulatory effect, and can directly inhibit the expression of plant lignin synthesis genes to hinder the biosynthesis of lignin, slow down the development of cell walls, reduce the degree of lignification, and improve the quality of pear pulp.

Description

technical field [0001] The invention belongs to the technical fields of plant molecular biology and genetic engineering, and specifically relates to a pear transcription factor PbBP and an application thereof. Background technique [0002] Pear belongs to Rosaceae (Rosaceae) pear subfamily (Pomaeeae) Pyrus L. plant, is one of important cultivated fruit trees in the world. Among them, Dangshansu pear, which is native to Dangshan County, Anhui Province, belongs to the white pear (Pyrus bretschneideri) system and is the pear variety with the largest cultivated area in my country. With the enrichment of fruit varieties and the improvement of consumers' living standards, the inherent defects of high content and large volume of Dangshan pear stone cell clusters have become increasingly prominent. The level of stone cell content, the size and density of stone cell clusters will not only affect the texture and taste of pear fruit, but also affect the sugar content of pear fruit. H...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/84C12N1/21A01H5/00A01H5/10A01H6/20
CPCC12N15/8255C07K14/415
Inventor 蔡永萍李曼丽程曦金青李大辉张金云林毅
Owner ANHUI AGRICULTURAL UNIVERSITY
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